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Nucleic acid hydrogel with improved mechanical properties as well as preparation method and application thereof

A hydrogel and nucleic acid technology, applied in the field of polymer materials and biology, can solve the problems of changing the hydrogel network cross-linking structure and cross-linking density, topology, mesh distribution and permeability properties are no longer retained, etc. The effect of improving mechanical strength and stability and good biocompatibility

Active Publication Date: 2021-07-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the introduction of nanomaterials may bring unknown biological toxicity
In addition, these methods will change the crosslinking structure and crosslinking density of the hydrogel network while enhancing the mechanical properties, and the original topology, mesh distribution and permeability of the supramolecular hydrogel network will no longer be preserved.

Method used

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  • Nucleic acid hydrogel with improved mechanical properties as well as preparation method and application thereof
  • Nucleic acid hydrogel with improved mechanical properties as well as preparation method and application thereof
  • Nucleic acid hydrogel with improved mechanical properties as well as preparation method and application thereof

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preparation example Construction

[0116] The second aspect of the present disclosure provides the preparation method of the nucleic acid hydrogel according to the first aspect of the present disclosure, the step of cross-linking the scaffold unit with the cross-linking unit I and the cross-linking unit II.

[0117] In some embodiments, the preparation method of nucleic acid hydrogel comprises the following steps:

[0118] preparing scaffold units in the gel matrix to obtain a scaffold unit solution;

[0119] preparing crosslinking unit I and crosslinking unit II in the gel matrix to obtain a crosslinking unit solution;

[0120] The scaffold unit solution is mixed with the crosslink unit solution, and the scaffold unit self-assembles with crosslink unit I and crosslink unit II to obtain a nucleic acid hydrogel.

[0121]

[0122] In some embodiments, the scaffold core in the scaffold unit is nucleic acid, and the scaffold unit can be prepared by complementary pairing of nucleic acid fragments. Exemplarily, t...

Embodiment 1

[0176] Example 1 Preparation of nucleic acid hydrogels

[0177] Step 1, use the rolling circle amplification reaction to prepare long strands of single-stranded DNA, as follows:

[0178] Mix 350 pmol of linear template strand, 10 μL of 10× ssDNA / RNA circular ligase reaction buffer, 3 μL of ssDNA / RNA circular ligase, 5 μL of 50 mM MnCl 2 Mix well, and add an appropriate amount of high-purity water to a total volume of 100 μL. Incubate at 60°C for 6h for circularization reaction, then react at 80°C for 10min to inactivate the ligase, and place at room temperature for 10min to cool down to room temperature to obtain circular template DNA.

[0179] Add 5 μL exonuclease I and 2.5 μL exonuclease III to the above system. React at 37°C for 30min to digest the uncyclized linear template strand, then inactivate at 90°C for 5min, and cool at room temperature for 10min. After the reaction product was subjected to 20% acrylamide / 8M urea denaturing gel electrophoresis, the situation ...

Embodiment 2

[0187] Example 2 Preparation of nucleic acid hydrogels

[0188] The nucleic acid hydrogel was prepared by the method shown in Step 1-Step 4 in Example 1, wherein the preparation of the cross-linking unit solution in Step 3 was as follows:

[0189] Take 28.50nmol of L1 and L2 each, mix the single-stranded DNA solution containing 3.0nmol of the long chain of the repeat sequence and 3.0nmol of the complementary strand and freeze-dry to obtain a cross-link with a molar content of repeat fragments in the cross-link unit II of 5.0%. unit solution.

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Abstract

The invention belongs to the technical field of high polymer materials and biology, and particularly relates to nucleic acid hydrogel with improved mechanical properties and a preparation method and application thereof. The nucleic acid hydrogel is formed by respectively cross-linking a scaffold unit with a cross-linking unit I and a cross-linking unit II, and the nucleic acid hydrogel has improved mechanical strength and stability. Meanwhile, the nucleic acid hydrogel keeps the dynamic characteristics of supramolecular hydrogel, has the properties of rapid prototyping, shear thinning, injectability, self-repairing property, thermoplasticity and the like, also has good biological compatibility, has good application prospects in the biomedical fields of drug delivery, cell culture differentiation, protein production, immuno-regulation and the like, and has important application prospects in the flexible electronic fields of wearable equipment, artificial skin, soft robots and the like.

Description

technical field [0001] The disclosure belongs to the field of polymer materials and biotechnology, and in particular, the disclosure relates to a nucleic acid hydrogel and its preparation method and application. Background technique [0002] Supramolecular hydrogel is a kind of soft matter material with three-dimensional network structure formed by self-assembly based on non-covalent interactions. Compared with covalently crosslinked hydrogels, the transient reversibility of noncovalent interactions endows supramolecular hydrogels with favorable dynamic properties, such as stimuli responsiveness, injectability, shape adaptability and Self-healing properties, etc., have shown great application potential in many fields such as biomedicine, flexible electronic materials, and soft robots. However, the fast dissociation and recombination rates of supramolecular interactions lead to poor mechanical properties of supramolecular hydrogels, which limits the application of supramolec...

Claims

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Application Information

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IPC IPC(8): B01J13/00
CPCB01J13/0052B01J13/0065
Inventor 刘冬生李宇杰杨勃
Owner TSINGHUA UNIV
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