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Purification treatment method for soluble sugar in fruits and vegetables

A technology for purification treatment and soluble sugar, which is applied in the purification of sugar juice, chemical instruments and methods, and purification by physical methods, etc. It can solve the problems of reducing column efficiency, complicated operation, dead adsorption, etc. Guaranteed effect of purity

Inactive Publication Date: 2021-06-01
HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various derivatization methods have the characteristics of complex operation and reagent toxicity; cation columns, anion columns and sugar-specific columns all have the problem of poor separation performance
Currently, amino column and amide column have better column separation effect, but these two chromatographic columns have strong adsorption for acidic components such as organic acid and Vc with strong polarity, and are easy to form dead adsorption to reduce column efficiency; Low polysaccharides and proteins have good retention performance on these two chromatographic columns. They are not easy to be washed out, and they will also accumulate in the chromatographic column, reducing column efficiency.

Method used

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  • Purification treatment method for soluble sugar in fruits and vegetables
  • Purification treatment method for soluble sugar in fruits and vegetables
  • Purification treatment method for soluble sugar in fruits and vegetables

Examples

Experimental program
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preparation example Construction

[0043] Further, the flavone adsorption material is flavone glycoside adsorption resin APBA / pGMA / macroporous resin. In order to ensure the flavone adsorption effect, the preparation method of the macroporous resin comprises the following steps:

[0044] Pretreatment of macroporous resin;

[0045] Using glycidyl methacrylate as a functional monomer, graft organic long-chain polymers on the surface of the macroporous resin through free radical chain reaction to obtain the grafted material pGMA / macroporous resin;

[0046] Using 3-aminophenylboronic acid as the ring-opening reaction substance, the boronic acid group is introduced into the chain link of the organic long-chain polymer through the epoxy ring-opening reaction, and the flavone glycoside adsorption resin APBA / pGMA / macroporous resin is obtained.

Embodiment 1

[0049] In this embodiment 1, the method provided by the present invention comprises the following steps:

[0050] S101: Prepare a sample solution to obtain a soluble sugar stock solution containing impurities; specifically, take 1.0 g of fruit and vegetable pulp, add 10 mL of 80% ethanol solution, ultrasonically extract in a water bath at 40 °C for 30 min, and centrifuge at 4 °C and 10000 rpm for 10 min to obtain The supernatant and the residue were re-extracted once with 5 mL of 80% ethanol solution, the supernatants were combined, and the volume was adjusted to 25 mL with 80% acetonitrile to obtain the soluble sugar stock solution.

[0051] S102: extracting the soluble sugar stock solution, and purifying or enriching the soluble sugar stock solution, so as to separate soluble sugar and impurities in the soluble sugar stock solution, and obtain a filtrate to be tested. Specifically, take the soluble sugar stock solution, add organic acid adsorption material, polyphenol adsorpti...

Embodiment 2

[0059] In this embodiment 2, the method provided by the present invention comprises the following steps:

[0060] S201: Prepare a sample solution to obtain a soluble sugar stock solution containing impurities; specifically, take 1.0 g of fruit and vegetable pulp, add 10 mL of 80% ethanol solution, ultrasonically extract in a water bath at 40 °C for 30 min, and centrifuge at 4 °C and 10000 rpm for 10 min to obtain The supernatant and the residue were re-extracted once with 5 mL of 80% ethanol solution, the supernatants were combined, and the volume was adjusted to 25 mL with 80% acetonitrile to obtain the soluble sugar stock solution.

[0061] S202: extracting the soluble sugar stock solution, and purifying or enriching the soluble sugar stock solution, so as to separate soluble sugar and impurities in the soluble sugar stock solution, and obtain a filtrate to be tested. Specifically, take the soluble sugar stock solution, add organic acid adsorption material, polyphenol adsorp...

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Abstract

The invention relates to a purification treatment method for soluble sugar in fruits and vegetables, wherein the method comprises the steps: preparing a sample solution to obtain a soluble sugar stock solution containing impurities, extracting the soluble sugar stock solution, and purifying or enriching the soluble sugar stock solution to separate soluble sugar and impurities in the soluble sugar stock solution to obtain a filtrate to be detected. By adding one or more solid powder adsorption materials which have adsorption effects on organic acids, polyphenols, flavones, chlorophyll and the like and do not adsorb monosaccharides, impurity removal of fructose determination samples is realized, the purity of soluble sugar determination samples is ensured, and the subsequent determination accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of fructose purification, in particular to a method for purifying soluble sugar in fruits and vegetables. Background technique [0002] The chemical composition of fruits and vegetables is very complex: including chlorophyll, fatty acids, terpenes, flavonoids, nucleosides, polyphenols, organic acids, water-soluble vitamins, amino acids, monosaccharides, disaccharides, polysaccharides, proteins, etc. Many components will Cause interference to different types of liquid phase analysis, or form dead adsorption on the chromatographic column, shorten the service life of the chromatographic column, increase detection instability and cost. [0003] At present, the determination of monosaccharides and disaccharides mainly includes pre-column derivatization gas phase (gas) chromatography, pre-column derivation liquid chromatography, ion chromatography, and liquid chromatography. All kinds of derivatization methods ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13B20/12C13B20/16C13B20/14B01J20/24B01J20/26B01J20/28B01J20/30
CPCC13B20/12C13B20/16C13B20/165C13B20/146C13B20/123B01J20/24B01J20/264B01J20/28085
Inventor 赵江丽关军锋程玉豆
Owner HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY