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A preparation method and product of a highly stable vegetable oil seed fermentation self-packaging carrier and its application

A high-stability, plant-based technology, applied in the field of skin care products, can solve problems such as incompatibility of vegetable oils, achieve the effect of improving economic application value, no adverse reactions, and no hemolysis

Active Publication Date: 2021-10-22
RAYTING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most vegetable oils are extracted through chemical and physical processes, and other rich proteins and water-soluble vitamins contained in vegetable oils are discarded; while traditional biological fermentation methods are mostly carried out in the water phase, the traditional fermentation process and Vegetable fats are not compatible

Method used

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  • A preparation method and product of a highly stable vegetable oil seed fermentation self-packaging carrier and its application
  • A preparation method and product of a highly stable vegetable oil seed fermentation self-packaging carrier and its application
  • A preparation method and product of a highly stable vegetable oil seed fermentation self-packaging carrier and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Camellia seed fermented from the package

[0041] 1. Preparation of seed solution

[0042] Under aseptic conditions, a single colony of Saccharomyces cerevisiae was picked and inoculated into YPD liquid medium. 30℃, 200r·min -1 Shake culture for 24 hours until OD600 is about 16 to obtain seed solution.

[0043] 2. Prepare materials

[0044] Wash the camellia seeds with deionized water, drain; add to the wall breaking machine, add 3 times the mass ratio of deionized water, and crush until no large solid particles are visible.

[0045] 3. Fermentation substrate preparation

[0046] Camellia seeds 40g·L -1 , glucose 20g·L -1 . Sterilize at 121°C for 20 minutes to obtain a fermentation substrate, which is then cooled for use.

[0047] 4. Camellia seed fermentation

[0048]Add Saccharomyces cerevisiae seed solution with a volume ratio of 5% to the sterilized slurry, and ferment at 30°C for 48 hours; when the fermentation process is carried out in a fermenter, the ve...

Embodiment 2

[0059] The difference from Example 1 is that no high-pressure homogenization is performed, and the cell membrane is not released.

Embodiment 3

[0061] Microscopic state comparison of oil droplets

[0062] 1. Sample preparation

[0063] Example 1 of the present invention (5 times of high-pressure homogenization) and Example 2 were used as samples to be tested.

[0064] 2. Nile red staining

[0065] Nile red is a fat-soluble dye that can stain the fat droplets in the fermentation broth red. Nile Red does not fluoresce in most polar solvents, however, it fluoresces strongly in lipid-rich environments.

[0066] Nile red dye storage solution: Nile red 10mg, acetone 10mL, fully mixed and stored in the dark.

[0067] Nile Red working solution: 100 μL of Nile Red stock solution, 1 mL of PBS (pH 7.4), mix thoroughly before use.

[0068] Take 1 mL of the sample to be tested, add 1 mL of Nile Red working solution under dark conditions, and incubate for 5 min. Take 10 μL and place it on a glass slide, cover it with a cover glass, and use a laser confocal microscope to scan and observe the fluorescence of the prepared film. ...

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PUM

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Abstract

The invention discloses a preparation method, product and application of a high-stability vegetable oil material fermentation self-encapsulation carrier. The fermented product breaks microbial cells homogeneously under high pressure, releases cell membrane fragments, and uses the cell membrane as a natural surfactant to encapsulate camellia Seed oil does not require additional surfactants. The entrapment system has high stability and will not break the emulsion under centrifugation, high temperature and low temperature. The fermented product is rich in protein, amino acid, polyphenol and polysaccharide. The fermented product is green and safe, has no hemolytic effect, has no adverse reaction to human skin, and is non-irritating. The invention is a novel development and utilization of vegetable oil, which is beneficial to improving the economic application value of vegetable oil in cosmetics.

Description

technical field [0001] The invention belongs to the technical field of skin care products, and in particular relates to a preparation method and product of a highly stable vegetable oil material fermented self-packaging carrier and its application. Background technique [0002] Oil can form a hydrophobic film on the skin surface to make the skin soft, lubricated and glossy; in dry and cold climates, it can inhibit the evaporation of moisture on the skin surface and prevent dry skin; as a solvent for special ingredients, it can promote skin absorption of drugs or effective Active ingredient; imparts softness and shine to hair. [0003] Vegetable oils are widely used in the field of skin care products because of their skin-friendly texture, close to the structure of human sebum membrane, easier absorption by the skin, and better permeability. Vegetable oils commonly used in cosmetics in recent years include olive oil, jojoba oil, camellia seed oil, almond oil, etc. At presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/60A61K8/92A61K8/9728A61K8/99A61Q19/00
CPCA61K8/60A61K8/922A61K8/99A61K2800/72A61K2800/85A61Q19/00A61K8/9728
Inventor 邓楠赵炳天杜养标
Owner RAYTING BIOTECHNOLOGY CO LTD
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