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Preparation method and application of corn glutamine peptide with intestinal barrier protection function

A technology of glutamine peptide and protective function, which is applied in the field of preparation of corn glutamine peptide, can solve the problems of high price of chemical synthesis, low glutamine content, and difficult to completely remove organic reagents, etc., to relieve colon shortening, The effect of increasing the content and alleviating colitis in mice

Pending Publication Date: 2022-02-08
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical synthesis method has potential safety hazards such as high price and difficulty in completely removing organic reagents; although the reaction conditions for the preparation of glutamine peptides by enzymatic hydrolysis of gluten with protease are mild and no toxic and harmful components are formed, the content of glutamine in the enzymatic hydrolysis product is relatively low

Method used

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  • Preparation method and application of corn glutamine peptide with intestinal barrier protection function
  • Preparation method and application of corn glutamine peptide with intestinal barrier protection function
  • Preparation method and application of corn glutamine peptide with intestinal barrier protection function

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preparation example Construction

[0027] The invention provides a preparation method of corn glutamine peptide with intestinal barrier protection function, comprising the following preparation method:

[0028] using alkaline protease to carry out the first enzymolysis of zein to obtain the first enzymolysis solution;

[0029] The second enzymatic hydrolysis is carried out on the first enzymatic hydrolysis solution by using complex protease to obtain the second enzymatic hydrolysis solution and then dried to obtain corn glutamine peptide.

[0030] The present invention utilizes alkaline protease to carry out the first enzymolysis on zein to obtain the first enzymolysis solution. When performing the first enzymolysis in the present invention, the dosage of the alkaline protease is preferably 1000-1500 U / g zein, more preferably 1200-1450 U / g zein, more preferably 1200-1300 U / g zein. The temperature of the first enzymolysis in the present invention is preferably 60°C-70°C, more preferably 60-65°C; the time of the...

Embodiment 1

[0043] The preparation of corn glutamine peptide consists of the following steps:

[0044] (1) raw material pretreatment: adjust 200g corn gluten powder (its protein content is about 60%, wherein glutamine content accounts for about 8.3% of corn total protein) water content to 15%, utilize twin-screw extruder, at 170 Extrusion and puffing are carried out under the condition of ℃; after puffing, it is dried at room temperature, crushed and passed through a 60-mesh sieve to obtain 200 g of extruded corn gluten powder.

[0045] (2) Carry out destarch pretreatment to extruded corn gluten powder: with the 0.02mol / L phosphate buffer of pH 6.0, step (1) extruded corn gluten powder is mixed into the suspension that concentration is 10% (m / v) , adding α-amylase with 1% mass of extruded corn gluten powder, and hydrolyzing at 65° C. for 2 hours to remove starch. After the reaction, the enzymolysis solution was inactivated at 100°C for 20min, centrifuged at 8000r / min for 10min, the preci...

Embodiment 2

[0051] Same as Example 1, the difference lies in step (3) hydrolysis with alkaline protease for 2 hours.

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Abstract

The invention provides a preparation method of corn glutamine peptide with an intestinal barrier protection function, which comprises the following preparation steps: carrying out first enzymolysis on corn protein by using alkaline protease to obtain first enzymatic hydrolysate; and performing second enzymolysis on the first enzymatic hydrolysate by using compound protease to obtain a second enzymatic hydrolysate, and drying to obtain the corn glutamine peptide. Results of the embodiment show that the corn glutamine peptide can efficiently release glutamine in corn protein, and the release amount of the glutamine accounts for 75.00%-97.50% of that of the glutamine in the original corn protein. The invention can significantly improve weight loss of mice, reduce disease activity indexes, relieve colon shortening, reduce the content of proinflammatory factors IL-1beta, IL-6 and TNF-alpha in colon and increase the content of anti-inflammatory cytokine IL-10, and has a certain protection effect on intestinal barriers.

Description

technical field [0001] The invention belongs to the technical field of active peptide application, and in particular relates to a preparation method and application of corn glutamine peptide with intestinal barrier protection function. Background technique [0002] In recent years, various chronic diseases caused by the intestinal tract have shown a trend of frequent and high incidence. There are many factors that cause intestinal diseases, but the root cause is the problem of intestinal barrier function. Abnormal intestinal barrier function will lead to disordered digestion and absorption of nutrients, slow growth, decreased disease resistance, and increased susceptibility to pathogenic microorganisms, which in turn can cause various diseases such as colitis, irritable bowel inflammation, diarrhea, and constipation happened. [0003] Glutamine (Gln) is often used clinically as an enteral nutrient to protect the intestinal barrier, but its application is severely limited du...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/14A61K38/01A61P1/00A23L33/18
CPCC12P21/06C07K1/34C07K1/14A61K38/011A61P1/00A23L33/18A23V2002/00A23V2200/32A23V2250/55
Inventor 刘晓兰郑喜群景言李冠龙
Owner QIQIHAR UNIVERSITY