Bioactive glass nano-composite particle with antibacterial effect and disguised efficient hemostatic membrane-like structure and preparation method of bioactive glass nano-composite particle

A bioactive glass, antibacterial technology, applied in the field of biomedical materials, can solve the problems of no wound hemostasis effect, limited applicable wound types, single hemostasis mechanism, etc. The effect of cascade reactions

Active Publication Date: 2022-03-22
陕西立云至彩医疗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent 201810146389.9 discloses a mesoporous silica composite microsphere with high coagulation function, which improves its hemostatic performance by compounding with polysaccharides, but does not have antibacterial properties and has a general hemostatic effect
Chinese patent 201922184318.0 discloses a composite wound dressing with a bioactive glass base. By attaching an enzyme disinfectant to the bioactive glass layer, the composite dressing has an antibacterial function, but it does not have wound hemostatic effect and the applicable wound types are limited.
Ordinary macroporous bioactive glass has a general hemostatic effect and a single hemostatic mechanism. The membrane-like structure camouflage nanoparticles prepared by wrapping bovine serum albumin and chitosan through layer-by-layer self-assembly method can cooperate with multiple coagulation mechanisms to achieve rapid hemostasis At the same time inhibit the bacterial growth of the wound to achieve antibacterial and anti-inflammatory effect

Method used

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  • Bioactive glass nano-composite particle with antibacterial effect and disguised efficient hemostatic membrane-like structure and preparation method of bioactive glass nano-composite particle
  • Bioactive glass nano-composite particle with antibacterial effect and disguised efficient hemostatic membrane-like structure and preparation method of bioactive glass nano-composite particle
  • Bioactive glass nano-composite particle with antibacterial effect and disguised efficient hemostatic membrane-like structure and preparation method of bioactive glass nano-composite particle

Examples

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preparation example Construction

[0078] Preparation of macroporous bioactive glass: 1% surfactant cetyl ammonium bromide and 3.5% tetraethyl orthosilicate are dissolved in the mixed ammonia solution of ethanol and ether (water: ethanol: ether: ammonia water=150 :20:40:4), the pH value is 10-12, the temperature is controlled at 20-30°C, and stirred at 800-1000 rpm for 4 hours, the solution is gelled and then gelled to form a gel, the gel is 8000- Centrifuge at 12,000 rpm for 5 minutes to collect samples, dry in vacuum at 30-60°C for 12-24 hours to remove moisture from the prepared wet gel, grind thoroughly, and calcinate at 550°C for 5 hours to remove surfactants and form a macroporous structure.

[0079] Preparation of aminated macroporous bioactive glass: disperse 50% macroporous bioactive glass particles in isopropanol, add 3-aminopropyltriethoxysilane dropwise, the speed is 1 ml / min, 200-500 rpm Stir, cool and reflux for 24-36 hours, centrifuge at 8,000-12,000 rpm for 5 minutes to collect, wash with deioni...

Embodiment 1

[0106] This example relates to the synthesis of macroporous bioactive glass

[0107] Add 210mL of 9.5% ethanol and 19% diethyl ether mixed aqueous solution, add 2g of surfactant CTAB, add 4mL of ammonia water dropwise, adjust the pH value to 10-12, add 7.5mL of ethyl orthosilicate TEOS into the syringe pump, weigh 1.4g of Ca(NO 3 ) 2 4H 2 Add O to the above solution, stir at 20-30°C, 1000 rpm for 4 hours, centrifuge at 10,000 rpm for 5 minutes to collect the precipitate, wash with absolute ethanol and deionized water three times, and dry in vacuum for 24-36 hours Afterwards, it is fully ground and fired at 550°C for 5 hours to obtain a macroporous bioactive glass. The diameter size distribution is 400-700nm, the pore size distribution is 50-70nm, and the specific surface area is 242.84m 2 g -1 .

[0108] Scanning electron microscopy ((SEM, JSM-7500F, JEOL, Japan) and transmission electron microscopy (TEM, Talos F200X, FEI, USA) were used to observe the morphology and mac...

Embodiment 2

[0110] This example relates to the synthesis of macroporous bioactive glass

[0111] Add 210mL of 9.5% ethanol and 19% ethylene glycol ethyl ether mixed aqueous solution, add 2g of surfactant CTAB, add 4mL of ammonia water drop by drop, adjust the pH value to 10-12, add 7.5mL of ethyl orthosilicate TEOS to the syringe pump , weighed 1.4g of Ca(NO 3 ) 2 4H 2 Add O to the above solution, stir at 20-30°C, 1000 rpm for 4 hours, centrifuge at 10,000 rpm for 5 minutes to collect the precipitate, wash with absolute ethanol and deionized water three times, and dry in vacuum for 24-36 hours Afterwards, it is fully ground and fired at 550°C for 5 hours to obtain a macroporous bioactive glass. Its diameter size distribution is 300-400nm, pore size distribution is 20-40nm, specific surface area is 698.36m 2 g -1 .

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Abstract

The invention relates to an antibacterial efficient hemostatic membrane-like structure disguised bioactive glass nano-composite particle and a preparation method thereof. The bioactive glass nano-composite particle comprises aminated macroporous bioactive glass, bovine serum albumin and chitosan. The preparation method comprises the following steps: taking aminated macroporous bioactive glass, deionized water, bovine serum albumin and chitosan as raw materials, according to a layer-by-layer self-assembly sequence, and through an electrostatic adsorption principle, obtaining the bioactive glass nano composite particle with the disguised membrane-like structure. The preparation process is simple, and no secondary product is generated. The membrane structure disguised porous nano-particles are wrapped by bovine serum albumin with a platelet generation promoting effect and chitosan with an erythrocyte gathering and antibacterial effect, and the membrane structure disguised porous nano-particles can simultaneously activate endogenous and exogenous blood coagulation pathways, quickly form thrombus stopping and wound blocking on a wound surface, reduce the bleeding amount and shorten the bleeding time. The coagulation effect and the biocompatibility of the bioactive glass are improved, the antibacterial performance is also given to the bioactive glass, and the bioactive glass has huge clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a bioactive glass nanocomposite particle with an antibacterial effect and a high-efficiency hemostatic membrane structure camouflage and a preparation method. Background technique [0002] Powdered hemostatic granules have broad application prospects in incompressible wounds such as large open wounds, internal organ injuries, and body penetrating wounds. Hemorrhage is a serious accident that occurs in people's daily life or in war. According to statistics, about 30% of the mortality rate is caused by excessive blood loss when the body is seriously injured. More than 85% of deaths are caused by uncontrolled bleeding. Massive bleeding in the human body may lead to complications or death, and effective hemostasis is an important means to prevent traumatic death. [0003] Therefore, a high-efficiency hemostatic material suitable for irregular wounds such as limbs, trunk, and inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08A61L24/02A61L24/00
CPCA61L24/0042A61L24/0015A61L24/0094A61L24/02A61L24/108A61L24/08A61L2400/04A61L2300/624A61L2300/404C08L89/00
Inventor 卢婷利郑彩云
Owner 陕西立云至彩医疗生物科技有限公司
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