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A kind of Lactobacillus acidophilus jyla-191 that reduces cholesterol content in blood and its application

A technology of JYLA-191 and Lactobacillus acidophilus, which is applied in the field of microorganisms, can solve problems such as adverse reactions and lower cholesterol levels, and achieve the effects of slowing down absorption, accelerating conversion into bile acid, and reducing content

Active Publication Date: 2021-05-28
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a Lactobacillus acidophilus JYLA-191 that lowers the cholesterol content in the blood and its application, mainly to solve the problem of adverse reactions caused by taking medicine to lower the cholesterol content in the blood

Method used

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  • A kind of Lactobacillus acidophilus jyla-191 that reduces cholesterol content in blood and its application
  • A kind of Lactobacillus acidophilus jyla-191 that reduces cholesterol content in blood and its application
  • A kind of Lactobacillus acidophilus jyla-191 that reduces cholesterol content in blood and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and screening of dominant lactic acid bacteria

[0030] ① Source of bacteria: healthy adult male feces.

[0031] ② Plate medium: peptone 10g, beef extract powder 8.6g, yeast extract powder 5g, K 2 HPO 4 2g, triammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.58g, MnSO 4 4H 2 O 0.25g, agar 15g, distilled water 1000mL.

[0032] ③Isolation of strains

[0033] In Bama, Guangxi, the collection personnel used a sterile stool collector to sample the stool of healthy adult men, wrapped it in a sterile ziplock bag, recorded the number, and transported it back to the company in a cold chain.

[0034] After the sample is taken out, wipe the surface of the sampler with 75% alcohol, inject 9mL of sterile water into the sampler in the ultra-clean workbench, mix well, and make a ten-fold gradient dilution of the sample liquid, and take 100μL for each gradient and evenly spread it on the MRS solid Plate culture medium, the...

Embodiment 2

[0040] Example 2 Control Experiment of JYLA-191 Cholesterol Clearance Rate

[0041] 1. Medium

[0042] (1) MRS liquid medium: peptone 10g, beef extract powder 8.6g, yeast extract powder 5g, K 2 HPO 4 2g, triammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2O 0.58g, MnSO 4 4H 2 O 0.25g, agar 15g, distilled water 1000mL.

[0043] (2) MRS-CHOL liquid medium: Weigh 54g of MRS medium, 1.0g of cholesterol, 20ml of Tween 80, and 3.0g of ox bile salt. Pour it into the culture medium, dilute to 1L, autoclave at 121°C for 20 minutes, cool and store for later use.

[0044] (3) Phthalaldehyde developer: 0.25mg / mL o-phthalaldehyde, solvent is glacial acetic acid.

[0045] 2. Strains

[0046] JYLA-191, ATCC13651, ATCC4357, ATCC4355, ATCC832, ATCC4356, ATCC4796.

[0047] 3. Operation

[0048] Cultured in MRS liquid medium at 37°C for 48h. The strain was activated twice, inoculated into MRS-CHOL medium at a volume percentage of 3%, and cultivated in a...

Embodiment 3

[0052] Example 3 JYLA-191 Bile Salt Hydrolase Activity Experiment

[0053] principle

[0054] Bile salt hydrolase (BSH) exists in intestinal microorganisms and can hydrolyze bound bile salts in the intestine to form amino acids and free bile acids. Bile acids cannot be reabsorbed in the large intestine and are excreted with feces. This leads to a reduction in the number of cycles of bile acids in the enterohepatic system, prompting more cholesterol to be metabolized to synthesize new bile salts, thereby reducing serum cholesterol levels.

[0055] strain

[0056] JYLA-191, ATCC13651, ATCC4357, ATCC4355, ATCC832, ATCC4356, ATCC4796.

[0057] operate

[0058] Add 0.3% (m / v) sodium deoxytaurocholate (TDCA), 0.2% (m / v) sodium thioglycolate, 0.37 g / L CaCl to MRS liquid medium 2 and 1.5% (m / v) agar, sterilized under high temperature and high pressure at 121°C for 15 minutes, poured into the sterilized plate, until the MRS solidified in the plate, and then put the sterile filter p...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a Lactobacillus acidophilus JYLA‑191 for lowering blood cholesterol levels and its application. Its classification is named Lactobacillus acidophilus (Lactobacillus acidophilus), and the preservation unit is: General Microbiology Center of China Microbiological Culture Collection Management Committee, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, zip code : 100101, its deposit number is: CGMCC No.21371, and its deposit date is December 14, 2020. The Lactobacillus acidophilus JYLA-191 bacterium of the present invention can combine with cholesterol in the intestinal tract, slow down the absorption of cholesterol, reduce the formation speed of lipoprotein in the blood, and reduce the content of cholesterol in the circulatory system.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus acidophilus JYLA-191 for reducing blood cholesterol levels and its application. Background technique [0002] Cholesterol is also known as cholesterol. A derivative of cyclopentane polyhydrophenanthrene. As early as the 18th century, people had discovered cholesterol from gallstones. In 1816, the chemist Bencher named this lipid-like substance cholesterol. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. Its solubility is similar to that of fat, insoluble in water, and easily soluble in solvents such as ether and chloroform. Cholesterol is an indispensable and important substance in animal tissue cells. It not only participates in the formation of cell membranes, but also is a raw material for the synthesis of bile acids, vitamin D and steroid hormones. Cholesterol ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06C12R1/23
CPCA61K35/747A61P3/06C12N1/20
Inventor 潘仕城司书锋周文浩李翠华潘玉林曹维超侯建亮孙川
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH