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Paecilomyces lilacinus microcapsule suspending agent and preparation method thereof

A technology of Paecilomyces lilacinus and microcapsule suspension, applied in botany equipment and methods, biocide, biocide, etc., can solve the problems of low encapsulation rate of microcapsules, high toxicity, poor capsule stability, etc. , to achieve the effect of improved control effect, stable performance and good compatibility

Active Publication Date: 2021-04-27
北京启高生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The encapsulation materials used in the interfacial polymerization method are highly toxic and more suitable for non-biological materials; the capsule wall materials used in the complex coacervation method are gelatin, gum arabic, sodium alginate, etc., which are naturally non-toxic and have good biocompatibility. However, the encapsulation efficiency of the microcapsule is not high, and the stability of the capsule is poor.

Method used

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  • Paecilomyces lilacinus microcapsule suspending agent and preparation method thereof
  • Paecilomyces lilacinus microcapsule suspending agent and preparation method thereof
  • Paecilomyces lilacinus microcapsule suspending agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Preparation of Paecilomyces lilacinus fermented bacterial liquid by liquid submerged fermentation

[0044] Preparation of fermentation medium: weigh 10 grams of sucrose, 20 grams of yeast powder, 4 g of peptone, MgSO 4 ·7H 2 O 0.5 g, KH 2 PO 4 0.5 grams, 5 grams of bean cake powder, add water to 1000 ml, adjust the pH to 5.6 with hydrochloric acid, and sterilize to become the fermentation medium. Amplified according to this formula is the culture medium for submerged fermentation in the fermenter. The medium volume of this embodiment is 5000L.

[0045] Get the seed culture in the middle and late stages of logarithmic growth, transfer it to a fermenter equipped with a fermentation medium with an inoculum size of 8% (V / V), keep the tank temperature at 25°C, and the tank pressure at 0.5kg / cm, stir The speed is 150 rpm, the ventilation rate is 1:0.6, and the fermentation and cultivation time is 60 hours.

[0046] The bacteria content in the fermentation...

Embodiment 2

[0047] Embodiment 2 The preparation of Paecilomyces lilacinus microcapsule suspension

[0048] The Paecilomyces lilacinus microcapsule suspension proposed in this embodiment is composed of ingredients for forming an oil phase, ingredients for forming a water phase, and capsule wall materials; the ingredients for forming an oil phase include Paecilomyces lilacinus Mold concentrated bacteria liquid and auxiliary agent; the ingredients used to form the water phase include emulsifier, Paecilomyces lilacinus supernatant; the capsule wall material is polymerized by the capsule wall material prepolymer, and the capsule wall The material prepolymer includes isocyanates and organic amines, and the auxiliary agents include acid-base regulators, dispersants, and solvent oils; the concentrated Paecilomyces lilacinus liquid and supernatant are isolated Paecilomyces lilacinus fermented liquid owned.

[0049] The preparation method of described Paecilomyces lilacinus microcapsule suspension...

Embodiment 3

[0059] Embodiment 3: liquid submerged fermentation prepares Paecilomyces lilacinus fermented bacterial liquid

[0060] The preparation of the fermentation medium is the same as in Example 1.

[0061] Take the seed culture in the middle and late stages of logarithmic growth (the culture method is the same as that in Examples 1 and 2), transfer it into a fermenter with a fermentation medium at an inoculum size of 8% (V / V), and keep the tank temperature at 25 °C, tank pressure 0.5kg / cm, stirring rate 150rpm, ventilation rate 1:0.6, fermentation time 72 hours.

[0062] The bacteria content in the fermentation broth was 3 billion / mL.

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Abstract

The invention provides a paecilomyces lilacinus microcapsule suspending agent. The paecilomyces lilacinus microcapsule suspending agent is prepared from components for forming an oil phase, components for forming a water phase and a capsule wall material, wherein the components for forming the oil phase comprise a paecilomyces lilacinus concentrated bacterial solution and an auxiliary agent; wherein the components for forming the water phase comprise an emulsifier and paecilomyces lilacinus supernatant, the capsule wall material is formed by polymerizing a capsule wall material prepolymer, and the capsule wall material prepolymer comprises isocyanate and organic amine. The auxiliary agent comprises one or more of an acid-base regulator, a dispersing agent, a solvent oil, an anti-freezing agent, a defoaming agent, a preservative and a viscosity regulator; the paecilomyces lilacinus concentrated bacterial solution and the supernatant are obtained by separating paecilomyces lilacinus fermentation bacterial liquid. According to the paecilomyces lilacinus microcapsule suspending agent provided by the invention, the water phase and the oil phase respectively contain metabolite and effective viable bacteria of paecilomyces lilacinus, and compared with conventional powder, granules and water dispersants, the microcapsule suspending agent is longer in shelf life and more stable in performance.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum, in particular to a Paecilomyces lilacinus biological inoculum and a preparation method thereof. Background technique [0002] Paecilomyces lilacinus ( Paecilomyces lilacinus (Thorn.) Samson) is a fungus of the Hyphospora family and the genus Paecilomyces, which belongs to the parasitic fungus, and its secretion and metabolism produce a variety of organic acids, enzymes, and physiologically active substances. Paecilomyces lilacinus can parasitize eggs, and can also infect larvae and females. After the spores of Paecilomyces lilacinus germinate, the hyphae produced can penetrate the egg shells, larvae, and female adult body walls of nematodes. The nematode absorbs nutrients and reproduces, destroying the normal physiological metabolism of eggs, larvae and female adults, resulting in the death of plant nematodes. [0003] Paecilomyces lilacinus can not only inhibit nematode infection, b...

Claims

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Application Information

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IPC IPC(8): A01N63/36A01N25/28A01N25/04A01P5/00A01P21/00C05F11/08C05G5/20
CPCA01N25/04A01N25/28A01N63/36C05F11/08C05G5/20
Inventor 钟增明
Owner 北京启高生物科技有限公司
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