Lactococcus lactis jyll-60 and its application in preparing products for improving immunity
A technology of Lactococcus lactis and products, which is applied in the field of probiotics, can solve the problems of poor distinction of mass consumers and inconsistent demand for decoction pieces, etc., to enhance human immunity, strong intestinal colonization ability, and strong gastrointestinal tract survival rate effect
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Embodiment 1
[0024] Example 1 Screening and identification of Lactococcus lactis JYLL-60
[0025] 1. Screening of strains
[0026] 1. Bacteria source:
[0027] In July 2019, naturally fermented yogurt was collected from herdsmen's homes in Nagqu, Tibet, and Lactococcus lactis strains were isolated.
[0028] 2. MRS plate medium:
[0029] MRS plate medium includes the following components: peptone 10.0g, beef extract powder 5.0g, glucose 20.0g, Tween 801.0mL, dipotassium hydrogen phosphate heptahydrate 2.0g, sodium acetate trihydrate 5.0g, triammonium citrate 2.0g , 0.2 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate tetrahydrate, 15.0 g of agar, and 1000 mL of distilled water.
[0030] After mixing the above components, natural pH, after stirring evenly, sterilize at 121°C and 0.1MPa for 20min.
[0031] 3. Screening method:
[0032] (1) Take 1 mL of naturally fermented yogurt at Nagqu herdsmen's homes in Tibet and set aside.
[0033] (2) Take sterilized physiological ...
Embodiment 2
[0048] Example 2 Preparation of Lactococcus lactis JYLL-60 product
[0049] (1) The preserved Lactococcus lactis JYLL-60 was activated on MRS plate medium and cultured at 37°C for 24h;
[0050] (2) The activated Lactococcus lactis JYLL-60 was inoculated into MRS liquid medium, and cultured at 37°C for 24 hours to obtain bacterial liquid;
[0051] MRS liquid medium includes the following components: peptone 10.0g, beef meal 5.0g, glucose 20.0g, Tween 801.0mL, dipotassium hydrogen phosphate heptahydrate 2.0g, sodium acetate trihydrate 5.0g, triammonium citrate 2.0g, Magnesium sulfate heptahydrate 0.2g, manganese sulfate tetrahydrate 0.05g, distilled water 1000mL;
[0052] (3) After the bacterial liquid is centrifuged, the bacterial cells are collected, washed with sterile physiological saline, and then resuspended in 15wt% reconstituted skim milk to obtain a suspension;
[0053] (4) Adjust the concentration of the suspension to 1.0-2.0×10 10 cfu / mL to obtain bacterial suspens...
Embodiment 3
[0055] Example 3 Experiment of gastrointestinal digestive juice resistance of Lactococcus lactis JYLL-60
[0056] (1) Preparation of artificial simulated gastric juice: dilute with 9.5% hydrochloric acid and distilled water to pH 1.5, add 1.0 g of pepsin per 100 mL, and filter through a 0.22 μm sterile filter after mixing.
[0057] (2) Preparation of artificial simulated intestinal fluid: 6.8g of KH 2 PO 4 Dissolve in 500 mL of distilled water, add 3 g of bile salts and 10 g of trypsin, adjust the pH of the solution to 6.8 with 4 g / L NaOH solution, dilute to 1 L with distilled water, and filter with a 0.22 μm sterile filter after mixing. , now with the current use.
[0058] (3) Weigh several copies of the viable bacteria obtained in Example 2 to be 1.5×10 9 The product of cfu / g was taken as a sample, and each sample weighed 1g, transferred to a preheated test tube containing 9mL of simulated gastric juice / intestinal fluid, and treated at 37°C and 80r / min for 1h, 2h, 3h, and...
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