Chitosan nano rhodium material and application thereof

A chitosan nano and nano rhodium technology, applied in the field of biomedicine, can solve the problems of high mortality, toxic side effects, and high cost of drug-resistant bacterial infection, and achieve increased biocompatibility, high affinity, and huge application potential. Effect

Active Publication Date: 2021-06-01
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the occurrence of drug-resistant bacterial infection, we can only be forced to choose second-line or third-line antibiotics. Usually, these antibiotics have good therapeutic effects, but have greater toxic side effects, and are more expensive, and even some super-resistant bacteria are There are no antibiotics to resist, and drug-resistant bacterial infections still have a high mortality rate, so the development of new antibacterial drugs is imminent

Method used

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  • Chitosan nano rhodium material and application thereof
  • Chitosan nano rhodium material and application thereof
  • Chitosan nano rhodium material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1 chitosan nano-rhodium comprises the following steps:

[0050] 1. Synthesis of nano-silver triangles

[0051] (1) Add 250μL of 10mM AgNO to a 50mL glass bottle 3 , 300 μL 30mM sodium citrate dihydrate (sodium citrate), 1.5mL 3.5mM PVP, 24.75mL deionized water and stir well;

[0052] (2) Add 60 μL of hydrogen peroxide (30%) to (1) under magnetic stirring at 300 rpm and incubate for 3 minutes;

[0053] (3) Add 250 μL of 100 mM sodium borohydride solution to (2) and incubate for 3 hours, the color changes from transparent to light yellow, dark yellow, orange, and finally blue;

[0054] (4) Prepare growth solution: add 20mL 1mM AgNO3 to a 25mL round bottom flask; 0.125mL 100mM citric acid, 10μL 75mM sodium citrate dihydrate;

[0055] (5) Add 13 mL of growth solution to (3) at 0.2 mL / s, and the color of the solution turns dark blue. After reacting for 10 minutes, measure the ultraviolet characterization.

[0056] Two, the synthesis of nano...

Embodiment 2

[0073] The antibacterial function of embodiment 2 nanometer rhodium

[0074] The bacteria used in the antibacterial experiment were methicillin-resistant Staphylococcus aureus (MRSA), and the experimental process was as follows:

[0075] (1) Nano-rhodium antibacterial experiment:

[0076] ① Dilute 1.6 equivalents of nano-rhodium gradients with the LB solution of MRSA (original OD 600 =0.5) 1:1 mixing;

[0077] ②Check OD every hour 600 Value, recorded for 12 hours.

[0078] Such as Figure 4 Shown in A, when the concentration of nanometer rhodium is greater than or equal to 0.4 equivalent, there is better antibacterial effect;

[0079] (2) Nano-rhodium sterilization experiment

[0080] ① 0.4 equivalent of nano-rhodium and MRSA (1 × 10 7 CFU) in vitro for 2 hours at 37°C;

[0081] ② Gradiently dilute the ① incubation solution by 10 4 times, take 100 microliters of dilutions in each order of magnitude to coat the plate, and incubate at 37°C for 24 h;

[0082] ③ Take eac...

Embodiment 3

[0084] Embodiment 3 chitosan nano-rhodium is to bacterial affinity under different pH

[0085] The pH value of the microenvironment at the site of bacterial infection is slightly decreased (pH 6.3) due to the influence of bacterial metabolites.

[0086] GCS is a water-soluble biopolymer with a pH-responsive charge. The positive charge value of chitosan nano-rhodium increases with the decrease of pH, which means that chitosan nano-rhodium can increase its positive charge in a slightly acidic environment. Improves affinity for negatively charged bacteria. When MRSA (drug-resistant Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria) were incubated with chitosan nano-rhodium under the condition of pH 6.3, the negative charge on the surface of the bacteria was converted into a positive charge ( Figure 5 A), shows that chitosan nano-rhodium can adhere well on the bacterial surface and has a high affinity. Further, bacteria coating experiments confirmed that chit...

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Abstract

The invention belongs to the field of biological medicine, and particularly discloses chitosan nano rhodium, which is obtained by the following method: S1, coating nano rhodium with polydopamine: adding nano rhodium into a dopamine hydrochloride alkaline solution, and carrying out ultrasonic treatment; and S2, adding the polydopamine nano-rhodium in the step S1 into an ethylene glycol-containing chitosan solution, and coating the surface of the polydopamine nano-rhodium with a layer of chitosan through a Schiff base reaction to form the chitosan nano-rhodium. The chitosan nano rhodium prepared by the invention is simple to synthesize and high in biocompatibility, has a very good antibacterial effect, can be applied to the fields of disinfection and sterilization, due to the fact that thethe pH response function of the chitosan nano rhodium has high affinity to bacteria in a weak acid environment (an infected part), the local infection can be resisted, and the material has huge application potential in infectious skin diseases such as infection aftersubcutaneous abscess,trauma and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a novel nano-metal antibacterial agent and its application, more specifically, to a chitosan nano-rhodium and its application. Background technique [0002] Pathogenic bacterial infection is the second leading cause of death in the world. Antibiotic therapy is by far the most conventional method to treat bacterial infection. Its principle is to inhibit the growth of bacteria or directly kill bacteria through antibiotics to achieve the treatment of bacterial infection the goal of. However, in the past few decades, the uncontrolled use of antibiotics has brought opportunities for bacteria to evolve, and the continuous emergence of various new drug-resistant strains has also made conventional antibiotic treatment fail. For example, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococcus (VRE), penicillin-resistant Streptococcus pneumoniae (PRSP), etc. Today, d...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K31/722A61K9/51A61K47/36A61P31/04A61P17/02
CPCA61K33/24A61K31/722A61K9/5161A61K9/5192A61P31/04A61P17/02A61K2300/00Y02A50/30
Inventor 黄曦谭青琴
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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