Hydrogel microspheres for repairing bone defects and preparation method thereof

A technology for hydrogel microspheres and bone defects, applied in the field of biomedicine, can solve the problems of poor biocompatibility and degradability, rough and porous surface, complicated preparation process, etc. Simple, cell proliferation-promoting effect

Active Publication Date: 2021-07-30
上海泽充生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the shortcomings of existing bone repair materials such as complex preparation process, uneven particle size, poor biocompatibility and degradability, and high price, and provides a hydrogel microsphere for repairing bone defects and its preparation Method, the present invention uses microfluidic technology to prepare a kind of hydrogel microspheres for repairing bone defects with methacrylylated gelatin (GelMA) and nano-hydroxyapatite (nHAP) as raw materials, and its diameter is distributed in 300 Between ~500μm, the particle size is controllable, the physical and chemical properties are stable, the surface is rough and porous, and the biocompatibility is good, which can promote the proliferation, adhesion and differentiation of MC3T3-E1 cells

Method used

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  • Hydrogel microspheres for repairing bone defects and preparation method thereof
  • Hydrogel microspheres for repairing bone defects and preparation method thereof
  • Hydrogel microspheres for repairing bone defects and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] The preparation of embodiment 1 GelMA

[0052] Accurately weigh 10g type A pigskin gelatin, and measure 100mL PBS into a round bottom flask, heat and stir in a water bath at 50°C for 30min to dissolve, after the gelatin is dissolved, slowly add 10mL under the condition of dark and vigorous stirring MA. After the MA dropwise addition was complete, the reaction was allowed to proceed for 24 h with full stirring and protected from light. Afterwards, the product was transferred to a dialysis bag (3500 Da), dialyzed at 40°C, and the distilled water was changed every 4 hours for a total of 6 dialyses. Finally, the product was lyophilized to obtain a loose and porous white solid product, namely GelMA, which was stored at -20°C.

Embodiment 2

[0054] GelMA product characterization:

[0055] Take a small amount of freeze-dried product GelMA and ground gelatin respectively, and use a Fourier transform infrared spectrometer to scan its infrared spectrum in the range of 400-4000 wavelength. In addition, the use of gelatin before and after methacrylation 1 Characterized by H NMR, 20mg GelMA and gelatin were dissolved in 0.5mL D 2 O, and then loaded into a nuclear magnetic resonance tube and tested with a nuclear magnetic resonance spectrometer. gelatin 1 H NMR spectra are extremely complex because gelatin consists of >20 different amino acids. The structure of these amino acid building blocks is -NH-CHR-CO-, in which the side chain (R) changes and has different functional groups, and it is very difficult to complete the complete analysis of the spectrum. For the calculation of DS, a peak corresponding to a group that cannot be modified must be selected, by recording the peaks of different amino acids 1 H NMR spectro...

Embodiment 3

[0058] The preparation of embodiment 3 hydrogel microspheres

[0059] (1) Utilize the GelMA gained in Example 1 to disperse in water, prepare the GelMA solution of 10wt%, and add the mixed solution obtained after adding 0.25wt% LAP (photoinitiator) relative to the water quality, as the dispersed phase; Sesame oil as the continuous phase;

[0060] (2) The dispersed phase and the continuous phase in step (1) are injected into the microchannel respectively, and the dispersed phase forms monodisperse droplets due to the shear force under the promotion of two syringe pumps; wherein, the dispersed phase controls 4mL / h flow rate injection, continuous phase control 5mL / h flow rate injection. By adjusting the flow rates of the aqueous and oil phases, droplets with different size distributions can be obtained. Collected W / O emulsion at 25mW cm -2 Polymerized into microspheres after exposure to UV light (405 nm) for 60 s. Determine the size of the resulting microspheres using micros...

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Abstract

The invention discloses preparation of hydrogel microspheres for repairing bone defects and an application thereof, and belongs to the technical field of biological medicines. According to the preparation method, the hydrogel microspheres with different diameters and different proportions can be prepared by using a microfluidic device and taking methylacryloylated gelatin and nano-hydroxyapatite as raw materials through an ultraviolet crosslinking principle, and the hydrogel microspheres prepared by the preparation method have good mechanical stability, biocompatibility and osteogenesis regeneration capacity; the preparation method of the microspheres is simple, can be used for batch production, and has a good application prospect.

Description

technical field [0001] The invention relates to a hydrogel microsphere for repairing bone defect and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Bone damage can result from severe impact, tumor removal, or bone loss. About 800,000 surgical procedures are performed each year resulting in skeletal injuries, and this number is increasing rapidly as the population ages. Natural bone grafts do not meet clinical needs due to limited availability, postoperative infection and bleeding of autografts, and prevalent risk of spread and immune rejection of allografts and xenografts. Therefore, the number of synthetic bone grafts is increasing, which can achieve the therapeutic effects of autologous bone grafts without causing serious side effects. But many promising synthetic bone grafts, especially injectable bone grafts, consistently suffer from poor organic and inorganic compatibility. On the one hand, the mechanical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/44A61L27/58C08J3/075C08L89/00
CPCA61L27/46A61L27/446A61L27/58C08J3/075C08J2389/00A61L2430/02A61L2400/12C08L89/00
Inventor 周娟陈敬华李渊丽陈皓郭良雨邢健
Owner 上海泽充生物技术有限公司
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