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Porous magnetic diagnosis and treatment agent, preparation method and use

A technology of photosensitizer and ferric tetroxide, which is applied in the field of biomedicine to achieve the effect of high-efficiency loading

Active Publication Date: 2021-06-18
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Researchers have designed fluorescent magnetic nanoprobes that can combine photosensitizer-mediated fluorescence imaging with magnetic resonance imaging, but these nanoprobes are harmful to normal organs (liver, kidney, spleen, lung, etc.) Injury and accumulation of tumor tissue sites did not achieve the desired effect

Method used

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  • Porous magnetic diagnosis and treatment agent, preparation method and use
  • Porous magnetic diagnosis and treatment agent, preparation method and use
  • Porous magnetic diagnosis and treatment agent, preparation method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0060] (1) Preparation of ferric oxide nanoparticles: The division weighs 300 mmoL of ferrous sulfate and 300 mmoL of ferric citrate and dissolves it in 100 mL of deionized water to prepare a solution, and stirs at 400 rpm to mix the solution completely; then add 500 mmoL of ascorbic acid, And add 0.3M sodium hydroxide to adjust the pH value of the solution to 9, after mixing and stirring again at 400rpm for 30min; transfer the solution to a hydrothermal reactor, react at 200 ° C for 5h, use a dialysis bag for dialysis, set Store in a refrigerator at 4° C. to obtain a dispersion suspension of ferric oxide nanoparticles. The dispersion suspension of the ferric oxide nanoparticles is adapted to a centrifuge for high-speed centrifugation, and the centrifuged product is dried to obtain the ferric oxide nanoparticles.

[0061] (2) Loading photosensitizer drug: Take 10 mL of the dispersion suspension of ferric oxide nanoparticles prepared above and uniformly mix with 10 mL of photos...

Embodiment 2

[0066] For in vitro tumor synergistic treatment, a mixture of physiological saline and diagnostic and therapeutic agent (the solvent is physiological saline, and the concentration of the diagnostic and therapeutic agent is 5 mg / mL) is placed in two 24-well cell culture plates, and sterilized for 2 hours. The PC3 cells were plated at 5 x 10 4 Cells / well were seeded into 24-well cell culture plates and cultured overnight. Then, cells in the first and second plates were irradiated with an 808 nm (NIR I biowindow) laser for 5 minutes, respectively. CCK-8 studies the metabolic activity of PC3 cells before and after laser irradiation. The absorbance of CCK-8 after 1 hour incubation with cells was read at 450 nm using a microplate reader (MK3, Thermo, USA).

[0067] test results image 3 As shown, there is no significant difference in the cell viability in the mixture of physiological saline and the diagnosis and treatment agent without laser excitation, indicating that the diagno...

Embodiment 3

[0070] will be about 10 7 PC3 cells were injected subcutaneously into the back of nude mice and fed for a period of time until the tumor volume reached 200 m 3 . The nude mice were divided into two groups, one group was injected with physiological saline, and the other group was injected with a diagnosis and treatment agent (the solvent was physiological saline, the concentration of the diagnosis and treatment agent was 5 mg / mL), and the physiological saline and the diagnosis and treatment agent were injected every 5 days, respectively. Then use 808nm (NIR Ibiowindow) laser to irradiate the tumor site, use FLIR TM The E60 camera records thermal images and temperatures of laser-irradiated mice. Nude mice were fed for 25 days to form tumor lumps, then, the tumor lumps were excised, and the tumor treatment effect was evaluated by measuring the volume and appearance of the tumor.

[0071] result Figure 4 and Figure 5 As shown, after injecting the diagnostic agent, the magn...

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Abstract

The invention relates to the field of biomedicines and particularly relates to a porous magnetic diagnosis and treatment agent, a preparation method and use. A method for preparing ferroferric oxide nanoparticles is characterized by at least comprising the steps of subjecting a mixed solution with a pH of 9-11, which contains inorganic iron, organic iron and ascorbic acid, to a hydrothermal reaction, and dialyzing a reaction product, so as to obtain a disperse suspension containing the ferroferric oxide particles. The ferroferric oxide nanoparticles are prepared by the method and have porosity and magnetic property. The diagnosis and treatment agent comprises the ferroferric oxide nanoparticles, a photosensitizer and an anticancer drug, wherein a mass ratio of the photosensitizer to the ferroferric oxide nanoparticles is (7.8-9.8): 100, and a mass ratio of the anticancer drug to the ferroferric oxide nanoparticles is (4-6): 100. The diagnosis and treatment agent prepared by the method can efficiently load drugs and has a magnetic targeting effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a porous magnetic diagnosis and treatment agent, a preparation method and an application. Background technique [0002] Prostate cancer is one of the most common male malignancies in the world. Its onset is relatively insidious. Many patients are already in the advanced stage of the disease when they are first diagnosed, with poor survival prognosis. Androgen therapy can achieve a good therapeutic effect on prostate cancer in the early stage of the disease, but once the disease progresses to castration resistant prostate cancer (CRPC), it is difficult to achieve androgen therapy. CRPC is not sensitive to conventional radiotherapy and chemotherapy, and a large number of studies have confirmed that it is related to the progression of prostate cancer, tumor metastasis, and resistance to radiotherapy and chemotherapy. At present, individualized comprehensive treatment for CRPC is currentl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K45/06A61P35/00A61K49/00A61K49/18A61K31/337
CPCA61K41/0057A61K45/06A61K31/337A61P35/00A61K49/0002A61K49/0019A61K49/0093A61K49/1818Y02A50/30
Inventor 谭海颂侯楠刘岩磊徐斌王忠
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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