A fermented feed that improves the survival rate and growth rate of prawn culture
A technology for fermenting feed and growth rate, applied in animal feed, animal feed, bacteria used in food preparation, etc., to reduce early death, increase the type and quantity of dominant flora, and improve shrimp immunity
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Embodiment 1
[0020] Embodiment 1: the antibacterial test of starter
[0021] 1.1 Preparation of medium and starter
[0022] Liquid medium: Add 0.5 g peptone (Typtone), 0.1 g yeast extract (Yeast Extract), 0.001 g ferric phosphate tetrahydrate (FePO 4 • 4H 2 O), adjust the pH to 7.6-7.8, sterilize at high temperature and high pressure (121 °C, 20 min) in a high-pressure steam sterilizer, and store in a chromatographic cabinet at 4 °C for later use.
[0023] Solid medium: add 0.5 g peptone (Typtone), 0.1 g yeast extract (Yeast Extract), 0.001 g ferric phosphate tetrahydrate (FePO 4 • 4H 2 O), 2 g of agar (Agar Powder), after being sterilized by high temperature and high pressure (121°C, 20 min) in a high-pressure steam sterilizer, when the temperature of the medium drops to about 50°C, pour the plate in a sterile ultra-clean workbench , about 15 ml per plate, and store it in a chromatographic cabinet at 4 °C after drying.
[0024] Starter: The components of the starter include: 15%-20% ...
Embodiment 2
[0033] Embodiment 2 The laboratory cultivation test of prawns
[0034] Preparation of fermented feed: 0.1Kg of starter, 150kg of basic raw materials, 25kg of brown sugar, and 25kg of water, after mixing, stir evenly, and ferment in airtight conditions at room temperature for three days. The basic raw materials include: 15% of rapeseed cake, 50% of soybean meal, 20% of fish meal, 8% of bran and 7% of corn gluten meal.
[0035] 840 shrimp juveniles of Penaeus vannamei were randomly selected and divided into 6 water bodies with a depth of 0.4 m 3 In the indoor circular cement pool, there are 140 tails in each pool. They were raised for 15 days before formal group feeding, and samples were taken during the holding period to detect possible pathogens. The experiment set up a blank control group and a test group, and each group had 3 parallels. The experimental prawn seedlings were fed 3 times a day (8:00, 2:00 pm and 8:00 pm), and the feeding amount was 3% to 5% of the body weig...
Embodiment 3
[0039] Embodiment 3 Aquaculture large group breeding test
[0040] Fermented feed is made with embodiment 2.
[0041] This test was carried out in Suzhou City, Jiangsu Province. The test ponds were 4 shrimp greenhouse culture ponds, each with an area of 5 mu. The bottom quality and hydrological conditions of the ponds were exactly the same. No. 1 and No. 3 ponds were used as the test group, and the bio-fermented feed was continuously mixed with bait (see Table 2 for the feeding ratio of fermented feed and common feed); No. 2 and No. 4 ponds were used as the control group, and the common feed was fed throughout the process. During the whole test, the four shrimp ponds had the same conventional farming operation, the average temperature in the greenhouse was 23.8°C during the test, and the test period was 60 days. Shrimp seedlings with the same size and health status were selected for the test.
[0042] Measuring indicators: growth of shrimp seedlings: in the experiment, 5...
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