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Chlorin e6-nanogold compound for photothermal and photodynamic therapy of tumors and preparation method of chlorin e6-nanogold compound

A photodynamic therapy and photothermal synergy technology, applied in the fields of chemical synthesis and biomedicine, can solve the problems of low uptake rate of photosensitizers and gold nanoparticles, and achieve the effects of enhanced photodynamic efficacy, strong photostability, and mild reaction conditions.

Inactive Publication Date: 2021-05-21
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention uses hollow spike-like nano-gold as a carrier, and loads a certain proportion of chlorin e6 photosensitizer, and designs a nanocomposite with both near-infrared absorption and photosensitization functions to solve the problem of photosensitizer and nano Scientific issues of low uptake rate of gold in tumor cells or tissues

Method used

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  • Chlorin e6-nanogold compound for photothermal and photodynamic therapy of tumors and preparation method of chlorin e6-nanogold compound
  • Chlorin e6-nanogold compound for photothermal and photodynamic therapy of tumors and preparation method of chlorin e6-nanogold compound
  • Chlorin e6-nanogold compound for photothermal and photodynamic therapy of tumors and preparation method of chlorin e6-nanogold compound

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Preparation and Characterization of Hollow Spike-shaped Gold Nanoparticles: Prepared by Displacement Reaction of Chlorauric Acid and Silver Nanoparticles. Concrete steps: (1) Silver nanoparticles with a particle size of about 30nm are prepared by sodium citrate reduction method: take newly prepared 180 μL of 1% AgNO 3 Add the solution into 25mL of deionized water, stir and boil, then quickly add 5mL of 1% sodium citrate solution, keep boiling for 30min, and cool to room temperature; (2) After centrifuging and washing 5mL of silver nanoparticles, resuspend and disperse in 4mL of water and slowly add HAuCl dropwise 4 Solution (140 μL 1% HAuCl 4 Add the solution to 1mL of ultrapure water to dilute it), shake and mix well, and the color of the solution gradually changes from bright yellow to light gray. Immediately after that, 1 mL of 10 mM ascorbic acid solution was added, mixed rapidly, and allowed to stand to obtain a hollow spike-shaped gold nano-stock solution with a...

Embodiment 2

[0039] In order to compare the advantages of the designed nanocomposites, gold nanoparticles (GNS for short) modified only with LA-PEG-OMe were selected as a negative control. The specific preparation method is basically the same as in Example 1. Specifically, take 2mL of the newly prepared nano-gold stock solution, add 180uL of LA-PEG-OMe storage solution, shake and react at room temperature overnight, centrifuge to remove the supernatant, and resuspend in ultrapure water , that is.

Embodiment 3

[0041] GNS-Ce6 tumor cell uptake fluorescent imaging test: Inoculate KB oral cancer cells on a special confocal culture plate, incubate in a 37°C incubator for 24 hours, remove the original medium, wash twice with PBS, add GNS (50 μg / mL ), GNS-Ce6 (50 μg / mL) and the same amount of Ce6 (0.6 μg / mL) in DMEM cell culture medium, incubated for 24 hours, discarded the original medium, and washed twice with PBS. Add an appropriate amount of Hoechst 33342 nuclear staining solution to fully cover the adherent cells, incubate in a 37°C incubator for 20 minutes, discard the staining solution, and wash with PBS 2-3 times for confocal fluorescence imaging and bright field imaging detection. Imaging results such as Figure 4 As shown, the nuclei show blue fluorescence, and Ce6 shows red fluorescence. Fluorescent imaging showed that compared with the Ce6-treated group, cells in the GNS-Ce6 group showed a stronger red fluorescence signal, which indicated that Ce6 loading on the surface of GN...

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Abstract

The invention discloses a chlorin e6-nanogold compound for photothermal and photodynamic therapy of tumors and a preparation method of the chlorin e6-nanogold compound. The preparation method specifically comprises the following steps: preparing hollow spike-shaped nanogold through a replacement reaction of a chloroauric acid compound and silver nanoparticles; synthesizing a chlorin e6 functional molecule cross-linked with polyethylene glycol at the tail end of lipoic acid; and using the prepared nanogold as a carrier, and simultaneously compounding a certain proportion of lipoic acid polyethylene glycol methoxyl and LA-PEG-Ce6 on the surface of the nanogold, so that the chlorin e6-nanogold compound with dual functions of near infrared absorption and photosensitization is constructed. The compound has the advantages of low cost, simple preparation method, high stability, good biocompatibility and the like. A tumor cell experiment further proves that the GNS-Ce6 compound has a remarkable effect on improving the uptake efficiency of the chlorin e6 and the nanogold. The survival rate of cancer cells can be effectively reduced through mediated photothermal and photodynamic therapy.

Description

technical field [0001] The invention relates to the fields of chemical synthesis and biomedicine, in particular to a chlorin e6-nano-gold complex for photothermal synergistic photodynamic therapy of tumors and a preparation method thereof. Background technique [0002] Cancer is one of the major diseases that endanger human health, and has been paid more and more attention by scientific research and medical workers. The traditional treatment methods for malignant tumors mainly include chemotherapy, radiotherapy and surgery. At present, combining the advantages and disadvantages of various treatment methods, more interdisciplinary disciplines have begun to pay attention to the development and utilization of new diagnosis and treatment strategies. [0003] Photodynamic therapy (PDT), as a promising new treatment method, is mostly used in the treatment of cancer and other malignant diseases. It mainly consists of three elements: light source, tissue oxygen and photosensitizer....

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/60A61P35/00C08G65/338
CPCA61K41/0052A61K41/0071A61K47/60A61P35/00C08G65/338
Inventor 马宁宁马超
Owner XUZHOU NORMAL UNIVERSITY
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