A kind of sodium borohydride modified biochar and its preparation method and application
A sodium borohydride and biochar technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as affecting the extracellular electron transfer ability of iron-reducing bacteria, and achieve excellent extracellular electron transfer, environmental protection, etc. Friendly, low-cost effects
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Embodiment 1
[0033] A preparation method of sodium borohydride modified biochar, comprising the following steps:
[0034] (1) Place the dried rice straw in a tube furnace, and in a nitrogen protective atmosphere, raise the temperature of the biomass material to 600°C at a heating rate of 5°C / min for oxygen-limited high-temperature pyrolysis treatment for 1 hour, and cool to Take it out after room temperature, grind it, and pass through a 200-mesh sieve to prepare biochar.
[0035] According to the mass volume ratio of 1g: 100mL, mix the biochar prepared in step (1) with ultrapure water, stir evenly, let it stand for 24 hours, then wash it with ultrapure water until neutral, dry it for later use, and obtain the biochar before reaction. Carbon, denoted as WBC.
[0036] (2) The biochar prepared in step (1) was added into ultrapure water to obtain a biochar suspension, and the biochar concentration in the biochar suspension was 10 g / L.
[0037] (3) According to the mass ratio of biochar and ...
Embodiment 2
[0044] An application of sodium borohydride modified biochar in the reduction of ferric iron by iron-reducing bacteria, specifically using sodium borohydride modified biochar to promote the reduction of ferric iron by Geobacter, comprising the following steps:
[0045] In a 120mL serum bottle, add 0.5g of sodium borohydride modified biochar prepared in Example 1, 28mM ferric citrate, and 100mL Geobacter culture medium, and inoculate G .sulfurreducens were cultivated to complete the reduction treatment of ferric iron.
[0046] Blank group: the sodium borohydride modified biochar prepared in Example 1 was not added, and other conditions were the same as in Example 2.
[0047] Control group: the sodium borohydride modified biochar prepared in Example 1 was replaced by biochar, and other conditions were the same as in Example 2.
[0048] Determination of the concentration of ferrous iron, ferric iron and total iron in different systems, as well as the concentration of soluble fer...
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