Enzyme composition and method for synthesizing Pro-xylane by chemical enzyme method

Abstract

The invention provides a method for synthesizing Pro-xylane by a chemical enzyme method. The method comprises the following steps: S1) mixing acetylacetone or acetoacetate compounds, xylose and an alkaline substance in a solvent, heating and reacting to obtain a first intermediate product; and S2) mixing the first intermediate product, dehydrogenase, coenzyme, a reducing agent and isopropyl alcohol in a phosphate buffer solution, and reacting to obtain the Pro-xylane. Compared with the prior art, the method has advantages that a chemical-enzyme method is used, advantages of chemical synthesis and enzyme catalysis are exerted, Pro-xylane is obtained through stereospecific reduction, pollution and dangerous chemical combination reagents are avoided, greenness of Pro-xylane production and manufacturing process is greatly improved, meanwhile, the product separation and purification are simple and efficient, defects that an existing route is low in stereoselectivity, large-scale production is difficult to achieve, and many hazardous wastes exist are overcome, and production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of organic synthesis, and in particular relates to an enzyme composition and a method for synthesizing boson by chemical enzymatic method. Background technique [0002] Boseine is a xylose derivative with cosmetic anti-wrinkle effect discovered by L'Oreal for many years. It is converted from xylose and has a wide range of biological activities. Studies have shown that it can rebuild the cell structure by promoting the formation of intercellular mucopolysaccharides, enhance the connection between the dermis and the epidermis, and improve the binding ability of the dermis and the epidermis, thereby achieving the effect of beauty and anti-wrinkle. [0003] In 2002, L'Oreal's international patent number WO02 / 051828Al named Nouveaux dérivés C-glycosides et utilization (new carbon sugar anhydride and its application) was authorized, which disclosed the original research and preparation of Boseine for the first tim...

AI Technical Summary

Problems solved by technology

First, the stereoselectivity of reducing the ketone carbonyl group is poor, and a pair of diastereoisomers is obtained, which is very difficult to separate

Second, the existing routes often require the use of dangerous reagents and processes such as sodium borohydride, lithium aluminum hydride, and catalytic hydrogenation, which introduce a large amount of pollutants

The 3rd, the by-product that these reagents produce simultaneously makes the separation and purification of product very difficult, such as above-mentioned route uses sodium borohydride as reducing agent to reduce the ketone carbonyl in β-acetone xyloside 2, and by-product is a large amount of boric acid, and boric acid is extremely Bose, which is easy to bind with polyhydric groups, needs to be purified by column chromatography, which is not easy for mass production

[0007] The Chinese patent with the publication number CN111876452A discloses a method for preparing Bosene by using a biological enzyme one-pot method, but the method uses unidentified enzymes such as isopropanol dehydrogenase, Bosene synthase, and carbonyl reductase , unbelievable

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