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A gene orefla11 promoting wood formation and its application

A gene and wood technology, applied to the gene OreFLA11 that promotes the formation of wood and its application fields, can solve the problems of the small number of FLAs genes and poor plant traits, and achieve the effects of promoting growth, thickening, and hard wood material

Active Publication Date: 2022-04-22
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of FLAs genes in the above species is relatively small, and the traits of the plants are not good

Method used

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  • A gene orefla11 promoting wood formation and its application
  • A gene orefla11 promoting wood formation and its application
  • A gene orefla11 promoting wood formation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Identification and Phylogenetic Analysis of the OreFLAs Gene Family of Tianmu Ironwood

[0054] We first used the AtFLA1 protein sequence of Arabidopsis thaliana to find FLA homologous sequences in the Tianmu iron wood sequence database by Blastp method, then removed redundant genes and analyzed the conserved domains in the Pfam and SMART databases, and finally Thirty-five FLAs gene sequences were identified in Ironwood, named OreFLA1-OreFLA35. In order to further determine the phylogenetic relationship of the OreFLAs gene family, the ML phylogenetic tree was constructed by RAxML software by using the 35 OreFLAs gene sequences of T. thaliana and 21 AtFLAs gene sequences of Arabidopsis thaliana. The results showed that the members of the OreFLAs gene family of Ironwood tianmu were clustered into four subfamilies (I-IV), and the OreFLAs genes of Ironwood tianmu had obvious expansion in the second subfamily ( figure 1 ). In order to further elucidate the charac...

Embodiment 2

[0055] Example 2 Chromosomal location, collinearity analysis and expression pattern analysis of OreFLAs gene in different tissues

[0056] Tianmu Ironwood has 8 chromosomes in total, and 35 OreFLAs genes are located on 7 chromosomes, of which the third chromosome does not contain any OreFLAs genes, and the second chromosome has the largest number of OreFLAs genes, a total of 18, such as figure 2 shown. And most of the significantly expanded genes in subfamily II are located on the second chromosome, and these genes are repetitive genes, and there is a collinear relationship between them. OreFLA11 and OreFLA12 are located on the eighth chromosome, and there is also a collinear relationship between the OreFLA11 gene and the duplicate gene located on the second chromosome. Combined with phylogenetic tree, chromosome location and collinearity analysis, we speculated that these duplicated genes located on the second chromosome were generated by gene duplication of OreFLA11, and O...

Embodiment 3

[0058] Example 3 Cloning of Ironwood OreFLA11 Gene Sequence

[0059] Utilize the RNA extraction and separation reagent Trizol to extract the total RNA of the leaf tissue of Temodium ironwood. The specific method is: weigh 0.1 g of fresh ironwood leaf tissue, immediately place it in liquid nitrogen and grind it into powder, then add 1ml Trizol reagent, Mix well, inhale into a 1.5ml centrifuge tube, place at room temperature for 10min; centrifuge at 12000r / min at 4°C for 10min, take the supernatant into a new 1.5ml centrifuge tube; Shake for 15s, let stand at room temperature for 2-3min; centrifuge at 12000r / min at 4°C for 15min, take the supernatant into a new 1.5ml centrifuge tube, discard the middle and lower layers; add an equal volume of isopropanol, mix well, and let stand at room temperature for 10min ; 4°C, 12000r / min centrifuge for 10min to precipitate RNA, discard the supernatant; add 75% ethanol to wash the precipitate 1-2 times, 4°C, 12000r / min centrifuge 2min; dry t...

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Abstract

The invention belongs to the field of genetic engineering, and specifically provides a gene OreFLA11 that promotes wood formation, its nucleotide sequence is shown in SEQ ID No.1, and its amino acid sequence is shown in SEQ ID No.2; it is overexpressed in Populus Xinjiang OreFLA11 gene can significantly increase the content of lignin and xylose in the stem of Populus Xinjiang, thicken the thickness of xylem and the thickness of the secondary wall of xylem fiber cells, and enhance the tensile strength of the stem. This gene has important theoretical and practical significance for cultivating high-quality wood with denser structure and harder quality, and excellent tree species resistant to pests and diseases and wind and sand.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to a gene OreFLA11 for promoting wood formation and its application. Background technique [0002] Woody plants refer to strong plants in which the roots and stems grow thicker to form a large amount of xylem, and the cell walls are also mostly lignified. The plant has well-developed xylem, hard stems, and is perennial. Wood is a lignified tissue formed by woody plants through secondary growth, and it is a general term for secondary vascular tissues produced by the axial division and differentiation of vascular cambium, and wood plays a great supporting role in human life. According to the different properties and characteristics of wood, people use them in different ways. Poplar is one of the woody plants, and it is an economic tree species widely distributed in the world. Widely used in intensive cultivation. Compared with conventional hybrid breeding methods, molecular tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/84C12N1/21A01H5/00A01H5/04A01H6/00C07K14/415C12R1/01
CPCC07K14/415C12N15/8255C12N15/8245C12N15/8261C12N15/8205
Inventor 万东石牛智敏刘建全杨勇志吴桂莉
Owner LANZHOU UNIVERSITY
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