Targeted drug co-delivery nano system, and preparation method and application thereof

A technology of co-delivery and drugs, applied in drug delivery, nanotechnology, nano-drugs, etc., can solve the problems of ineffective inhibition of tumor growth and anti-apoptosis, weakening of chemotherapy drugs, insufficient expression, etc., to enhance drug efficacy and improve Circulation time, effect of promoting apoptosis

Active Publication Date: 2021-06-25
PEOPLES HOSPITAL OF HENAN PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, miRs are underexpressed in ovarian cancer cells, cannot effectively inhibit tumor growth and anti-apoptosis, and weaken the killing effect of chemotherapy drugs on tumor cells.

Method used

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  • Targeted drug co-delivery nano system, and preparation method and application thereof
  • Targeted drug co-delivery nano system, and preparation method and application thereof
  • Targeted drug co-delivery nano system, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A targeted drug co-delivery nanosystem, including gold nanorods, the surface of the gold nanorods is coated with amino-modified mesoporous silica, and the amino-modified mesoporous silica and gold nanorods form a co-carrier to load targeted drugs. And the amino-modified mesoporous silica is sequentially modified by PEG and hyaluronic acid.

[0065] The preparation process of targeted drug co-delivery nanosystem includes the following steps:

[0066] 1) Take 5mL of 0.1M cetyltrimethylammonium bromide (CTAB), add 125μL of 1% HAuCl to it 4 , after stirring well, add 300 μL of 0.01M NaBH 4 , after the color of the solution changed from golden yellow to brown, place it at 30°C for 2h to obtain a seed solution; mix 7mL of 0.1M CTAB and 300μL of 1% HaCl 4 The solution was mixed, and 10 μL of 0.1 M AgNO was added to the mixture 3 . Then, add 120 μL of 0.33M hydroquinone to the mixture, and the solution quickly becomes colorless, and the growth solution is obtained; add 100 ...

experiment example 1

[0080] The product obtained in each step of Example 1 was systematically studied on its morphology, composition, chemical bond and microstructure by XRD, HAADF-STEM, HRTEM, FTIR, zeta, BET and other modern nano-testing and analysis techniques, and the results are as follows:

[0081] The present invention adopts X-ray diffraction (XRD; X'Pert-PRO-MPD, Hol-land-Panalytical) with monochromatic X-ray beam and nickel to filter Cu-Ka radiation to analyze the crystal structure of GNRs obtained by the embodiment, and the results are as follows figure 1 As shown, the XRD pattern clearly shows that the diffraction of gold nanorods occurs at 2θ of 38.2°, 44.4°, 64.6°, 77.6° and 81.7°, corresponding to (111), (200), (220), ( 311), (222) crystal faces, indicating that the present invention successfully synthesizes gold nanorods.

[0082] The present invention adopts the high-resolution transmission electron microscope (HRTEM) of high-angle annular dark field scanning transmission electron...

experiment example 2

[0088] Biosafety and fluorescence performance evaluation of HA-pGNR@MSN co-carrier nanosystem

[0089] 2.1 In vitro cytotoxicity test

[0090] Cell culture: The ovarian cancer SKOV3 cell line used in the present invention was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). SKOV3 cells were cultured in RPMI 1640 medium containing 10% FBS, 100 μg / mL penicillin and 100 μg / mL streptomycin at 37 °C, 5% CO 2 Humidify the air. SKOV3 TR The cell line (PTX-resistant SKOV3 cells) was maintained in 10 μL of PTX solution with a concentration of 2 mg / mL in DMSO in RPMI 1640 medium.

[0091] Low cytotoxicity is crucial for constructing targeted drug co-delivery nanosystems. The present invention detects HA-pGNR@MSN (HA-pGNR@MSN and The difference of Example 1 of the present invention is that the preparation process omits the cytotoxicity of PTX and miR-let-7, and the others are the same). The specific process is as follows: SKOV3 cells are s...

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Abstract

The invention discloses a targeted drug co-delivery nano system. The nano system is characterized in that a nano co-carrier with relatively strong drug loading capacity is formed by coating gold nanorods with mesoporous silica, and targeted co-delivery of a chemotherapeutic drug PTX and a gene drug miR let-7alpha is realized by combining modification of amino, PEG and HA. The invention also discloses a preparation method of the targeted drug co-delivery nano system. The method is simple to operate, and the size of the prepared product is controllable. The invention also discloses application of the targeted drug co-delivery nano system in preparation of drugs for treating ovarian cancer. The co-delivery nano system realizes effective delivery of chemotherapeutic drugs and gene drugs, can target tumor tissues, increases permeability of tumor parts, promotes drug uptake of cancer cells, and is beneficial to improvement of drug effect, reversal of multidrug resistance of ovarian cancer tissues and enhancement of PTX treatment effect, thereby improving treatment efficiency. A new thought is provided for treating the ovarian cancer.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, and relates to a targeted drug co-delivery nano system and its preparation method and application. Background technique [0002] Ovarian cancer is one of the most serious cancers that endanger women's lives and health, and its morbidity and mortality rank first among gynecological malignancies. At present, the main strategy for clinical treatment of ovarian cancer is based on chemotherapy drugs such as paclitaxel (PTX) and platinum, combined with surgery. However, during chemotherapy, cancer cells often develop multidrug resistance (MDR) to chemotherapeutic drugs, which leads to the insensitivity of patients to therapeutic drugs, poor chemotherapy effect, and eventually leads to chemotherapy failure and even disease recurrence. According to statistics, 50%-75% of ovarian cancer patients eventually relapse due to MDR. The high expression of multidrug resistance-related genes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/60A61K47/61A61K31/337A61K31/7088A61K47/02A61K47/04A61K47/69A61P15/08A61P35/00B82Y5/00
CPCA61K47/61A61K47/60A61K47/6923A61K47/02A61K31/337A61K31/7088A61P35/00A61P15/08B82Y5/00A61K2300/00Y02A50/30
Inventor 郭玉琪王雪琴张川靳林贺红英熊田崔苗任志帅马沈倩
Owner PEOPLES HOSPITAL OF HENAN PROV
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