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Preparation method and application of active bovine achilles tendon collagen peptide capable of whitening skin and fading spots

A technology of tendon collagen and bovine Achilles tendon, applied in the field of preparation of bovine Achilles tendon collagen peptide, can solve the problems of poor taste, fishy and bitter taste, etc., and achieve the effect of protecting gastric mucosa, excellent taste and high purity

Pending Publication Date: 2022-01-11
陕西医赛尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at technical problems such as fishy and bitter taste and poor taste in the application process of bovine Achilles tendon collagen peptide in the prior art, and there is no document or patent report that bovine Achilles tendon collagen peptide can inhibit the formation of stains

Method used

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  • Preparation method and application of active bovine achilles tendon collagen peptide capable of whitening skin and fading spots
  • Preparation method and application of active bovine achilles tendon collagen peptide capable of whitening skin and fading spots
  • Preparation method and application of active bovine achilles tendon collagen peptide capable of whitening skin and fading spots

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Preparation method of active bovine Achilles tendon collagen peptide for whitening and lightening spots

[0026] A preparation method for whitening and lightening active bovine Achilles tendon collagen peptide, comprising the steps of:

[0027] (1) Remove the soft tissue and fat from the fresh bovine Achilles tendon, wash it with purified water, crush it into 0.2mm×0.2mm tissue fragments with a pulverizer, add it to saline with a concentration of 5mol / L, stir and soak for 20 hours, Then wash it in warm water at 50°C, and then add NaHCO with a concentration of 10-15%. 3 Soak in the solution for 30 minutes, remove serum and other components, wash with purified water, add 5-10% sodium carbonate solution, soak for 1 hour, degrease, and then sterilize. After that, all steps are completed under aseptic operation.

[0028] (2) Add 0.5-2L of pepsin to the bovine Achilles tendon treated in step (1), and perform ultrasonic treatment to obtain collagen with a triple he...

Embodiment 2

[0031] Embodiment 2: The preparation method of whitening active bovine Achilles tendon collagen peptide

[0032] On the basis of the preparation method in Example 1, a method for preparing whitening and lightening active bovine Achilles tendon collagen peptide is provided. 3 Soak in the solution for 30 minutes, wash and then add 5% sodium carbonate solution to soak for 1 hour to degrease, add 0.5L pepsin to the processed beef Achilles tendon, and ultrasonicate. Centrifugal filtration, collect the filtrate, add a compound enzyme with a mass-volume ratio of 2%, and enzymatically hydrolyze it for 30 minutes to obtain active collagen peptides for whitening and lightening spots, wherein the compound enzyme is neutral protease and papain with a mass ratio of 1:2:1 and alkaline protease enzyme mix.

Embodiment 3

[0033] Embodiment 3: the preparation method of whitening active bovine Achilles tendon collagen peptide

[0034] On the basis of the preparation method in Example 1, a method for preparing whitening and lightening active bovine Achilles tendon collagen peptide is provided. 3 Soak in the solution for 30 minutes, wash and then add 6% sodium carbonate solution to soak for 1 hour to degrease, add 1L of pepsin to the processed bovine Achilles tendon, and perform ultrasonic treatment. Centrifugal filtration, collect the filtrate, add a compound enzyme with a mass-volume ratio of 2.5%, and enzymolyze it for 40 minutes to obtain active collagen peptides for whitening and lightening spots, wherein the compound enzyme is neutral protease and papain with a mass ratio of 1:2:1 and alkaline protease enzyme mix.

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Abstract

The invention discloses a preparation method and application of active bovine achilles tendon collagen peptide capable of whitening skin and fading spots. By improving the extraction process of the bovine achilles tendon collagen peptide, the bovine achilles tendon is hydrolyzed into the collagen peptide by mixed enzyme, and the collagen peptide with the molecular weight smaller than 3KD and the optimal tyrosinase inhibition effect is obtained through separation by an ultrafiltration method. The bovine achilles tendon collagen peptide obtained through process optimization is high in purity, small in molecular weight and easy to absorb. According to the preparation method, the advantages of the enzymolysis method and the ultrafiltration method are utilized, the extracted collagen peptide is high in activity and small in harm to a human body, and the technical problem that tyrosinase inhibitors such as benzenediol (HQ), arbutin, kojic acid, azelaic acid, L-ascorbic acid, ellagic acid and tranexamic acid have potential toxicity to the human body in the prior art is solved.

Description

technical field [0001] The present invention mainly relates to the technical field of health food, in particular, the present invention relates to the technical field of preparation method and application of bovine Achilles tendon collagen peptide with whitening and blemish-relieving activity. [0002] technical background [0003] Spots refer to spots that are different in color from the surrounding skin. Generally, age spots and freckles are common. There are many factors for its formation, and the formation process is also very complicated. It is related to age, endocrine, stress, emotion, pregnancy, insomnia, drugs, hormones, etc. , sun, wind, unhealthy lifestyle and many other factors. [0004] Melanin in the human body has the greatest influence on skin color. Melanin is usually produced by melanocytes in the basal layer of human skin. Tyrosine in melanocytes is oxidized under the action of tyrosinase to generate dopa and dopaquinone, and finally melanin. Tyrosinase i...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/34A61K38/39A61K9/08A61P17/00A61P1/04A61P17/18A61P37/02A61K8/65A61Q19/02A61Q19/00
CPCC07K14/78C12P21/06A61K38/39A61K9/0095A61P17/00A61P1/04A61P17/18A61P37/02A61K8/65A61Q19/02A61Q19/00A61K2800/782
Inventor 杨劲鹏谢晓燕
Owner 陕西医赛尔生物科技有限公司
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