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Disease-resistant and quality-improving microbial agent special for tobacco and application of microbial agent

A microbial inoculum, Arthrobacter nicotianae technology, applied in the direction of microorganisms, microbial treatment, microorganism-based methods, etc., can solve problems such as unsuitable long-term use, soil health problems, etc.

Pending Publication Date: 2021-05-14
FUJIAN SANJU BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN110506598A discloses a comprehensive prevention and control method of tobacco root black rot, using Bacillus subtilis and chemical agents to jointly treat, the control effect reaches 88.36%, but this method is not suitable for long-term use, and it is easy to cause soil health problems; A Trichoderma viride with disease prevention and growth promotion function and its application, the control effect on tobacco root rot is over 65%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Microbial agents

[0033] The functional bacteria are respectively fermented through the Erlenmeyer flask→seed tank→fermentation tank to obtain a certain concentration of Arthrobacter nicotianae, Paenibacillus jellylike and Paenibacillus cribs.

[0034] Arthrobacter tobacco shake flask medium is beef extract peptone medium; seed tank and fermenter medium: tryptone 0.1%, yeast powder 0.05%, sodium chloride 0.15%, the rest is water, pH 6.5-7.0. The fermentation conditions were 28°C, 200r / min for 36h. The Arthrobacter tobacco concentration at the end of fermentation was 1 billion / mL.

[0035] Paenibacillus jelly-like shake flask medium is nitrogen-free medium; seed tank and fermentation medium: yeast extract 0.15%, starch 0.5%, soybean meal powder 1.5%, magnesium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, carbonic acid Calcium 8%, sodium chloride 0.02%, the rest is water, pH 7.2-7.4. The fermentation conditions were 30°C, 180r / min for 48h. The concentration of ...

Embodiment 2

[0039] Microbial agents

[0040] The culture medium in Example 1 was used to ferment the functional bacteria respectively to obtain certain concentrations of Arthrobacter nicotianae, Paenibacillus jelly-like and Paenibacillus cribs.

[0041] The fermentation conditions of Arthrobacter tobacco fermenter culture were 28°C, 200r / min for 24h. The concentration of Arthrobacter tobacco at the end of fermentation was 1.10 billion / mL.

[0042] The fermentation condition of the jelly-like Paenibacillus fermenter was 35° C. and 200 r / min for 72 hours. The concentration of Paenibacillus jelly-like at the end of fermentation was 2.30 billion / mL.

[0043] The fermentation condition of Paenibacillus fermenter cultured by Cribb is 28°C, 180r / min for 36h. The concentration of Paenibacillus spp. at the end of fermentation was 1.60 billion / mL.

[0044] Mix the fermentation liquid of Arthrobacter nicotianae, Paenibacillus jelly-like and Paenibacillus crib evenly according to the volume ratio...

Embodiment 3

[0046] Microbial agents

[0047] The culture medium in Example 1 was used to ferment the functional bacteria respectively to obtain certain concentrations of Arthrobacter nicotianae, Paenibacillus jelly-like and Paenibacillus cribs.

[0048] The fermentation condition of the Arthrobacter tobacco fermenter was 28°C, 200r / min for 36h. The concentration of Arthrobacter tobacco at the end of fermentation was 1.80 billion / mL.

[0049] The fermentation condition of the jelly-like Paenibacillus fermenter was 37° C., 190 r / min for 36 hours. The concentration of Paenibacillus jelly-like at the end of fermentation was 1.50 billion / mL.

[0050] The fermentation condition of Paenibacillus fermenter cultured by Cribb is 32°C, 190r / min for 24h. The concentration of Paenibacillus sp. at the end of fermentation was 800 million / mL.

[0051] The fermentation liquid of Arthrobacter nicotianae, Paenibacillus jelly-like and Paenibacillus cribbus are uniformly mixed according to the volume rati...

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Abstract

The invention provides a disease-resistant and quality-improving microbial agent special for tobacco and application of the microbial agent, and belongs to the technical field of biological control. The microbial agent is mainly prepared by mixing arthrobacter nicotianae fermentation liquor, paenibacillus mucilaginosus fermentation liquor and paenibacillus kribrueckii fermentation liquor, the bacterium concentration of the arthrobacter nicotianae fermentation liquor is larger than or equal to 10.0 billion / mL, the bacterium concentration of the paenibacillus mucilaginosus fermentation liquor is larger than or equal to 10.0 billion / mL, and the bacterium concentration of the paenibacillus kribrueckii fermentation liquor is larger than or equal to 5.0 billion / mL. The mixing volume ratio of the arthrobacter nicotianae fermentation liquor to the paenibacillus mucilaginosus fermentation liquor to the paenibacillus kribrueckii fermentation liquor is 1: (1-2): (1-3). The control effect of the fertilizer containing the microbial agent on tobacco root rot reaches 89.73%, and the tobacco quality can be remarkably improved by compounding the three bacteria.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a special tobacco bacterial agent for disease resistance and quality improvement. Background technique [0002] Tobacco is one of the main economic crops in our country and plays an important role in the development of the national economy. Tobacco has a long growth cycle and is vulnerable to pests and diseases, which restricts the development of the tobacco industry and causes major economic losses. In my country, it is mainly leaf and root diseases, among which tobacco damping-off, blight, bacterial wilt, black shank and root rot are the main root diseases. There are many pathogenic bacteria that cause root rot, but most of them are reported as Fusarium. Tobacco Fusarium root rot is a relatively harmful disease in the seedling stage of tobacco. can happen. [0003] At present, the most direct and effective measure to prevent and control tobacco root rot is chemica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60C05G5/12C05F17/20C05F17/50A01G22/45A01G13/00C12R1/06C12R1/01
CPCC12N1/20C05F5/00C05F17/20C05F17/50C05G3/60C05G5/12A01G22/45A01G13/00C05F11/00C05F11/08Y02W30/40
Inventor 李建华危月辉姚健孙晓伟王京刘玉珍郭小红法鹏飞
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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