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Bacillus catonella strain DM4-2 with inhibiting effect on plant scab as well as fungicide and application thereof

A technology of Bacillus carinii and scab, applied in the field of microorganisms, can solve problems such as unachieved results and the complexity of plant resistance physiological mechanisms

Active Publication Date: 2021-05-11
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researchers hope that the occurrence of scab can be prevented by breeding disease-resistant varieties, but due to the complexity of genetic and physiological mechanisms of plant resistance, ideal results have not been obtained.

Method used

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  • Bacillus catonella strain DM4-2 with inhibiting effect on plant scab as well as fungicide and application thereof
  • Bacillus catonella strain DM4-2 with inhibiting effect on plant scab as well as fungicide and application thereof
  • Bacillus catonella strain DM4-2 with inhibiting effect on plant scab as well as fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and purification of Bacillus cabrialesii DM4-2

[0035] Add 1 g of soil samples derived from oceanic sediments into a sealed glass bottle (filled with 1 / 50 concentration of 2216E medium for enrichment culture), and carry out oligotrophic recovery culture in an incubator at 25°C (or a similar temperature), shaking for one day twice. Then on the 2nd, 10th and 30th day, draw 1mL of culture solution into 10mL sterile water and mix well to make 10 -1 Diluent, draw 1mL10 -1 Add the diluent to 9mL sterile water and mix well to make 10 -2 diluent, and so on to make 10 -3 、10 -4 、10 -5 Diluent, take 10 -3 、10 -4 、10 -5The diluted solution was spread on 1 / 2 concentration 2216E solid medium, and cultured at 28°C.

[0036] Select a single colony with different morphological characteristics for purification, and inoculate the single bacterial colony in 2216E liquid medium, shake and culture at 28°C and 180rpm for 24-72h, and spread potato scab evenly...

Embodiment 2

[0037] Example 2: Identification of Bacillus cabrialesii DM4-2

[0038] 1. Morphological and physiological and biochemical characteristics of the strain

[0039] The colony morphology of bacterial strain DM4-2 on the LB solid plate is as follows: figure 1 Shown is round, milky white, opaque, indicating smooth moist, sticky texture, with irregular edges. Take the strains in the stable phase for Gram staining, such as figure 2 The lavender shown is a Gram-positive bacterium, all of which are oval in shape when observed under an optical microscope. The growth temperature of the strain DM4-2 is 28-37°C, the pH is 5-12, the optimum growth temperature is 28°C, and the optimum pH is 7.

[0040] 2. 16S rDNA identification of the strain:

[0041] Genome extraction kit was used to extract the genome of bacterial strain DM4-2, using it as a template, 16S rDNA universal primer 27F (5'-GAG AGT TTG ATC CTG GCT CAG-3') and 1492R (5'-ACG GAT ACC TTG TTA CGA CTT-3') primers were used to ...

Embodiment 3

[0044] Embodiment 3: the antagonistic effect of Bacillus cabrialesii DM4-2 cultured in different mediums

[0045] Inoculate the purified bacterial DM4-2 single colony in 2216E liquid medium for 24-72h under the conditions of 28°C and 180rpm, and then centrifuge at 12000rpm for 3min to obtain the supernatant, and then use a sterile Resuspend in water to obtain the bacterial suspension, and carry out antagonism experiment together with the fermentation broth (i.e. evenly spread 200 μL of potato scab pathogen S. Insert 100 μL of fermentation broth, supernatant, bacterial suspension, and 2216E liquid medium into the wells, culture at 28°C for 5 to 7 days, and judge the source of antibacterial substances by the presence or absence of antibacterial zones and the size of the antibacterial zones).

[0046] Experimental results such as image 3 As shown, the fermentation broth of the bacterial strain DM4-2 of the present invention has a better antagonistic effect. Using the same cult...

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Abstract

The invention provides a Bacillus catonella strain DM4-2 with inhibiting effect on plant scab as well as a fungicide and an application thereof, the strain DM4-2 is preserved in China Center for Type Culture Collection on January 7, 2021, and the preservation number is CCTCC M 2021026. According to the invention, an antagonism experiment proves that the strain has a relatively strong inhibition effect on Streptomyces scabies. The invention provides an application of the fermentation liquor of the strain in preventing and treating potato scab. Experiments prove that the strain can significantly improve various physiological indexes such as root activity, antioxidant enzyme system and the like of potato plants, and also significantly improve the activity of soil urease, thereby the nitrogen supply capacity of soil is improved. According to the present invention, the Bacillus catonella DM4-2 has strong inhibition effect on S.scabies, which can be prepared into the biocontrol agent for preventing and treating potato scab, and has important market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Bacillus carinii DM4-2 capable of preventing and treating plant scab, its agent and application. Background technique [0002] Potatoes are the fourth largest food crop in the world. According to incomplete statistics from the Food and Agriculture Organization of the United Nations (FAO), by 2018, its output had increased to 368 million tons. Among the common diseases of potatoes, potato scab is a soil-borne disease mainly caused by S.scabies, S.acidiscabies, S.turgidiscabies, etc. The pathogenic bacteria can be found in soil sediments and spread by wind and water flow or disease species Tubers spread. The phytotoxins produced by them can destroy the cortex and epidermal tissue of potato tubers, thereby causing scab lesions on potato tubers, and the quality of tubers caused by scab is rejected by consumers and manufacturers. As a global disease, scab has caused h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01G13/00A01G22/25C12R1/07
CPCC12N1/20A01N63/22A01G13/00A01G22/25
Inventor 高峥苏高雅赵振懿王永东史文聪周波
Owner SHANDONG AGRICULTURAL UNIVERSITY
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