Anaerobic microorganism culture medium deoxygenization device capable of adjusting gas composition and deoxygenization method

Abstract

The invention belongs to the technical field of anaerobic microorganism culture medium deoxygenization devices, and particularly provides an anaerobic microorganism culture medium deoxygenization device capable of adjusting gas composition, which comprises a controller, a main gas circuit and a branch gas circuit, and is characterized in that the main gas circuit starts from a gas outlet communicated with the outside; a vacuum system, a valve system I, an air pressure detection system I, a valve system II, a valve system III, an air pressure detection system II and a gas storage system are sequentially connected. Meanwhile, the invention discloses an automatic anaerobic microorganism culture medium preparation device which comprises a main gas circuit, a box body and a controller, a supporting ring is detachably connected to the upper surface of the box body, a bottom plate is fixedly connected to the lower surface of the interior of the box body, and the main gas circuit is placed in the bottom plate; the right side of the main gas path is sequentially communicated with a vacuum meter, a first electromagnetic valve and a vacuum pump from front to back, gas is stored in the storage device, the storage device can be used for providing special gas needed by microorganisms, and microorganism preparation is facilitated.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation. The invention specifically relates to an anaerobic microorganism culture medium deoxygenation device and a culture medium automatic deoxygenation method with adjustable gas composition. Background technique [0002] Microorganisms have been involved in all aspects of human production and life. For example, our fermented food processing requires microorganisms, biopharmaceuticals require microorganisms, maintain human health require microorganisms, and sewage treatment requires microorganisms. According to the requirements of microorganisms for the oxygen content in the growth environment, they can be divided into aerobic microorganisms, facultative anaerobic microorganisms and anaerobic microorganisms. Because surface air contains oxygen, most microorganisms are aerobic or facultative anaerobes. However, microorganisms in human or animal intestines, deep-sea microorganisms, an...

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Problems solved by technology

These methods have shortcomings such as high technical requirements, complicated operation, and inability to monitor the growth of microorganisms in real time. For example, the commonly used anaerobic cylinder method uses a common drying cylinder, and then uses physical and chemical methods to create an anaerobic environment in the cylinder. Facilitate the observation, sampling and detection of the growth process of anaerobic microorganisms; the anaerobic bag method is to install a gas generating tube (sodium bicarbonate solid with sodium borohydride and 5% citric acid ampoule) in a plastic bag, a methylene blue indicator tube, Palladium catalyst tube, desiccant, etc., through complex operations to create an anaerobic environment in the plastic bag, this method is costly, takes a long time to establish an anaerobic environment, and is as inconvenient as the anaerobic cylinder method for observation and sampling during the growth of anaerobic microorganisms and detection; the anaerobic glove box is a large airtight metal box. Before operation, the experimental materials that are about to enter the box must be degassed for a long time and filled with nitrogen in the channel connected to the anaerobic glove box. Although the oxygen glove box method can provide a stable anaerobic environment and can observe and operate in real time during the growth of anaerobic microorganisms, the price of the anaerobic glove box is expensive, and the preparation before the experiment is also very complicated; Realize the observation and operation during the growth of microorganisms. In addition, the anaerobic environment of the anaerobic box is unstable; most of the biological aerobic methods are to put plants in airtight containers to consume oxygen and generate carbon dioxide at the same time. The experimental operation is difficult and time-consuming. It is not conducive to the observation, sampling, qualitative and quantitative detection during the growth of anaerobic microorganisms; although the flat-plate pyrogallic acid method and the high-layer agar culture method are low in cost and relatively simple in operation, the cultivation effect of anaerobic microorganisms is not ideal. The scope of application of the method is also narrow, and the sampling and separation effects of anaerobic microorganisms are difficult to control; the Hengate anaerobic rolling tube technology closest to the present invention is the most effective technology for cultivating strict and obligate anaerobic microorganisms at present. However, the process is complicated and the operation is cumbersome, and it is impossible to make anaerobic culture tubes in batches. It can only be used to make anaerobic culture tubes now, and it cannot achieve the effect of ready-to-use.

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