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Preparation and application of a hadscs-loaded bilayer skin biomimetic hydrogel composite scaffold

A composite scaffold and hydrogel technology, which can be applied to inactive components of polymer compounds, bone/connective tissue cells, prostheses, etc., can solve problems such as single function, achieve good air permeability, promote the formation of blood vessels, and prevent tissue fluid. lost effect

Active Publication Date: 2022-03-04
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to aim at the wound dressing in the prior art, which has a single function, and has biocompatibility, degradability, cell adhesion, load capacity, air permeability, fit to the wound, nutrient transport, wound Deficiencies and deficiencies in healing, providing a biomimetic hydrogel composite scaffold loaded with hADSCs bilayer skin

Method used

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  • Preparation and application of a hadscs-loaded bilayer skin biomimetic hydrogel composite scaffold
  • Preparation and application of a hadscs-loaded bilayer skin biomimetic hydrogel composite scaffold
  • Preparation and application of a hadscs-loaded bilayer skin biomimetic hydrogel composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Loading hADSCs bilayer skin biomimetic hydrogel composite scaffold

[0069] 1. Preparation of gelatin electrospun fiber membrane

[0070] Preparation of electrospinning solution: Weigh 0.5 g of gelatin with a microelectronic balance and dissolve in 5 mL of hexafluoroisopropanol (HFIP) in a glass bottle (10 mL), add a magnetic stirrer, place on a magnetic stirrer, and magnetically stir for 24 hours. use.

[0071] Electrospinning method: the electrospinning experiment is carried out at room temperature and humidity below 50%. The positive electrode high-pressure injection head adopts 8# flat needle (length 20cm), the voltage regulation is 16kV, the distance from the needle to the receiving device is 20cm, the propulsion speed is 16.7μL / min, and the volume of the electrospinning solution is 320μL. Spread aluminum foil paper on a self-assembled platform with a size of 10cm×10cm, and place several 15mm glass slides for sample collection.

[0072] During the expe...

Embodiment 2

[0096] Example 2 Bilayer Skin Biomimetic Hydrogel Composite Scaffold Loaded with hADSCs

[0097] 1. The appearance of the prepared hADSCs-loaded bilayer skin structure hydrogel composite scaffold is as follows: figure 1 shown.

[0098] from figure 1 It can be seen that the prepared double-layer skin structure hydrogel composite scaffold loaded with hADSCs is hydrogel-like, in which the gelatin electrospun fiber membrane is the base layer, and the cylindrical gelatin hydrogel is evenly arranged on it, and the cylindrical gelatin hydrogel Keep a certain interval between the hydrogels.

[0099] The overall thickness of the composite scaffold prepared in Example 1 is 350 μm; the diameter of the cylindrical hydrogel is 600 μm; the height is 300 μm.

[0100] 2. Detection of the cell loading of the hADSCs-loaded bilayer skin biomimetic hydrogel composite scaffold

[0101] Taking the gelatin electrospun fiber membrane prepared in 1 as a comparison, compare its influence with the c...

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Abstract

The invention discloses the preparation and application of a double-layer skin biomimetic hydrogel composite scaffold loaded with hADSCs. The composite scaffold is based on gelatin electrospun fiber membrane, adipocyte stem cell suspension is added for adhesion, and then gelatin photocrosslinking solution is added to prepare cylindrical gelatin hydrogel through photocrosslinking, which is the Composite bracket. The composite scaffold has a double-layer structure, in which the fibrous membrane can block the invasion of foreign bodies and pathogens, and prevent the loss of interstitial fluid; the cylindrical gelatin not only ensures the loading capacity, viability and adhesion of hADSCs, but also promotes the migration and proliferation of hADSCs, synergistically Promote the regeneration and formation of skin tissue; and its unique shape, while ensuring a good fit between the composite scaffold and the wound, it also has good air permeability, which is conducive to the transportation of oxygen and nutrients in the wound, and can also promote wound healing. The generation and degradation cycle of blood vessels, new granulation tissue and collagen is close to the wound healing cycle, which is beneficial to wound healing.

Description

technical field [0001] The invention relates to the technical field of biological tissue scaffolds, in particular to the preparation and application of a double-layered skin biomimetic hydrogel composite scaffold loaded with hADSCs. Background technique [0002] As the most important barrier to the human body, the skin often suffers from acute and chronic injuries, especially difficult-to-heal wounds, which seriously affect the quality of life of patients. For these refractory wounds that cannot be healed by various surgical methods, stem cell transplantation has become an effective research direction. As one of adult stem cells, adipose stem cells play an indispensable role in repairing skin wounds than other stem cells because of their immunocompatibility advantages. Based on a large number of animal and clinical trials, adipose stem cells will have a greater breakthrough in the field of skin wound repair in the future, especially in the field of chronic refractory wounds...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/38A61L27/52A61L27/58A61L27/54A61L27/60A61K9/06A61K47/42A61L26/00C12N5/0775
Inventor 李燕臧宏运宋婷
Owner SUN YAT SEN UNIV