Antibacterial hemostatic porous microsphere based on sodium alginate and nanocrystalline cellulose

A technology of nanocrystalline cellulose and sodium alginate, which is applied in the fields of application, medical science, surgery, etc., can solve the problems that affect the biocompatibility of microspheres, do not have antibacterial properties, and strong cytotoxicity, so as to prevent wound infection, Good biocompatibility, effect of promoting hemostasis

Active Publication Date: 2022-01-11
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the existing microsphere materials have a good hemostatic effect, they do not have antibacterial properties. After hemostasis, due to the strong hydrophilic property of the microspheres, it is easy to cause bacterial growth and infection in the wound.
In addition, the currently prepared cellulose-based hemostatic microsphere materials use a large number of chemical cross-linking agents such as epichlorohydrin and trimethylolpropane, and the residue of the cross-linking agent is likely to cause strong cytotoxicity and affect the microsphere biophase. capacity

Method used

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  • Antibacterial hemostatic porous microsphere based on sodium alginate and nanocrystalline cellulose
  • Antibacterial hemostatic porous microsphere based on sodium alginate and nanocrystalline cellulose
  • Antibacterial hemostatic porous microsphere based on sodium alginate and nanocrystalline cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Oil phase: Add different amounts of Span 80 (2%, 3%, 4%, 5%, 6%) to 90g of liquid paraffin, stir at 700rpm, 45°C for 1h;

[0032] 2) Water phase: Sodium alginate and cellulose nanocrystals (different mass ratios of the two in the water phase: 1:1, 2:1, 3:1, 4:1, 5:1 and the sum of the two accounted for Water phase mass ratio: 0.5%, 1.0%, 1.5%, 2.0%, 2.5%) were dissolved in 30ml deionized water.

[0033] 3) Add the water phase dropwise into the oil phase (the weight ratio of the water phase to the oil phase is 1:3), stir at 800rpm for 2h, then add 6ml of calcium chloride solution (20wt%) dropwise, and stir at 800rpm for 4h; Wash 2 times with n-hexane, absolute ethanol, and deionized water, centrifuge to remove the supernatant, and freeze-dry to obtain SA / NCC porous microspheres; 100 mg of freeze-dried SA / NCC porous microspheres are dropped into 20 ml polylysine solution (6 mg / ml), stirred at 200rpm for 24h, centrifuged and freeze-dried to obtain SA / NCC@PL porous mic...

Embodiment 2

[0035] Measure the liquid absorption and porosity of different amounts of Span 80 (OP) prepared in Example 1, different amounts and different ratios of sodium alginate and cellulose nanocrystal SA / NCC@PL porous microspheres, see the relevant results figure 1 and figure 2 , to obtain two groups of microsphere preparation conditions with better ratio a (4% OP 3:1 1.5%); b (3% OP 5:1 1.5%).

[0036] Determination of the liquid absorption rate of porous microspheres: Weigh the lyophilized SA / NCC@PL porous microspheres as W1, soak them in a centrifuge tube containing deionized water to fully expand. Then use low-speed centrifugation to remove excess liquid, and weigh the wet porous microspheres, denoted as W2. Calculate the liquid absorption rate according to the formula:

[0037]

[0038] Determination of the porosity of the porous microspheres: Soak a certain weight of freeze-dried SA / NCC@PL porous microspheres in 4 mL of absolute ethanol, and oscillate ultrasonically for 1...

Embodiment 3

[0041] Comparing the coagulation index (BCI%) of different materials with the negative control group and the commercially available Languan brand chitosan hemostatic powder, the coagulation index of the b (3% OP 5:1 1.5%) group was finally obtained, and the coagulation effect was the best. Related results see image 3 .

[0042] Determination of coagulation index: 10 mg SA / NCC@PL porous microspheres were placed at the bottom of a glass bottle, and preheated at 37°C for 10 minutes. Then add 200ul of fresh anticoagulated rabbit blood and mix well, add 20ul of CaCl 2 The solution was incubated at 37°C for 10 min with mixing. Add 5ml of deionized water to the glass flask and vibrate (50rpm) for 2min to clean uncoagulated red blood cells. Measure the absorbance of the resulting solution at 540 nm with a UV spectrophotometer. The absorbance of 200ul uncoagulated whole blood in 5ml deionized water was used as a negative control. The BCI is calculated according to the following f...

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Abstract

The invention provides preparation of a antibacterial hemostatic porous microsphere with good biocompatibility, nontoxicity and biodegradability. The sodium alginate/cellulose nanocrystalline porous microsphere is prepared by using an inverse emulsion method. The antibacterial hemostatic porous microsphere of sodium alginate and cellulose nanocrystalline has simple preparation method, available materials, and good safety coefficient. The sodium alginate/nanocrystalline cellulose loaded epsilon-polylysine porous microsphere prepared by the method of the invention is applied in wound hemostasis to quickly absorb blood, reduce blood flow and promote local aggregation of red blood cells, blood coagulation factors and platelets at a wound, thereby promoting hemostasis. In addition, the antibacterial hemostatic porous microsphere of the present invention can prevent wound infection and promote tissue healing due to antibacterial performance.

Description

technical field [0001] The invention belongs to the field of polymer materials, in particular to an antibacterial and hemostatic porous microsphere based on sodium alginate and nanocrystalline cellulose. Background technique [0002] In recent years, a lot of hemostatic agents or hemostatic dressings have been developed at home and abroad, among which there is no lack of hemostatic dressings with antibacterial properties, but most of the products are made of antibacterial materials such as gold, silver, iodine, etc. particle. However, although the compounding of antibiotics can reduce the dosage of oral or injected antibiotics, the problem of drug resistance caused by long-term use of antibiotics is unavoidable; more importantly, hemostatic dressings compounded with antibacterial particles such as silver and iodine cannot be absorbed by the human body and Degradation, limited to the use of skin on the surface of the body, not suitable as a hemostatic dressing for internal u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00A61L24/02A61L24/04
CPCA61L24/08A61L24/046A61L24/02A61L24/0036A61L24/0042A61L24/0015A61L2400/12A61L2400/04A61L2300/232A61L2300/404A61L2300/418C08L5/04C08L1/04C08L77/04
Inventor 欧阳小琨赵丽娟凌俊红蒋方园杨立业
Owner ZHEJIANG OCEAN UNIV
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