Immobilized enzyme catalyst, preparation method thereof and application of immobilized enzyme catalyst in synthesis of vitamin A palmitate

A technology for immobilizing enzymes and catalysts, which can be used in biochemical equipment and methods, enzymes, etc., which are immobilized on or in inorganic carriers, and can solve the problems of acid resistance, poor hydrophobicity and diffusivity, and achieve high physical and chemical stability. Use efficiency, enhance the effect of structural stability

Active Publication Date: 2021-07-02
WANHUA CHEM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of immobilized enzyme catalyst and its preparation method and its application in synthetic vitamin A palmitate, this immobilized enzyme utilizes electrospinning technology to synthesize the nanorod of polymer modified metal oxide, Effectively solve the problems of poor acid resistance, hydrophobicity and poor diffusion of traditional enzyme catalysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of immobilized enzyme catalyst:

[0048] Dissolve 5.0 g of free enzyme (Candida sp.99-125 dry enzyme powder) in 250 mL of 0.2 mol / L sodium hydrogen phosphate buffer, adjust the pH to 4.5, and store it under nitrogen atmosphere with stirring.

[0049] 10.0 g of tetrabutyl titanate was weighed and added to 100 mL of ethanol / acetic acid mixed solution (the volume ratio of ethanol to acetic acid was 0.5:1). Weigh 1g of α-cellulose and add it into 50mL of methanol, heat up to 30°C and stir vigorously to disperse evenly. Add the mixed solution of tetrabutyl titanate above to the cellulose methanol solution dropwise, and after adding dropwise for 1 hour, transfer the resulting mixture to an electrospinning syringe, and after electrospinning at 10kV for 5 hours, place the obtained solid in a nitrogen atmosphere , baked at 300°C for 4h.

[0050] Accurately weigh 10.0 g of the roasted solid and add it to the above-mentioned sodium hydrogen phosphate buffer solution ...

Embodiment 2

[0056] Preparation of immobilized enzyme catalyst:

[0057] Dissolve 5.0 g of free enzyme (Candida sp.99-125 dry enzyme powder) in 300 mL of 0.3 mol / L sodium hydrogen phosphate buffer, adjust the pH to 5.0, and store it under nitrogen atmosphere with stirring for later use.

[0058] Weigh 10.0 g of cobalt nitrate and add to 200 mL of ethanol / acetic acid mixed solution (the volume ratio of ethanol to acetic acid is 0.6:1). Weigh 2.0g of α-cellulose and add it into 400mL of methanol, heat up to 40°C and stir vigorously to disperse evenly. The above mixed solution of cobalt nitrate was added dropwise to the cellulose methanol solution, and after 1.2 h, the resulting mixture was transferred to an electrospinning syringe, and after electrospinning at 15 kV for 6 h, the resulting solid was placed in a nitrogen atmosphere, Calcined at 350°C for 4.5h.

[0059] Accurately weigh 10.0 g of the roasted solid and add it to the above-mentioned sodium hydrogen phosphate buffer solution con...

Embodiment 3

[0065] Modification and immobilized preparation of free enzyme:

[0066] Dissolve 5.0 g of free enzyme Candida sp.99-125 dry enzyme powder in 300 mL of 0.5 mol / L sodium hydrogen phosphate buffer, adjust the pH to 5.5, and store it under nitrogen atmosphere with stirring. 10.0 g of tetrabutyl titanate was weighed and added to 300 mL of ethanol / acetic acid mixed solution (the volume ratio of ethanol to acetic acid was 0.8:1). Weigh 3.0g of pectin and add it to 600mL of methanol, heat up to 60°C and stir vigorously to disperse evenly.

[0067] Add the mixed solution of tetrabutyl titanate above to the pectin methanol solution dropwise, and after 1.8h, transfer the resulting mixture to the syringe for electrospinning, and after electrospinning at 18kV for 8h, put the obtained solid under nitrogen In the atmosphere, bake at 450°C for 6.5h. Accurately weigh 10.0 g of the roasted solid and add it to the above-mentioned sodium hydrogen phosphate buffer solution containing free enzym...

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Abstract

The invention discloses an immobilized enzyme catalyst, a preparation method thereof and application of the immobilized enzyme catalyst in synthesis of vitamin A palmitate. According to the method, a metal oxide nanorod is synthesized by using an electrostatic spinning technology, the surface of the nanorod is modified by using a hydrophobic natural organic polymer, lipase is immobilized on the surface of a modified carrier by using a sound wave-assisted method, and the problems of poor acid resistance, hydrophobicity and diffusivity of traditional enzyme catalysis are effectively solved. The invention also provides the application of the immobilized enzyme in synthesis of vitamin A palmitate, the process does not need an organic solvent, is green and environment-friendly, and the immobilized enzyme can be used mechanically in a long batch and has extremely high physical and chemical stability.

Description

technical field [0001] The invention belongs to the technical field of chemical synthesis, and in particular relates to an immobilized enzyme catalyst and a preparation method thereof and its application in synthesizing vitamin A palmitate. Background technique [0002] Compared with vitamin A acetate (VA acetate), vitamin A palmitate (VA palmitate) has the advantages of long carbon chain, low melting point, good oil solubility, and good low temperature stability. An indispensable substance that has been widely used in cosmetics, medicines, feed, etc. [0003] Esterification or transesterification in the presence of lipase to obtain VA palmitate is one of the common methods for preparing VA palmitate. Patent JP62248495 discloses a lipase-catalyzed method for synthesizing VA palmitate, which reacts for 12 hours at a temperature of 30° C., and the yield is only 80%. After the reaction, it is difficult to separate the lipase. Patent CN101200740 discloses a method for synthesi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12N11/14C12P23/00
CPCC12N9/20C12N11/14C12P23/00C12Y301/01003
Inventor 罗朝辉林龙王嘉辉吕英东
Owner WANHUA CHEM GRP CO LTD
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