Supercharge Your Innovation With Domain-Expert AI Agents!

Bacterial agent containing bacillus velezensis SUNO-18S-36 and use thereof

A technology of SUNO-18S-36, Bacillus velesi, applied in application, fungicide, bacteria and other directions, can solve the problems of lack of efficient microbial inoculants, lack of microorganisms, etc.

Active Publication Date: 2021-08-31
苏农(广德)生物科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is a lack of microorganisms that can simultaneously decompose phosphorus, degrade pesticide residues, and have an antagonistic effect on plant pathogenic bacteria, and there is even a lack of high-efficiency bacterial agents for such microorganisms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial agent containing bacillus velezensis SUNO-18S-36 and use thereof
  • Bacterial agent containing bacillus velezensis SUNO-18S-36 and use thereof
  • Bacterial agent containing bacillus velezensis SUNO-18S-36 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 strain screening and identification

[0039] 1. Isolation and screening of bacterial strains.

[0040] 1. Materials and methods

[0041] 1.1 Sample

[0042] 2 diseased tomato plants and 3 rhizosphere soil samples in general fields;

[0043] 2 diseased watermelon plants and 3 rhizosphere soil samples in general fields;

[0044] 3 tomato diseased plants and 5 rhizosphere soil samples in the salinized field;

[0045] A total of 18 samples.

[0046] 1.2 Medium

[0047] LB liquid medium: 10 g of peptone, 5 g of yeast extract, and 10 g of sodium chloride were dissolved in water, and the volume was adjusted to 1 L, and the pH was adjusted to 7.0 to obtain LB liquid medium. Add 15 g of agar powder to the above LB liquid medium to obtain LB solid medium.

[0048] PDA liquid medium: Add 800mL of water to 200g of peeled potatoes and cut into small pieces, heat to boiling, keep for 20-30min, filter while hot with gauze, make up the volume of filtrate to 1000mL with...

Embodiment 2

[0090] The property of embodiment 2 Bacillus velezensis (Bacillus velezensis) SUNO-18S-36 strain

[0091] 1. Tolerance to acidity, alkalinity and salinity

[0092] 1. Materials and methods

[0093] 1.1 Activation of the SUNO-18S-36 strain: From the slant of the Bacillus velei SUNO-18S-36 strain, use a sterile inoculation needle to pick part of the bacterial lawn into a shake flask containing LB liquid medium (250mL shake flask Liquid volume 50mL), after culturing at 30°C and 150r / min for 24h, the LB medium became cloudy and was ready for use.

[0094] 1.2 Use LB medium as the base medium, adjust the pH with hydrochloric acid or NaOH aqueous solution to: 3, 4, 5, 6, 7 (control), 8, 9, 10, 11, and fill the above pH in a 250mL Erlenmeyer flask 50 mL of culture medium, and each pH test was repeated 3 times. After sterilizing the medium at each pH, ​​cool it down to room temperature. If the pH changes due to sterilization, adjust the pH to the value close to the value before ste...

Embodiment 3

[0150] The preparation of embodiment 3 Bacillus velezensis (Bacillus velezensis) SUNO-18S-36 strain inoculum

[0151] This example introduces the preparation of Bacillus velei SUNO-18S-36 bacterial agent.

[0152] 1. Preparation of fermented liquid of Bacillus velei SUNO-18S-36 strain with high live bacteria content and high spore rate

[0153] Activation of Bacillus Velez SUNO-18S-36 strain: From the slant of Bacillus Velez SUNO-18S-36 strain, use a sterile inoculation needle to pick part of the bacterial lawn into a shake flask filled with LB liquid medium Medium (50ml of liquid in a 250mL shake flask), after culturing at 30°C and 150r / min for 24h, the LB medium became turbid. Take the bacterial liquid and streak it into LB solid medium for 30 hours to obtain the activated Bacillus velei SUNO-18S-36 strain.

[0154] Preparation of seed liquid: the filling volume of LB liquid medium in the 500 mL seed shake flask is 100 ml. The activated single colony on the LB solid mediu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fermentation method of a bacillus velezensis strain SUNO-18S-36 and relates to the field of microorganisms. According to the fermentation method, a culture medium is adopted and comprises 4-10 g / L of glucose, 5-20 g / L of corn steep liquor, 5-25 g / L of yeast powder, 5-10 g / L of silkworm chrysalis meal, 0-10 g / L of calcium carbonate, 0-30 g / L of calcium gluconate, 0.1-1 g / L of magnesium sulfate heptahydrate, 0-0.1 g / L of manganese sulfate and 0-10 g / L of calcium phosphate, and the pH is 7.0-7.5. The microorganisms involved in the fermentation method have functions of dissolving phosphorus and degrading pesticide residues and have an antagonistic effect on pathogenic bacteria. The fermentation method can obtain fermentation liquor with high viable bacterium content, a high spore rate and a high bacteriostatic active substance within a relatively short time.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a bacterial preparation containing Bacillus velei SUNO-18S-36 and its application. Background technique [0002] In the prior art, some people reported the application of Bacillus Veles in the degradation of straw and sewage ammonia nitrogen, and some reported its application in the remediation of aluminum-toxic soil, alleviating aluminum-toxic stress and improving the ability of plants to resist aluminum-toxic stress. Bacillus Velez can control peanut root rot. However, in the prior art, there is a lack of microorganisms that can simultaneously decompose phosphorus, degrade pesticide residues, and have an antagonistic effect on plant pathogenic bacteria, and there is even a lack of high-efficiency bacterial agents for such microorganisms. Contents of the invention [0003] The object of the present invention is to provide a bacterial agent containing Bacillus velezensis ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N11/14A01N63/22A01P1/00A01P3/00A01P21/00A01C1/08A01G13/00C12R1/07
CPCC12N1/20C12N11/14A01N63/22A01C1/08A01G13/00
Inventor 王晓军邹玲玲李勇丰超王玲丽汤晓娟
Owner 苏农(广德)生物科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com