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Marine lactobacillus plantarum SS-128 and application thereof in aquatic product preservation

A SS-128, marine plant technology, applied in the direction of microorganism-based methods, using chemicals to preserve meat/fish, bacteria, etc., can solve the problems of less preservation of aquatic products, fewer separation reports, limited applications, etc., and achieve antibacterial High antibacterial effect, good antibacterial effect, effect of improving quality and safety

Inactive Publication Date: 2021-04-16
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although Lactobacillus plantarum has certain antibacterial properties, it is mostly used in plant products or food, but there are few research reports on the preservation effect of aquatic products; from the perspective of isolation sources, the existing Lactobacillus plantarum is mostly from dairy products and fermented foods. isolated, but less reported from the ocean
[0006] In addition, the current Lactobacillus plantarum generally grows rapidly under high temperature and normal temperature conditions, but grows slowly under refrigerated conditions, which limits its application in refrigerated foods, especially refrigerated aquatic products

Method used

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  • Marine lactobacillus plantarum SS-128 and application thereof in aquatic product preservation
  • Marine lactobacillus plantarum SS-128 and application thereof in aquatic product preservation
  • Marine lactobacillus plantarum SS-128 and application thereof in aquatic product preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Separation and purification of marine plant lactobacillus Lactobacillus plantarum SS-128

[0022] The isolation of Lactobacillus plantarum SS-128 was carried out according to the separation and purification process ( figure 1 ). The brief steps are as follows:

[0023] Under aseptic conditions, the intestines of the blackhead fish picked were fully shredded with sterile scissors, 10 g was placed in 90 mL sterile saline, and after homogenization, a line was drawn on the MRS medium containing 1% CaCO3, 30 Cultivate at ℃ for 48h. Pick the white colonies with calcium-dissolving circles for further separation and purification, and pick single colonies for Gram staining and catalase experiments. Shewanella, Pseudomonas and Acinetobacter were selected as indicator bacteria, and the antibacterial activity of the isolated strains was preliminarily screened by the Oxford cup method. Take 4mL indicator bacteria solution (concentration is about 1×10 6 -10 7 CFU / mL) ...

Embodiment 2

[0027] Example 2 Physiological and biochemical identification of Lactobacillus plantarum SS-128

[0028] The growth curve of the strain was determined by the optical density method, and the strain with strong antibacterial activity was inoculated in the MRS liquid medium according to the inoculation amount of 1%, and cultured statically at 4°C, with the MRS medium as the blank control, every 3 days Measure once, measure the optical density value (OD600) of the culture solution at 600nm respectively, each group has three parallels, and use the OD value to judge the growth of each strain under low temperature conditions. The results showed that the bacteria grew rapidly at 4°C low temperature ( figure 2 ),

[0029] According to the "Bergey's Bacteria Identification Manual", the strain SS-128 was initially identified. The results showed that the strain SS-128 was rod-shaped and Gram staining was positive. The bacteria could utilize maltose, cellobiose, sucrose, inulin, and lactos...

Embodiment 3

[0032] The bacteriostatic activity of embodiment 3 marine plant Lactobacillus Lactobacillus plantarum SS-128 to Shewanella, Aeromonas

[0033] Pick the colony of Lactobacillus plantarum SS-128 and inoculate it in MRS broth, cultivate it overnight at 30°C, centrifuge and filter at low temperature, and then prepare the supernatant with LB broth to contain 9% Lactobacillus plantarum SS-128 without Culture medium of bacterial supernatant. Activate Shewanella and Aeromonas overnight, culture overnight at 30°C, and inoculate 1% of the overnight culture of Shewanella and Aeromonas into the prepared Lactobacillus SS-128 supernatant After cultured at 30°C for 24 hours, the diameter of the inhibition zone was measured, and the medium with the same pH was used as a control. The results showed that Lactobacillus plantarum SS-128 had strong antibacterial activity against aquatic product spoilage bacteria Shewanella and Aeromonas. The diameters of the inhibition zones of the two bacteria ...

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Abstract

The invention discloses marine lactobacillus plantarum SS-128 and application thereof in aquatic product preservation, belonging to the technical field of food preservation. The marine lactobacillus plantarum SS-128 is preserved in China General Microbiological Culture Collection Center with an accession number is CGMCC No. 17003. The lactobacillus plantarum SS-128 is derived from the intestinal tract of marine cold-water fish, grows and propagates rapidly under a low-temperature refrigeration condition, is high in antibacterial ability, and can reduce the volatile basic nitrogen level of aquatic products, inhibit spoilage of the aquatic products and prolong the shelf life of the aquatic products. The marine lactobacillus plantarum SS-128 provided by the invention can be applied to the field of biological preservation of aquatic products.

Description

technical field [0001] The invention belongs to the technical field of food preservation, and in particular relates to marine lactobacillus plantarum and its application in preservation of aquatic products. Background technique [0002] The toxic and side effects of traditional chemical preservatives such as sodium benzoate, sodium nitrite, and sodium diacetate have attracted increasing attention. Therefore, looking for safe and non-toxic biological preservatives and biological control technology has become a hot spot of concern. Lactobacillus plantarum widely exists in the human intestine and food, and plays a key role in the production of fermented food and intestinal probiotics. Further studies in recent years have shown that Lactobacillus plantarum not only has fermentation and probiotic effects, but also has good antiseptic properties. Studies have shown that the organic acids produced by Lactobacillus plantarum can inhibit the growth of Listeria monocytogenes and Sal...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23B4/22C12R1/25
Inventor 刘尊英史国萃钱怡霖曾名湧
Owner OCEAN UNIV OF CHINA
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