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Method for preparing monoglyceride by enzyme method

A technology for monoglyceride and enzymatic preparation, applied in the field of oil processing, can solve the problems of unfriendly tert-butanol, increase product safety hazards, and increase the difficulty of product separation by tert-butanol, and achieve good operational stability, bottom The effect of high conversion rate and product generation rate and good industrial application prospects

Active Publication Date: 2021-10-08
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Zeng et al. (Zeng et al., J Am Oil Chem Soc, 2010, 87:531-537) used Novozym 435 as a catalyst to catalyze the glycerolysis of camellia oil to prepare monoglycerides in the tert-butanol system. After 10 hours of reaction, the product contained The monoglyceride content reached 82%; although the addition of tert-butanol significantly improved the mutual solubility and mass transfer effect of the substrate, the conversion rate of the substrate and the yield of monoglyceride were higher, but the use of tert-butanol increased the separation efficiency of the product. Difficulty, and the introduction of tert-butanol is not only unfriendly to the environment, but also increases the safety hazard of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take by weighing the mixture of 100g deep eutectic solvent (choline chloride: glycerol=1:2, mol ratio) and soybean oil (the mol ratio of soybean oil and glycerin is 1:5) and place in the 500mL round bottom flask, stir to make Mix well, heat to 50°C, add 2g of Novozym 435 to it, react for 12 hours, centrifuge to separate the layers, and separate the upper phase, which is the reaction mixture. Adopt high performance liquid chromatography analysis product to form, contain 0.55% triglyceride, 9.34% diglyceride and 90.11% monoglyceride in the product; ℃, the feed flow rate is 1.5mL / min, and the vacuum degree of the separation column is 2.3Pa), the reaction product is separated and purified, and the monoglyceride content in the finally obtained product is 98.34%. The above-mentioned deep eutectic solvent (after supplementing glycerol) and immobilized enzyme can be used continuously for 100 times without significant decrease in monoglyceride yield and immobilized enzyme activi...

Embodiment 2

[0031] Take by weighing the mixture of 100g deep eutectic solvent (choline chloride: glycerol=1:2, mol ratio) and soybean oil (the mol ratio of soybean oil and glycerin is 1:8) and place in the 500mL round bottom flask, stir to make Mix well, heat to 70°C, add 10g of Novozym 435 to it, react for 4 hours, centrifuge to separate the layers, and separate the upper phase, which is the reaction mixture. Adopt high performance liquid chromatography analysis product to form, contain 0.45% triglyceride, 9.21% diglyceride and 90.34% monoglyceride in the product; ℃, the feed flow rate is 2.0mL / min, and the vacuum degree of the separation column is 5.3Pa), the reaction product is separated and purified, and the monoglyceride content in the finally obtained product is 98.39%. The above-mentioned deep eutectic solvent (after supplementing glycerol) and immobilized enzyme can be used continuously for 100 times without significant decrease in monoglyceride yield and immobilized enzyme activi...

Embodiment 3

[0033] Take by weighing the mixture of 100g deep eutectic solvent (choline chloride: glycerol=1:2, mol ratio) and soybean oil (the mol ratio of soybean oil and glycerol is 1:6) and place in the 500mL round bottom flask, stir to make Mix well, heat to 60°C, add 5g Lipozyme 435 to it, react for 6 hours, centrifuge to separate the layers, and separate the upper phase, which is the reaction mixture. Adopt high performance liquid chromatography analysis product to form, contain 0.51% triglyceride, 9.47% diglyceride and 90.02% monoglyceride in the product; ℃, the feed flow rate is 2.0mL / min, and the vacuum degree of the separation column is 0.5Pa), the reaction product is separated and purified, and the monoglyceride content in the finally obtained product is 98.03%. The above-mentioned deep eutectic solvent (after supplementing glycerol) and immobilized enzyme can be used continuously for 100 times without significant decrease in monoglyceride yield and immobilized enzyme activity....

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Abstract

The invention discloses a method for preparing monoglyceride by an enzyme method, and belongs to the technical field of enzyme separation and application. According to the method, a choline chloride-glyceryl eutectic solvent is used as a solvent and a substrate, under a condition that glycerol in a system is much excessive, lipase is adopted to carry out catalysis on oil and fat to realize glycerolysis to prepare the monoglyceride, and after reaction is finished, the monoglyceride is separated from an upper-layer oil phase. Compared with a traditional method of carrying out enzymatic glycerolysis reaction in an organic solvent system, the method for preparing the monoglyceride through enzymatic glycerolysis under the eutectic solvent system has the following advantages: 1) due to the fact that the reaction byproduct glycerol can be absorbed by the eutectic solvent, product inhibition is relieved, and a substrate conversion rate and a product generation rate are high; 2) the method is green and environment-friendly; 3) the product is likely to layer and can be easily separated after the reaction; and 4) since the system has a protective effect for the immobilized enzyme, the immobilized enzyme and the eutectic solvent can be repeatedly utilized. The method has good social, ecological and economic benefits and has a good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of oil processing, and in particular relates to a method for enzymatically preparing monoglycerides. Background technique [0002] Monoacylglycerol (MAG) is the most widely used nonionic emulsifier in the food industry, and its consumption accounts for more than 2 / 3 of the global emulsifier consumption market. As an excellent W / O emulsifier, monoglycerides are widely used in the fields of food, medicine and cosmetics. At present, in the industry, inorganic bases are used as catalysts to catalyze the glycerololysis of oils and fats to prepare monoglycerides at high temperatures (220-250 ° C). This process has problems such as high energy consumption, many by-products, and dark color of the product, which is green and harmonious with the current society. The conflict of sustainable development concept is becoming more and more prominent. Enzymatic glycerol hydrolysis to prepare monoglycerides has the charact...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 李道明钟小荣周端蒲华寅刘宁
Owner SHAANXI UNIV OF SCI & TECH