A bovine serum albumin-tea polyphenol-molybdenum nanocomposite and its application

A bovine serum albumin and nanocomposite technology, which is applied in the field of nanomedicine, can solve the problems of complex design and preparation process of nanomaterials, clinical transformation and application increase, etc., and achieves easy large-scale synthesis, simple method, and biocompatibility. Good results

Active Publication Date: 2022-01-07
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, only a handful of nanomaterials have been approved by the US Food and Drug Administration for clinical human studies, partly because the potential toxicity of nanomaterials has been a topic worth discussing
On the other hand, the complex design and preparation process of nanomaterials will also increase the difficulty of clinical transformation and application.

Method used

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  • A bovine serum albumin-tea polyphenol-molybdenum nanocomposite and its application
  • A bovine serum albumin-tea polyphenol-molybdenum nanocomposite and its application
  • A bovine serum albumin-tea polyphenol-molybdenum nanocomposite and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Weigh 0.001g bovine serum albumin and dissolve it in 0.4mL ultrapure water, and mix it evenly by ultrasonic and magnetic stirring;

[0044] (2) Add 0.01mL epigallocatechin gallate aqueous solution (10mg mL -1 ), continue to stir, so that the mixture is fully mixed;

[0045] (3) Add 0.03mL MoCl dropwise 5 Aqueous solution (15mg mL -1 ), continue to stir, so that the mixture is fully mixed;

[0046] (4) Add 0.014 mL of NaOH aqueous solution (1mol L -1 ), continue to stir, and react at room temperature for 30min;

[0047] (5) The reactants were dialyzed, and BEMNCs were obtained after dialyzing with a dialysis bag (molecular weight cut-off: 100kD) for 24 hours. Change the water 4-6 times during dialysis.

[0048] (6) After the dialysis is completed, the obtained product is dried and collected with a vacuum freeze dryer.

[0049] figure 1 It is a transmission electron microscope image of BEM NCs in Example 1 of the present invention. It can be seen from the figu...

Embodiment 2

[0054] In order to prove that the BEM NCs obtained in Example 1 of the present invention have a good effect on inducing the immunogenic cell death of mouse cancer cells (4T1), we measured the release of damage-related molecular patterns of 4T1 cells after incubation with BEM NCs, specifically The test method is as follows:

[0055] (1) Analysis of the transfer of calreticulin to the membrane surface of cancer cells

[0056] 1 mL of 4T1 cells (5×10 5 cells) were inoculated into 1 ml culture dish for 24 h. Then, 1 mL of BEM NCs medium solution was added to the cell culture dish and cultured for another 24 h. Afterwards, the cells were washed 3 times with PBS. 4T1 cells were further incubated with anti-calreticulin antibody at 4°C for 12 hours according to the method described in the calreticulin probe manual. Then, cells were further incubated with Cy5-conjugated secondary antibody for 2 hours. Then, nuclei were treated with DAPI (10 μg mL -1 )dyeing. Finally, the express...

Embodiment 3

[0065] To demonstrate whether the immunogenic cell death process described above could stimulate immature DCs to engulf fractions of stressed and dying tumor cells and become mature DCs, a dendritic cell maturation study was performed. The specific test method is as follows:

[0066] (1) BALB / c mice aged 6-8 weeks were selected, and immature dendritic cells were isolated from bone marrow. Specifically, bone marrow was obtained by flushing femurs and tibias with PBS.

[0067] (2) After the erythrocytes were lysed, the cells were inoculated in a 100mm×20mm cell culture dish, and the cells were cultured with DC cell culture medium, and the medium was changed every two days.

[0068] (3) On day 7, non-adherent as well as loosely adherent cells were collected for further use.

[0069] (4) The residual 4T1 cancer cells incubated with BEM NCs medium solution for 24 hours were placed in the upper compartment of the transwell system, while the dendritic cells were seeded in the lower...

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Abstract

The invention provides a bovine serum albumin-tea polyphenol-molybdenum nanocomposite, which belongs to the field of nanomedicine. The complex is to dissolve bovine serum albumin in water, then add tea polyphenol aqueous solution and molybdenum source aqueous solution drop by drop, and then add sodium hydroxide aqueous solution drop by drop, adopt biomineralization method to react, and obtain bovine serum albumin-tea Polyphenol‑Molybdenum Nanocomposite. The invention also provides the application of the bovine serum albumin-tea polyphenol-molybdenum nanocomposite in the preparation of immunogenic cell death and tumor immunotherapy drugs induced by the treatment of cancer cells. The complex of the invention has good biocompatibility, good dispersibility in water, good ability of inducing immunogenic cell death of mouse cancer cells, and can be effectively applied in tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of nanomedicine, and in particular relates to a bovine serum albumin-tea polyphenol-molybdenum nanocomposite and an application thereof. Background technique [0002] Although scientists have made great efforts in the field of tumor therapy in the past decades, it is still a serious challenge to completely eliminate malignant tumors due to their easy recurrence and metastasis. In recent years, immunotherapy has become a hotspot in cancer treatment, which uses the innate immune system to generate anti-tumor immunity, providing new opportunities for fighting tumor recurrence and metastasis. However, according to preliminary clinical research results, due to the low immunogenicity of tumor tissue, and tumor cells can protect themselves from the attack of the immune system through immune evasion and immunosuppression, which ultimately makes it difficult for the body to generate a sustained and effective immune response, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K36/82A61K31/353A61K33/24A61K47/42A61P35/00
CPCA61K36/82A61K31/353A61K33/24A61K9/5169A61P35/00A61K2300/00
Inventor 姜秀娥甄文瑶贾潇丹
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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