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Lycium barbarum polysaccharide and application thereof in reducing blood sugar

A combination technology of Lycium barbarum polysaccharides, applied in the field of natural active substance preparation, can solve problems such as allergies, immune overstimulation, and complex sugar chain connection forms

Active Publication Date: 2022-03-11
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight distribution of Lycium barbarum polysaccharides is wide and the sugar chain connection form is complex, resulting in a low content of Lycium barbarum polysaccharides that play a hypoglycemic effect. If you want to achieve the purpose of lowering blood sugar, you need to consume a large amount of Lycium barbarum extracts, which will lead to immune overstimulation, leading to allergies, etc. side effect

Method used

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  • Lycium barbarum polysaccharide and application thereof in reducing blood sugar
  • Lycium barbarum polysaccharide and application thereof in reducing blood sugar
  • Lycium barbarum polysaccharide and application thereof in reducing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of Lycium barbarum polysaccharide LICP007-S5 with potential hypoglycemic effect

[0028] 1. Preparation method of Lycium barbarum polysaccharide component LICP007-S5

[0029] 1.1 Instruments, reagents and materials

[0030] Lycium barbarum dried fruit, DEAE-52 cellulose, Sephadex G-50, monosaccharide standard (arabinose, ribose, xylose, glucose, fructose, mannose, galactose, fucose, rhamnose, galactobionic acid, glucuronic and mannonic acids). All other chemicals were at least analytical grade. The solvent for HPLC is chromatographically pure. Vacuum freeze dryer, ultraviolet spectrophotometer, electronic analytical balance, centrifuge, high shear dispersion emulsification, ultrafiltration membrane element, peristaltic pump, Malvern laser light scattering instrument and size exclusion chromatography combined system (SEC-MALLs ).

[0031] 1.2 Preparation of Lycium barbarum crude polysaccharide

[0032] figure 1 It is a flow chart of separati...

Embodiment 2

[0038] Example 2: Evaluation of hypoglycemic activity of different parts and components of Lycium barbarum polysaccharide (screening evaluation of α-glucosidase and α-amylase inhibitory activity).

[0039] 2.1. Evaluation of α-amylase inhibitory activity

[0040] The α-amylase inhibitory activity of LICP007, LICP008, LICP009 and LICP010 of four Lycium barbarum polysaccharide fractions was determined. Take 45 μL each of LICP007~LICP010 solutions (0.5, 1.0, 5.0, 10.0 and 20.0 mg / mL), add 15 μL α-amylase (0.02 mg / mL) and incubate in a water bath at 37°C for 10 min, then add 60 μL starch solution (0.08% ), react in a water bath at 37°C for 15 minutes, add 60 μL of hydrochloric acid solution (1 mol / L), stop the reaction, and then add 20 μL of iodine solution (0.01 mol / L) to obtain a reaction solution. At the same time, 15 μL of PBS buffer was used to replace the α-amylase solution as a blank. Finally, the absorbance was measured on a microplate reader at 630 nm. LICP007-N1, LICP...

Embodiment 3

[0052] Example 3: Structural analysis of Lycium barbarum polysaccharide LICP007-S5

[0053] 3.1 Molecular weight determination

[0054] The molecular weight of polysaccharides was determined by multi-angle laser light scattering coupled with size exclusion chromatography (SEC-MALLs). Such as Figure 4 As shown, the results show that LICP007-S5 is composed of two main peaks, Peak I and Peak II, and the number average molecular weight Mn, weight average molecular weight Mw and average molecular weight Mz of Peak I are 4.769×10 3 ,9.202×10 3 and 1.690×10 4 g / mol, the dispersibility index PDI is 2.002, the number average molecular weight Mn of Peak II, the weight average molecular weight Mw, and the average molecular weight Mz are 7.871×10 4 , 1.031×10 5 and 2.101×10 5 g / mol, the dispersibility index PDI is 1.310. Therefore, LICP007-S5 is a polydisperse heteropolysaccharide.

[0055] 3.2 Monosaccharide composition analysis

[0056] Monosaccharide composition analysis is a...

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Abstract

The invention provides lycium barbarum polysaccharide and application thereof in reducing blood sugar. The lycium barbarum polysaccharide is prepared by the following steps: separating a lycium barbarum water extract through an ultrafiltration membrane with the molecular weight of 30kDa, freeze-drying the intercepted lycium barbarum water extract component, dissolving the lycium barbarum water extract component in distilled water, removing protein and pigment, dialyzing, and freeze-drying; dissolving the dried product again for ion exchange resin column chromatography, eluting by using a sodium chloride solution, collecting components, dialyzing by using a 3500 Da dialysis bag, freeze-drying the dialysis product, dissolving in distilled water again, further separating by using sephadex, collecting the components, and freeze-drying to complete the preparation. The lycium barbarum polysaccharide provided by the invention can be used for preparing products for reducing blood sugar, including but not limited to functional foods, health foods, foods for special medical purposes or new medicines.

Description

technical field [0001] The invention belongs to the technical field of preparation of natural active substances, and in particular relates to a wolfberry polysaccharide and its application in lowering blood sugar. Background technique [0002] Diabetes mellitus is a common clinical metabolic disease, which is caused by insufficient insulin secretion or impaired biological effects, and its incidence is also closely related to genetic and environmental factors. The prevalence and incidence of diabetes have increased in recent decades, with approximately 10% of the population worldwide having or at risk of developing type 2 diabetes mellitus (T2DM). Currently, the most clinically effective and widely used chemical drugs for treating diabetes include sulfonylureas, thiazolidinediones, α-glucosidase inhibitors, and biguanides. However, since diabetes requires long-term medication, the side effects and toxicity of therapeutic drugs cannot be ignored. Therefore, developing a hypo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10A23L33/125
CPCC08B37/006C08B37/0003A61K31/715A61P3/10A23L33/125A23V2002/00A23V2200/328A23V2250/51
Inventor 刘建飞邸多隆刘妍妍
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI