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Method for preparing sesame oligopeptide from water extraction method sesame oil byproduct

A technology of by-products and oligopeptides, applied in the biological field, can solve the problems of less raw material sources and low product yields, and achieve the effects of shortening enzymatic hydrolysis time, high purity, and saving production costs

Pending Publication Date: 2022-04-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has fewer sources of raw materials and lower product yields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] ①Double-enzyme synchronous enzymatic hydrolysis

[0032] Mix sesame meal and distilled water according to the material-to-liquid ratio of 1:25 (g:mL), adjust the pH to 9.5 with 1 mol / L sodium hydroxide solution, and use the enzyme activity unit of alkaline protease and papain at 1.5:1 (U: U), add alkaline protease and papain (food grade), add 7000 U per gram of sesame meal, 50°C dual-enzyme synchronous enzymatic hydrolysis for 3 h, and 95°C for 15 min to obtain enzymatic hydrolyzate;

[0033] The sesame meal is a by-product of preparing sesame oil by the water substitution method, with a water content of 67.3% and a protein content of 12.6%;

[0034] The simultaneous enzymatic hydrolysis of the dual enzymes is that alkaline protease and papain directly hydrolyze sesame meal;

[0035] The enzyme inactivation is to inactivate alkaline protease and papain at 95°C and 15 min;

[0036] ②Separation and purification of enzymatic hydrolyzate

[0037] Adjust the pH of the enz...

Embodiment 2

[0043] ①Double-enzyme synchronous enzymatic hydrolysis

[0044] Mix sesame meal and distilled water according to the material-to-liquid ratio of 1:30 (g:mL), adjust the pH to 9.0 with 1 mol / L sodium hydroxide solution, and use alkaline protease and papain in enzyme activity units of 1:1 (U: U), add alkaline protease and papain (food grade), add 6000 U per gram of sesame meal, 45°C dual-enzyme synchronous enzymatic hydrolysis for 2 h, and 95°C for 15 min to obtain enzymatic hydrolyzate;

[0045] The sesame meal is a by-product of preparing sesame oil by the water substitution method, with a water content of 67.3% and a protein content of 12.6%;

[0046] The simultaneous enzymatic hydrolysis of the dual enzymes is that alkaline protease and papain directly hydrolyze sesame meal;

[0047] The enzyme inactivation is to inactivate alkaline protease and papain at 95°C and 15 min;

[0048] ②Separation and purification of enzymatic hydrolyzate

[0049] Adjust the pH of the enzymati...

Embodiment 3

[0055] ①Double-enzyme synchronous enzymatic hydrolysis

[0056] Mix sesame meal and distilled water according to the material-to-liquid ratio of 1:20 (g:mL), adjust the pH to 10.0 with 1 mol / L sodium hydroxide solution, and use the enzyme activity unit of alkaline protease and papain at 2:1 (U: U), add alkaline protease and papain (food grade), add 8000 U per gram of sesame meal, 55°C dual-enzyme synchronous enzymatic hydrolysis for 4 h, and 95°C for 15 min to obtain enzymatic hydrolyzate;

[0057] The sesame meal is a by-product of preparing sesame oil by the water substitution method, with a water content of 67.3% and a protein content of 12.6%;

[0058] The simultaneous enzymatic hydrolysis of the dual enzymes is the direct hydrolysis of sesame meal by alkaline protease and papain;

[0059] The enzyme inactivation is to inactivate alkaline protease and papain at 95°C and 15 min;

[0060] ②Separation and purification of enzymatic hydrolyzate

[0061] Adjust the pH of the ...

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Abstract

The invention discloses a method for preparing sesame oligopeptide from a water extraction method sesame oil byproduct, and belongs to the technical field of biology. According to the method, the byproduct sesame meal of sesame oil prepared by a water extraction method is used as a raw material, synchronous enzymolysis is performed by adopting double enzymes, separation and purification are performed through activated carbon decoloration, microfiltration and ultrafiltration, and spray drying is performed to obtain a sesame oligopeptide product, the hydrolysis degree is 33.9%, the peptide fragment of the oligopeptide product less than 1000 Da accounts for 87.5%, and the purity reaches 91.4%. The sesame oligopeptide preparation method has the advantages of high hydrolysis degree, short time consumption, high product purity, faint yellow color, good application value, and suitableness for large-scale production.

Description

technical field [0001] The invention relates to a method for preparing sesame oligopeptides from by-products of sesame oil by a water substitution method, and belongs to the field of biotechnology. Background technique [0002] Sesame is one of the four major oil crops in my country, containing 45%-63% oil, 20%-27% protein, and 50% of sesame seeds are used for oil production. The water substitution method is a traditional method of producing sesame oil in my country, which accounts for more than 40% of the annual processing volume of sesame oil, and the by-product produced in the production process is sesame meal. Every ton of sesame oil produces 1 ton of sesame meal, and the country produces about 160,000 tons of sesame meal every year. Sesame meal contains about 40% protein and 2.3-11.0% crude fat (based on dry weight of sesame meal), and is rich in protein resources. The content of glutamic acid and aspartic acid in sesame protein is the highest, and the content of cyst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/22
CPCC12P21/06C07K1/36C07K1/34C07K1/22
Inventor 王元秀李孟畛丁良徐新燕李新宇程文李晨赵晓畅
Owner UNIV OF JINAN
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