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Soft tissue repair biological material and preparation method thereof

A biomaterial and soft tissue technology, applied in the field of medical engineering, can solve the problems of lack and limited clinical application effect, and achieve the effect of promoting cell adhesion

Inactive Publication Date: 2021-08-24
JIANGYIN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of active induction of tissue repair activities, such as the function of cell chemotaxis and activity, the function of promoting angiogenesis and the regulation of inflammatory response, synthetic scaffold materials have limited clinical application effect, especially for severe soft tissue defects. For repair, it is necessary to combine exogenous growth factors, negative pressure sealing drainage drugs or clinical techniques to achieve the purpose of tissue repair

Method used

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  • Soft tissue repair biological material and preparation method thereof
  • Soft tissue repair biological material and preparation method thereof
  • Soft tissue repair biological material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of soft tissue repair biomaterial, its preparation method comprises the steps:

[0035] (1) Dissolving type I collagen with 0.5M acetic acid solution to obtain 1w / v% type I collagen acetic acid solution;

[0036] (2) Take a 10cm proximal jejunum from a healthy adult pig, remove excess connective tissue and residual impurities in the intestine, then place the tissue in a refrigerator at 4°C for 10 hours, and then soak it in 1.0N sodium chloride saline for 6 hours to remove intestinal epithelial cells ; After that, the serosa and smooth muscle tissue were removed by mechanical scraping and hydration to obtain small intestinal submucosa tissue;

[0037] (3) Place the small intestinal submucosa tissue prepared in step (2) in a mixed solution of peracetic acid and ethanol (the volume fraction of oxyacetic acid is 0.1%, and the volume fraction of ethanol is 4%) to soak for 2 hours, and then PBS (pH7 .2) Rinse for 15 min, twice; rinse with sterile water for 15 min, twi...

Embodiment 2

[0043] A kind of soft tissue repair biomaterial, its preparation method comprises the steps:

[0044] (1) Dissolving type I collagen with 0.5M acetic acid solution to obtain 1w / v% type I collagen acetic acid solution;

[0045] (2) Take a 10cm proximal jejunum from a healthy adult pig, remove excess connective tissue and residual impurities in the intestine, then place the tissue in a refrigerator at 4°C for 6 hours, and then soak it in 1.0N sodium chloride saline for 12 hours to remove intestinal epithelial cells ; After that, the serosa and smooth muscle tissue were removed by mechanical scraping and hydration to obtain small intestinal submucosa tissue;

[0046] (3) Place the small intestinal submucosa tissue prepared in step (2) in a mixed solution of peracetic acid and ethanol (the volume fraction of oxyacetic acid is 0.1%, and the volume fraction of ethanol is 4%) to soak for 2 hours, and then PBS (pH7 .4) Rinse for 15 min, twice; rinse with sterile water for 15 min, twi...

Embodiment 3

[0052] A kind of soft tissue repair biomaterial, its preparation method comprises the steps:

[0053] (1) Dissolving type I collagen with 0.5M acetic acid solution to obtain 1w / v% type I collagen acetic acid solution;

[0054] (2) Take a 10cm proximal jejunum from a healthy adult pig, remove excess connective tissue and residual impurities in the intestine, then place the tissue in a refrigerator at 4°C for 6 hours, and then soak it in 1.0N sodium chloride saline for 12 hours to remove intestinal epithelial cells ; After that, the serosa and smooth muscle tissue were removed by mechanical scraping and hydration to obtain small intestinal submucosa tissue;

[0055] (3) Place the small intestinal submucosa tissue prepared in step (2) in a mixed solution of peracetic acid and ethanol (the volume fraction of oxyacetic acid is 0.1%, and the volume fraction of ethanol is 4%) to soak for 2 hours, and then PBS (pH7 .4) Rinse for 15 min, twice; rinse with sterile water for 15 min, twi...

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Abstract

The invention discloses a soft tissue repair biological material. The preparation method of the biological material comprises the following steps: preparing an I-type collagen acetic acid solution; preparing small intestine submucosa tissues; preparing small intestine submucosa tissue powder; preparing a small intestine submucosa acellular matrix lysate solution; preparing a primary freeze-dried stent; and preparing the soft tissue repair biological material. The adjustability of the structure and the mechanical property of the synthetic material can be realized at the same time, and a material capable of inducing tissue repair and meeting the individual requirements of clinical material properties is provided for soft tissue repair.

Description

technical field [0001] The invention relates to the technical field of medical engineering, in particular to a soft tissue repair biological material and a preparation method thereof. Background technique [0002] Soft tissue tearing and loss caused by trauma, organ and tissue defects caused by surgery, skin and subcutaneous tissue necrosis caused by burns and scalds are a class of major diseases that seriously endanger human health. Due to the lack of donor sources for organ and tissue transplantation and the high cost of surgery, the treatment of such diseases is very difficult. With the rapid development of biomedicine, biomaterials have shown excellent application prospects in the field of tissue defect repair and regeneration, mainly through the regulation of the damaged tissue microenvironment to promote tissue repair. [0003] In the past 20 years, the research on biomaterials for tissue repair and the development of related clinical technologies have accelerated sig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/36A61L27/50A61L27/56A61L27/58A61F2/02A61F2/08A61F2/10
CPCA61L27/24A61L27/3629A61L27/3633A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L27/56A61L27/58A61F2/02A61F2/08A61F2/10A61F2/105A61L2430/34A61L2300/412A61L2430/40A61L2300/602A61F2240/001A61F2002/0894
Inventor 阚鹏
Owner JIANGYIN PEOPLES HOSPITAL
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