Unlock instant, AI-driven research and patent intelligence for your innovation.

Electrochemical preparation method and application of framework nucleic acid

An electrochemical and framework technology, applied in the field of electrochemical preparation of framework nucleic acids, can solve the problems of expensive equipment, large equipment size and difficulty, and achieve the effects of easy standardization, complete framework structure and good dispersion

Active Publication Date: 2022-03-11
SHANGHAI JIAO TONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an electrochemical preparation method of framework nucleic acid and its application, so as to solve the problem of expensive equipment and large volume of equipment used in the process of preparing framework nucleic acid by existing thermal denaturation technology to precisely control the temperature of the system. And other issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical preparation method and application of framework nucleic acid
  • Electrochemical preparation method and application of framework nucleic acid
  • Electrochemical preparation method and application of framework nucleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The construction of embodiment 1 electrochemical device

[0049] In this embodiment, constructing an electrochemical device includes the following steps:

[0050] Preparation of iridium oxide electrode: use iridium wire as working electrode, silver / silver chloride electrode as reference electrode, platinum electrode as counter electrode, 0.7M Na 2 HPO 4 The solution is an electrolyte, and Chenhua CHI1040c is used to carry out cyclic voltammetric oxidation according to the parameters shown in Table 2. The iridium oxide electrode treated by cyclic voltammetry oxidation was soaked in water, and hydrated and aged for 24 hours.

[0051] Initial potential (V) -0.5 Maximum potential (V) 0.8 Lowest potential (V) -0.5 Final potential (V) 0 Sweep speed(V / s) 0.1 Scanning laps 300 Rest time (V) 0 Sensitivity (A / V) 10 -4

[0052] The performance test process of the electrode: the Britton-Robinson (BR) buffer solution wit...

Embodiment 2

[0056] Example 2 The regulation of solution pH on DNA denaturation and renaturation process

[0057] The measurement process of DNA double-strand Tm value under different pH conditions: Quantify the required single-stranded DNA with an ultraviolet-visible absorption spectrometer, and quantify the concentration of all DNA single-strands to a concentration of 100 μM. Mix two complementary single strands with chain lengths of 13 bp, 20bp, 26bp, 37bp and 47bp in equal proportions and add them to BR buffer solutions with different pHs containing 1× SYBR-Green to form a final concentration of the single strands. for 1 μM samples. Put the prepared samples into the RT-PCR instrument, heat at 95°C for 10min, then suddenly drop to 25°C, then slowly raise the temperature to 95°C, and collect the fluorescence signals of each group of samples during the whole temperature change process in real time.

[0058]Verification process of double-stranded DNA denaturation and renaturation in diffe...

Embodiment 3

[0061] Example 3 Preparation of DNA Tetrahedral Framework Nucleic Acids of Different Sizes

[0062] Experimental procedure for screening the salt concentration of framework nucleic acids prepared by electrochemical methods: for screening optimal Mg for DNA tetrahedral preparation 2+ concentration, the four single-stranded DNAs forming the DNA tetrahedral nanostructure were mixed in TM buffer and 10mM MgCl in equal proportions at a final concentration of 1 μM 2 , 20mM MgCl 2 , 40mM MgCl 2 and 60mM MgCl 2 Put it into a PCR instrument and heat it at 95°C for 10min, then quickly cool down to 4°C and maintain it for more than 10min. To screen the optimal Na for DNA tetrahedron preparation + Concentration, the four single-stranded DNAs forming the DNA tetrahedral nanostructure were mixed in equal proportions in TM buffer, 0.2M NaCl, 0.3M NaCl, 0.4M NaCl, 0.5 M NaCl and 0.6M NaCl at a final concentration of 1 μM, Put it into a PCR machine and heat it at 95°C for 10min, then quic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
correlation coefficientaaaaaaaaaa
Login to View More

Abstract

The invention provides an electrochemical preparation method and application of framework nucleic acid, and the method comprises the following steps: construction of an electrochemical device: the electrochemical device is formed by pasting a PDMS layer and a glass sheet layer and comprises a reaction zone and a control zone, silver / silver chloride is used as a working electrode, iridium oxide is used as a reference electrode, and iridium is used as a counter electrode; self-assembly of the framework nucleic acid: constructing the framework nucleic acid with designable morphology and biochemical properties through an electrochemical strategy, providing a required DNA single strand, adding the DNA single strand into a reaction region, and applying a certain potential to a working electrode so as to generate water oxidation reaction in the reaction region, reduce the pH value of a solution and generate DNA denaturation; and by applying another potential to the working electrode, a reduction reaction of water is carried out in the reaction zone, the pH value of the solution is increased, DNA renaturation is carried out, and electrochemical self-assembly of the framework nucleic acid is realized. The characteristic that the pH value of the solution can be quickly and accurately regulated and controlled by controlling the potential of the working electrode is utilized, so that a series of framework nucleic acids modified by specific functional groups in sizes are obtained.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to an electrochemical preparation method of framework nucleic acid and its application. Background technique [0002] DNA nanotechnology utilizes DNA double helix with precise structure and strict Watson-Click base pairing principle to realize precise two-dimensional and three-dimensional static or dynamic DNA nanostructures, which has broad application prospects in the fields of medical diagnosis and molecular machines. Among them, the construction of DNA nanostructures is mainly based on the principle of base complementarity between DNA single strands and the difference in binding energy between DNA single strands of different lengths and sequences to obtain the most stable configuration of DNA nanostructures. However, in single-strand DNA, due to some non-specific interactions, some secondary or tertiary structures (in a kinetic trap) are often produced, so DNA origami asse...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C25B3/07C25B3/09C12N15/10
CPCC25B3/07C25B3/09C12N15/10C12N2310/151C12Q2563/131C12Q2565/607Y02A50/30
Inventor 沈建磊李茜翟婷婷孙晨蕴樊春海
Owner SHANGHAI JIAO TONG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com