Method for reducing total mercury content in rice

A rice and strain technology, applied in microorganism-based methods, horticultural methods, rice cultivation, etc., can solve the problems of complex preparation of sodium selenite-modified biochar, damage to soil ecosystems, and high energy consumption for biochar preparation. , to achieve the effect of less workload, simple spraying operation and less environmental damage

Active Publication Date: 2021-08-27
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventive method can effectively reduce the absorption of inorganic mercury and methylmercury by rice, but the disadvantage of this method is that the preparation of sodium selenite modified biochar is relatively complicated, the cost is high, and it is not environmentally friendly, and sodium selenite is highly toxic , which may harm the soil ecosystem
This method can reduce the absorption of mercury by crops, but the disadvantage of this method is that the effect of non-fine treatment in the field is not clear, and this soil conditioner needs to be added every time rice is planted, and long-term addition of mineral materials will change the soil structure. risks o

Method used

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  • Method for reducing total mercury content in rice
  • Method for reducing total mercury content in rice
  • Method for reducing total mercury content in rice

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1 Detection of a bacteria Pseudomonas taiwanensis used in the present invention

[0031] (1) form

[0032] The bacteria used in the present invention, Pseudomonas Taiwanensis microscope (1000x) observed photos figure 1 , Flat-plate colonies figure 2 .

[0033] (2) 16S rRNA gene sequence analysis

[0034] 116S rRNA gene sequence

[0035]

[0036]

[0037] 216S rRNA gene sequencing BLAST results

[0038] The 16S rRNA gene sequencing BLAST results are shown in Table 1.

[0039] Table 1 Sequencing of the bacteria Pseudomonas Taiwanensis 16S rRNA gene of this invention Sequencing BLAST results

[0040]

[0041]

[0042]

[0043]

[0044]

[0045] 3 evolution tree

[0046] Evolutionary tree image 3 .

[0047] (3) Physiological and biochemical properties of the bacteria Pseudomonas taiwanensis used in the present invention

[0048] The results of physiological biochemical characteristics of the bacteria Pseudomonas TAIWANENSIS of the present invention are sho...

Example Embodiment

[0055] Example 2P11 Treatment of Water Mercury Treatment Water Supply Mercury

[0056] (1) Analysis of mercury velocity test kit:

[0057] 1) Test steps:

[0058] 1 Preparation medium: formulated 10 times of trypsin 胨 soy meat soup medium, then add HGCl to culture fluid 2 Make HG 2+ The final concentration is 1 mg / L;

[0059] The activation of 2P11 strains: Take the preserved P11 strain (activated P11 bacterial liquid ABS600 (absorbance value at 600 nm) at 0.4-0.5, and mixed with 30% glycerol is mixed to be the preserved P11 strain, P11 strains are stored below -20 ° C below -20 ° C, inoculated from 10x trypsin soy meat soup culture, in accordance with the volume ratio of 1: 100, cultured from 30 to 12 hours at 30 ° C, 180 rpm oscillating shaker, ie The bacterial liquid ABS 600 is between 0.4-0.5, i.e., the activation of the activation of the P11.

[0060] 3 Culture: In accordance with the volume of activation of the above-mentioned P11 in the mercury-containing medium formulate...

Example Embodiment

[0072] Embodiment 3 The method of reducing total mercury in rice in rice

[0073] 1) Test steps:

[0074] 1 Packet: The mercury pollution soil is divided into three groups, and the group A is a blank control group. The group B is the P11 culture liquid treatment group. The C group is a microorganism organic fertilizer (Millennium Fruit Stalk Streptomyces, Aquacine, Bacillus, Bacillus , Bacillus, Acidaliferia, Pseudomonas, Pseudomonas aerugini, and other fermentation were then treated with a P11 culture solution.

[0075] 2 Rice planting: Pack the soil according to the group in the basin, and the c group applied 150 g / kg (soil) step 1, the microorganism organic fertilizer, then watering all the pots, no soil, then cultivate The seedlings in about 13 days are inserted.

[0076] 3P11 Culture Preparation: The activated bacterial liquid of P11 of Example 2 was inoculated in a volume ratio of 1: 100 in a modified M9 medium (6 g / L of hydrogen phosphate 6 g / L, phosphobic dihydrogen ...

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Abstract

The invention discloses a method for reducing total mercury content in rice. The method includes the following steps of preparing a unique strain P11 culture solution, treating rice with P11, applying a compound fertilizer in a tillering stage and a heading stage respectively, performing daily management and the like. By adopting the method for reducing the total mercury content in the rice to plant the rice, the total mercury content in the rice can be obviously reduced; the rice planting production process is relatively simple, only one leaf surface spraying process is added compared with a conventional rice planting method, spraying operation is easy, the workload is small, and the labor cost and the economic cost are low; a used strain comes from a soil environment, the raw materials of a culture medium are simple and easy to obtain, and the culture solution is simple in preparation process, low in energy consumption and low in cost; and the sprayed culture solution is high in environmental safety and small in environmental harm, hardly causes secondary pollution, hardly changes a soil matrix and hardly affects a soil structure.

Description

technical field [0001] The invention relates to the technical field of crop safety production, in particular to a method for reducing total mercury in rice. Background technique [0002] With the development of my country's industrialization, the problem of environmental pollution is becoming more and more serious. In 2014, the Ministry of Environmental Protection and the Ministry of Land and Resources jointly released a survey on the status of soil pollution in the country, pointing out that the total exceeding rate of all pollutants was 16.1%, among which the top The third inorganic pollutants are Cd, Hg, and As in sequence. Among them, mercury damages the central nervous system and has adverse effects on the mouth, mucous membranes and teeth. Mercury can accumulate in the body and is easily absorbed by the skin, respiratory tract and digestive tract. Prolonged exposure to high mercury environments can lead to brain damage and death. [0003] Since mercury has long-range t...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01G7/06C12N1/20C12R1/38
CPCA01G22/22A01G7/06C12N1/20
Inventor 潘继承夏清强汪劲松张新潮万进陈彪彪
Owner HUBEI NORMAL UNIV
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