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Nano fluorescent probe as well as preparation method and application thereof

A nano-fluorescent probe and fluorescence intensity technology, which is applied in the fields of chemistry and medicine, can solve problems such as no public and reliable solutions, and achieve the effects of fast detection speed, strong pH stability, and good water solubility

Pending Publication Date: 2021-10-26
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is necessary to develop a new uric acid detection method based on fluorescence method, but there is no public and reliable solution

Method used

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  • Nano fluorescent probe as well as preparation method and application thereof
  • Nano fluorescent probe as well as preparation method and application thereof
  • Nano fluorescent probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This embodiment provides a nano fluorescent probe, which is prepared by the following method:

[0053] 1) Disperse 0.3 g of ascorbic acid powder in a mixed solvent of 20 mL of ethanol and water, and ultrasonically treat it for 5 min, wherein the volume ratio of ethanol and water is 1:1;

[0054] 2) Transfer the mixture obtained in step 1) to a 50mL tetrafluoroethylene-lined stainless steel autoclave, and calcinate at 120°C for 10h. After the reaction is completed, a clear brown aqueous solution is obtained, which is naturally cooled to room temperature;

[0055] 3) Filter the mixed solution obtained in step 2) through a 0.22 μm syringe, then dialyze with a dialysis bag with a molecular weight cut-off of 1000D for 6 hours, collect the dialysate, freeze-dry and concentrate to obtain carbon dots (CDs), that is, the nano-fluorescent probe , stored at 4°C.

[0056] The performance detection of the prepared carbon dots (nano fluorescent probes) is performed as follows.

[0...

Embodiment 2

[0068] This embodiment provides an application of the nano fluorescent probe of embodiment 1, which is used to detect the concentration of uric acid, specifically comprising the following steps:

[0069] S1. Establish a standard curve to characterize the relationship between uric acid concentration and fluorescence intensity:

[0070] S1-1, using ultrapure water to configure the nano-fluorescent probe into a nano-fluorescent probe solution with a concentration of 6 mg / mL;

[0071] S1-2. Take several centrifuge tubes, add 10uL urate oxidase with an enzyme activity of 4U / mL to each centrifuge tube, and then add 10, 50, 100, 200, 250, 400, 500, 1000 μL of urate oxidase at a concentration of 340μM uric acid aqueous solution, each centrifuge tube was dilute to 1mL with ultrapure water, and reacted at 40°C in the dark for 60min;

[0072] S1-3. First add 100 μL of the nano-fluorescent probe solution with a concentration of 6 mg / mL to each part of the uric acid standard solution, the...

Embodiment 3

[0081] This embodiment provides an application of the nanometer fluorescent probe of Embodiment 1, which is also used to detect the concentration of hydrogen peroxide or active oxygen.

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Abstract

The invention discloses a nano fluorescent probe as well as a preparation method and application thereof. The nano fluorescent probe is prepared by the following method of taking ascorbic acid as a precursor, taking water and ethanol as solvents, and adopting a hydrothermal method. The nano fluorescent probe is used for detecting uric acid. The invention provides a carbon quantum dot (carbon dot for short) for uric acid detection, and discloses a specific uric acid detection method, which combines a Fenton reaction catalyzed by ferrous ions to catalyze decomposition of hydrogen peroxide generated after uric acid oxidation to generate reactive oxygen species, so that the fluorescence intensity of the carbon quantum dot is quenched, and the effective detection of uric acid concentration is thus realized. The method has the advantages of simplicity and convenience in operation, low reagent dosage, high detection speed, high accuracy, good selectivity and the like; the carbon dots have the advantages of being simple in preparation method, good in water solubility, high in pH stability and the like, and large-scale production can be achieved.

Description

technical field [0001] The invention relates to the fields of chemistry and medicine, in particular to a nano fluorescent probe, its preparation method and application. Background technique [0002] Uric acid (uric acid, UA) is the end product of the metabolism of purine compounds, and blood uric acid is mainly excreted from the kidneys. Blood uric acid at normal concentrations can participate in the body's antioxidant process and has a synergistic effect with ascorbic acid; changes in blood uric acid content can fully reflect the status of human metabolism, immunity and other functions, and its quantitative detection is of great clinical significance. [0003] At present, the existing detection methods of uric acid mainly include ultraviolet spectrophotometry, double enzyme coupling method, liquid chromatography, chemiluminescence method, mass spectrometry and so on. These methods either need expensive instrument support, or the operation is cumbersome, or the detection ac...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/6428G01N2021/6432C01P2004/04C01P2002/82C01P2004/32C01P2004/64Y02A50/30
Inventor 李力董文飞梅茜葛明锋常智敏从瑛哥庞欣佩
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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