A kind of dendrobium officinale oligosaccharide, dendrobium officinale oligosaccharide derivative and its preparation method and application

A technology of Dendrobium officinale and derivatives, applied in the field of natural medicinal chemistry, to achieve good immune regulation and strong anti-aging effects

Active Publication Date: 2022-02-25
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These predecessors' research work mainly focused on the pharmacological activity of small molecule phenolic components and polysaccharide components, and there were few reports on the research on oligosaccharides.

Method used

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  • A kind of dendrobium officinale oligosaccharide, dendrobium officinale oligosaccharide derivative and its preparation method and application
  • A kind of dendrobium officinale oligosaccharide, dendrobium officinale oligosaccharide derivative and its preparation method and application
  • A kind of dendrobium officinale oligosaccharide, dendrobium officinale oligosaccharide derivative and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0089] Method for preparing iron dendrobium oligosaccharide derivative, including the following steps:

[0090] 1) Reduce fatty acid into fatty alcohol;

[0091] Preferably, the reaction process is: fatty acid 10 mmol, NABH 4 20mmol, Alcl 3 40 mmol, THF 27 mL, MeOH3ml, the reaction temperature was 100 ° C, the reaction time was 8 h, and the yield was 90%;

[0092] 2) Glycoside in fatty alcohols with iron dendrobium;

[0093] Preferably, the fatty alcohol obtained by the reduction is carried out with a glycoside reaction in a tape dendrobium wholly sugar, and the reaction process is: the alcohol gum ratio 1: (8 ~ 10), catalyst hexagonyl bromide, the reaction temperature is 110 ~ 115 ° C, the reaction time was 3 h, and the yield was 80%.

Embodiment 1

[0096] This embodiment is mainly to purify the iron leather oligosaccharide from the stem of Tieji Dendrobium, and further structural identification:

[0097] The fresh stems were cut off in the blast dryer at 55 ° C for drying in the blast dryer, and the water was measured by 6% or less, and the water was pulverized over 20-40 mesh sieve; Take the iron dendrobium powder 50 grams of ultrapure water 2500ml, Loose water bath 2 times, 2 hours each time, with water purification. After the centrifugation, centrifugation conditions: 3000-4000 rpm, 10 min, 25 ° C room temperature, obtained aqueous Dendrobium aqueous extracts; concentrated under reduced pressure to 500 mL to obtain a concentrated water extract of Iron Dendrobium. Add the amount of 95% ethanol to the mixed water liquid solution to the iron dendrobium to the mixed water liquid alcohol concentration of 70-80%, overnight precipitation, after centrifugation, centrifugal conditions: 4000 rpm, 20min, room temperature 25 ° C, to ...

Embodiment 2

[0100] DPPH free radical clearance experiment

[0101] 1 reagent:

[0102] DPPH, water-soluble vitamin E (Trolox) (Trolox is dissolved in anhydrous ethanol, concentration of 10 mm, -20 ° C), pure water.

[0103] 2 experimental method:

[0104] The drug to be tested with DPPH is mixed with DPPH (a final concentration of 100 m), and three repeating holes are set, and the white-controlled hole and Trolox positive control holes, 30 ° C, 1H, and the enzyme gauge measuring OD value, detection The wavelength was 515 nm, and the antioxidant rate was calculated.

[0105] Antioxidant rate (%) = (1-experimental hole OD 515nm / Blank hole OD 515nm ) × 100%

[0106] 3 Experimental results:

[0107] As shown in Table 1.

[0108] Table 1

[0109] sample Final reaction concentration Antioxidant rate (%) Trolox 25 95.668％ Iron dendrobide 4％ 11.335％ Iron dendrobide 8％ 14.346％ Iron dendrobide 11％ 16.213％ Iron dendrobide 15％ 21.155％ Iron dendrobide 20％ 2...

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Abstract

The invention relates to a Dendrobium officinale oligosaccharide, a Dendrobium officinale oligosaccharide derivative and a preparation method and application thereof. The main technology is that the Dendrobium officinale oligosaccharide contains 3-9 glycoside residues and has glucose at the non-reducing end residue; its derivatives are obtained by substituting hydrophobic residues at the non-reducing end, and the preparation method and application of the two are also provided. The beneficial effects of the present invention are as follows: for the first time, Dendrobium candidum oligosaccharides with a purity ≥ 99% were prepared from Dendrobium candidum, and the structure, anti-aging activity and immune regulation of Dendrobium candidum oligosaccharides were clarified, and it was found that it can well inhibit Collagenase also has certain activity on TNF-α and IL-6; it can be used in medicine, food, daily necessities development and other fields.

Description

Technical field [0001] The present invention relates to the field of natural pharmaceutical chemistry, and in particular, there is a method and application of tin dendrobide oligosaccharides, iron dendrobium oligosaccharide derivatives. Background technique [0002] Dendrobium Officinale Kimura et migo is the perennial plants in Orchidaceae, which is contained in various version of the pharmacology, and the pharmacopoeia records it has a stomach to nourish the stomach; nourishing yin and heat; moisturizing lungs and kidney; graphic strong waist. Modern Pharmacology Studies have shown that Iron Dendrobium has antioxidant, hypoglycemic, regulatory immunity and anti-inflammatory activity. In ancient books, Iron Dendrobium can be used for skin moisturizing and thirsty. The study found that the dendrobium derivedlium contained polysaccharides, 菧, flavonoids, phtophysical, lignin and other chemical components. [0003] Aging is the inevitable result of human growth and how to delay the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/08A61Q19/00A61K31/715A61P17/18A61P39/06A61P37/02A23L33/125
CPCC08B37/0003A61K8/73A61Q19/08A61Q19/00A61K31/715A61P17/18A61P39/06A61P37/02A23L33/125A61K2800/782A23V2002/00A23V2200/324A23V2200/318A23V2250/51A61K2800/522A23L33/105C08B37/0006C08B37/0033C08B37/0087A61K31/702A61K9/0014Y02A50/30A61K9/06A61K31/716A61K36/8984A61K2236/331A61K2236/333A61K2236/51A61K2236/53B01D11/0288B01D21/262C07H3/06C08B37/006
Inventor 胡江苗杨柳解勇王雨华贺锐
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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