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Lactobacillus paracasei for relieving diarrhea colon and application of lactobacillus paracasei

A technology of Lactobacillus and paracheese, applied in the field of microorganisms, can solve the problems of different effects of constipation, achieve the effect of shortening the time of black stool, increasing the level of GDNF, and increasing the water content of feces

Active Publication Date: 2021-06-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As an effective adjuvant treatment to relieve constipation, probiotics have a good laxative effect, but due to various causes of constipation, probiotics have different effects on different types of constipation
However, in the current study, probiotics have not been included in the treatment of laxative colon

Method used

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  • Lactobacillus paracasei for relieving diarrhea colon and application of lactobacillus paracasei
  • Lactobacillus paracasei for relieving diarrhea colon and application of lactobacillus paracasei
  • Lactobacillus paracasei for relieving diarrhea colon and application of lactobacillus paracasei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The screening, identification and cultivation of embodiment 1 Lactobacillus paracasei CCFM1164

[0042] (1) Isolation and screening of bacterial strains:

[0043] (1) Use a disposable sterile toilet to collect the feces of Liang Changgui, an 84-year-old man in Nan’an District, Chongqing, spread the feces samples in MRS medium, and place them in an anaerobic incubator (N 2 :CO 2 :H 2 =80:10:10) enriched for 12h;

[0044] (2) The enriched samples were serially diluted and spread on LB solid plates for anaerobic culture for 20 h;

[0045] (3) Select a single colony conforming to the basic form of Lactobacillus for plate streaking and purification, and screen and isolate the selected strain;

[0046] (4) The above-mentioned single colonies were cultured in liquid MRS culture medium for 20 hours, and then Gram staining was performed, and Gram-positive bacteria were selected for subsequent tests.

[0047] (2) Preliminary identification of Lactobacillus: calcilytic circle...

Embodiment 2

[0072] Embodiment 2: the alleviating effect of Lactobacillus paracasei CCFM1164 to colon mice of senna leaf laxative

[0073] Take Lactobacillus paracasei CCFM1164 out of the -80°C refrigerator, streak it on the MRS plate, culture at 37°C for 20 hours, pick a single colony in the MRS liquid tube, culture it at 37°C for 16 hours, and inoculate it at a volume of 2%. In the new MRS liquid medium, culture at 37°C for 16h, and culture for another generation in the same way, then centrifuge the suspension of Lactobacillus paracasei at 6000r / min, 4°C for 5min, and then wash with 10% skimmed milk Resuspended to obtain a cell concentration of 5×10 9 CFU / mL bacterial suspension for animal experiments.

[0074] Twenty-four healthy male C57BL / 6J mice aged 7 weeks were taken, fed for 1 week to adapt to the environment, and randomly divided into 4 groups: control group (normal), model group (model), mosapride group (mosapride) and side cheese group The Lactobacillus CCFM164 intervention g...

Embodiment 3

[0084] Embodiment 3: Lactobacillus paracasei CCFM1164 improves the level of mucin MUC-2 in the colon tissue of laxative colon mice

[0085] C57BL / 6J mice grouping, modeling and treatment methods were the same as in Example 2. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression level of MUC-2 gene. First, RNA was extracted from fresh colon tissue. The specific method was as follows:

[0086] Grind 0.2 g of fresh colonic tissue taken out of the mouse after dissection in a mortar with liquid nitrogen (180°C, 4h high temperature to inactivate the enzyme), then add 1mL Trizol reagent to the mortar, and continue grinding until the liquid is basically clear After that, collect it into a 1.5mL enzyme-free centrifuge tube, let it stand at room temperature for 15min, add 200μL of chloroform solution to the centrifuge tube, shake gently for 15s, let it stand at room temperature for 10min, centrifuge at 12000r / min at 4℃ for 15min, an...

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Abstract

The invention discloses lactobacillus paracasei for relieving diarrhea colon and application of the lactobacillus paracasei, and belongs to the technical field of microorganisms. The lactobacillus paracasei CCFM1164 provided by the invention can shorten the time of first black stool, improve the water content of stool, improve the transcriptional level of mucoprotein MUC-2 in colon tissues, reduce the transcriptional level of aquaporin AQP4 in the colon tissues, increase the number of Cajal interstitial cells and glial cells in the colon tissues, improve the glial cell-derived nutritional factor GDNF level and the butyric acid level in the colon tissues, improve the health state of the intestinal tract, and relieve the diarrhea of the colon fundamentally. The invention can be applied to functional foods and medicines for relieving diarrhea colon, and has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus paracasei for relieving laxative colon and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Laxative colon is a condition in which the long-term use of laxatives damages the colonic nervous system, leading to degenerative changes in enteric nerves, gastrointestinal motility disorders, and decreased responsiveness to laxatives, making patients dependent on laxatives. Middle-aged and elderly people suffering from constipation. [0003] In my country, the incidence of chronic constipation is as high as 20%. A series of stimulant laxatives such as senna, rhubarb, and aloe are favored by patients with constipation due to their quick effect and low cost. Patients need to use laxatives for a long time due to constipation. The dose of laxatives is inversely proportional to the effect, and finally loses the cathartic effect, forming a "laxative colon". In se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23C19/06A23C13/16A23L11/50A23L11/45A23L11/60A23L33/135A23L19/00A23L29/00A23G3/36A61K35/747A61P1/14A61P1/10C12R1/225
CPCA23C9/1234A23C19/062A23C13/16A23C11/106A23C20/025A23L33/135A23L19/00A23L29/065A23G3/366A61K35/747A61P1/14A61P1/10A23V2002/00A23V2400/165A23V2200/3204Y02A50/30
Inventor 王琳琳王刚李鑫萍赵建新陈卫张灏
Owner JIANGNAN UNIV
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