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Preparation method of globe artichoke extract

An artichoke extract and artichoke technology, applied in the field of preparation of artichoke extract, can solve the problems of unfriendly environment, limited application range, and low content of active ingredients, so as to reduce production cost, improve adsorption effect, and shorten production the effect of time

Active Publication Date: 2022-05-06
CHANGSHA LANGLIN BIOLOGY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method heat reflux extraction, does not protect the heat-sensitive caffeoylquinic acid substances, the yield is low, and secondly, n-butanol is used as a toxic and flammable solvent, which is not environmentally friendly
At present, the content of artichoke leaf extract cymarin in the international market is mainly concentrated in 2.5% to 10%, and the content of artichoke leaf extract chlorogenic acid is mainly concentrated in 1.2% to 2.5%. The content of its main active ingredients is low. And there is a risk of excessive pesticide residues, which limits the scope of application, and the demand for high content and low pesticide residues of artichoke extract is increasing day by day

Method used

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  • Preparation method of globe artichoke extract
  • Preparation method of globe artichoke extract
  • Preparation method of globe artichoke extract

Examples

Experimental program
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Effect test

preparation example Construction

[0107] 1. Preparation of reference solution:

[0108] Accurately weigh 2.85 mg of chlorogenic acid reference substance and place in a 25 mL volumetric flask, dilute to volume with methanol, shake well, and prepare chlorogenic acid solution.

[0109] 2. Preparation of the test solution:

[0110] Accurately weigh an appropriate amount of extract, add a volume fraction of 50% methanol aqueous solution into a 25mL volumetric flask, dissolve it ultrasonically, place it at room temperature, add dropwise 50% methanol aqueous solution to 25mL and shake well, dilute accordingly according to the absorbance, and use it as the extract The test solution.

[0111] 3. Selection of measurement wavelength:

[0112] Scan the reference substance solution and the test solution at 200nm to 400nm, both of which have maximum absorption at 327nm wavelength, so the detection wavelength is selected as 327nm.

[0113] 4. Drawing and linear range of standard curve:

[0114] Precisely draw 0.2mL, 1mL,...

Embodiment 1

[0125] This embodiment is a preparation method of artichoke extract, comprising the following steps:

[0126] S1. Dual-frequency ultrasonic extraction:

[0127] Artichoke raw material 500g, be pulverized to 40 order, add 12 times of volume fraction and be 60% acidic ethanol aqueous solution (pH is 3, adopt citric acid to regulate pH; Also contain VC in this solvent, the addition amount of VC is relative to the quality of artichoke raw material 0.2%); low-temperature extraction assisted by dual-frequency ultrasonic waves, solid-liquid separation after extraction, and extraction liquid collected.

[0128] Among them, the parameters of dual-frequency ultrasonic-assisted cryogenic extraction are as follows:

[0129] Low-frequency 15kHz, high-frequency 30kHz (low-frequency and high-frequency alternately, each time 30s; that is, low-frequency extraction for 30s, then switching to high-frequency extraction for 30s), 50°C extraction once, extraction time 60min.

[0130] S2. Concentr...

Embodiment 2

[0145] This embodiment is a preparation method of artichoke extract, comprising the following steps:

[0146] S1. Dual-frequency ultrasonic extraction:

[0147] Artichoke raw material 500g, be pulverized to 40 order, add 15 times of volume fraction and be 80% acidic ethanol aqueous solution (pH is 4, adopt citric acid to regulate pH; Also contain VC in this solvent, the addition amount of VC is relative to the quality of artichoke raw material 0.4%); low-temperature extraction assisted by dual-frequency ultrasonic waves, solid-liquid separation after extraction, and extraction liquid collected.

[0148] Among them, the parameters of dual-frequency ultrasonic-assisted cryogenic extraction are as follows:

[0149] Low frequency 29kHz, high frequency 40kHz (low frequency and high frequency alternately, each time 30s), 30 ℃ extraction twice, single extraction time 30min.

[0150] S2. Concentration under reduced pressure:

[0151] The extract prepared in step S1 was concentrated...

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Abstract

The invention discloses a preparation method of a globe artichoke extract, and the preparation method comprises the following steps: S1, carrying out double-frequency ultrasonic extraction on a globe artichoke raw material to prepare an extracting solution; s2, adding an adsorbent into the extracting solution prepared in the step S1 for reaction, carrying out solid-liquid separation, and collecting a liquid phase; s3, the liquid phase prepared in the step S2 is eluted through a polyamide chromatographic column, eluents are water and an ethanol water solution in sequence, and eluent is collected; wherein an extracting agent for double-frequency ultrasonic extraction in the step S1 is an acidic ethanol solution; the adsorbent in the step S2 is chitosan modified diatomite-activated carbon. The preparation process is simple and safe, the obtained globe artichoke extract is natural, healthy, pure in flavor, high in yield and low in preparation cost, and industrial production can be achieved.

Description

technical field [0001] The invention relates to the technical field of plant processing and extraction, in particular to a preparation method of artichoke extract. Background technique [0002] Artichoke (Cynara scolymus L.) is a large perennial herb of the genus Artichoke in the Comopsite family. It is also known as artichoke, ball thistle, artichoke and French lily. But for a long time, people only eat its flower buds, and a large number of stems and leaves are discarded. [0003] Artichoke leaves contain a variety of phenolic compounds, namely caffeoylquinic acid derivatives, hydroxycinnamic acid derivatives, quercetin derivatives, coumarin derivatives, luteolin derivatives and apigenin derivatives, etc. , the total polyphenols accounted for 3.27% to 4.23% of the dry matter mass fraction, and caffeoylquinic acid compounds were the most important components in the total polyphenols, accounting for about 60% to 90% of the total polyphenols, while chlorogenic acid The mass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/732
CPCC07C67/48C07C67/56C07C2601/14C07C69/732
Inventor 贺玉婷
Owner CHANGSHA LANGLIN BIOLOGY PROD
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