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Chitosan-based biological tissue adhesive and its preparation method and application

A biological tissue and adhesive technology, used in applications, surgical adhesives, drug delivery, etc., can solve the problems of product cytotoxicity and weak adhesion, and achieve strong wet tissue adhesion, good mechanical properties, The effect of excellent hemostatic efficiency

Active Publication Date: 2022-07-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many limitations in the clinical application of tissue adhesives, such as weak adhesion with wet surface tissue, not easy to degrade or degradation products have potential cytotoxicity, and are likely to induce persistent tissue inflammatory reactivity and other risks

Method used

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  • Chitosan-based biological tissue adhesive and its preparation method and application
  • Chitosan-based biological tissue adhesive and its preparation method and application
  • Chitosan-based biological tissue adhesive and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of TEMPO oxidized chitosan

[0040] Under light-proof conditions, accurately weigh 0.2 g of TEMPO, dissolve it in deionized water, and continue stirring at 200 rpm until the dissolution is uniform. Then 200 mL of sodium hypochlorite solution (available chlorine concentration 4%), the main oxidant, was slowly added, and the pH was adjusted to 7.8 using 1 M hydrochloric acid.

[0041] 12 g of powdered chitosan was added to the above TEMPO oxidation system, and the reaction was continued and slowly stirred for 2 hours at room temperature. During the reaction, 0.5M NaOH solution was used to maintain the pH value of the reaction system stable. Anhydrous ethanol (1:10, v / v) was added to stop the oxidation reaction, and the pH was adjusted to 7.0, and then the oxidation product was precipitated after standing for 30 min. The pellets were washed 6 times by repeated centrifugation using graded ethanol solution to remove unreacted reagents or impurities. T...

Embodiment 2

[0044] Example 2 Preparation of biological tissue adhesive

[0045]Disperse 2 g of TEMPO oxidized chitosan and 30 g of organic polymer monomer acrylic acid prepared in Example 1 in 100 ml of deionized water, continue stirring at room temperature at 300 rpm until a homogeneous solution is obtained, and then add 2.0 mL of 0.35M The free radical initiator ammonium persulfate solution, the hydrogel prepolymerization solution was injected into a closed glass reaction mold with a thickness of 100 μm using a disposable sterile syringe, and the hydrogel was prepared overnight at 60 °C through a free radical initiating polymerization reaction. glue. After the hydrogel was formed, the hydrogel was immersed in a 75% ethanol solution to remove unreacted impurities, and then it was completely dried. Finally, the tissue adhesive patch and desiccant were placed in a sealed bag and stored at -20°C for later use.

[0046] Using a microcomputer-controlled electronic universal testing machine ...

Embodiment 3

[0047] Example 3 Application of biological tissue adhesive

[0048] Using an isolated porcine organ model, the adhesive hemostatic properties of tissue adhesives under wet and dynamic conditions were evaluated. In vitro adhesion hemostasis experiments were performed using fresh porcine hearts as models. A hole with a diameter of 10-20 mm was punctured in the pig heart, and then a perfusion tube with a liquid flow rate of 20 mL / min was inserted into the pig heart to simulate massive hemorrhage. Use tissue adhesive (prepared in Example 2) to attach to the damaged organ and seal the incision after pressing for 5 s, and monitor the liquid leakage and sealing of the tissue adhesive.

[0049] The experimental results are as image 3 As shown, the tissue adhesive can be directly applied to the damaged part of the organ, and after being pressed with mild pressure (about 1 kPa) for 5 s, it can achieve tight adhesion and form a liquid seal, and no leakage of liquid at the damaged part...

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Abstract

The invention discloses a chitosan-based biological tissue adhesive, which is prepared by the following methods: (1) dissolving organic macromolecular monomers and oxidized chitosan in water, and mixing to obtain a precursor solution; the The organic polymer monomer is selected from any one or more of acrylamide, acrylic acid, ethylene glycol or vinyl alcohol; the oxidized chitosan refers to the oxidation of the primary alcohol group on the polysaccharide molecular chain to an aldehyde carbonyl group. Chitosan; (2) adding a free radical initiator to the precursor solution and mixing to obtain a prepolymerized solution; (3) passing the prepolymerized solution through a film and injecting it into a closed glass reaction mold to initiate polymerization under heating conditions, Obtain biological tissue adhesive. The biological tissue adhesive of the present invention has strong wet surface tissue adhesion and excellent hemostatic ability, and can be used to prepare products with the function of promoting hemostasis and closure of surgical or tissue wounds and / or the function of promoting wound healing. Prospects are good.

Description

technical field [0001] The invention relates to a chitosan-based biological tissue adhesive, a preparation method and application thereof, and belongs to the technical field of biomedical materials. Background technique [0002] With the development of materials science and medical technology, tissue adhesives provide a more convenient and efficient means for surgical suturing of wounds, which can quickly close wounds under moist and dynamic conditions, achieve hemostasis and prevent leakage. In clinical applications, the function of tissue adhesives is currently mainly to replace or assist traditional surgical sutures and staples for wound closure, which can improve surgical efficiency to a certain extent. Tissue adhesive has good mechanical flexibility and tensile properties, which can effectively avoid re-trauma of new granulation tissue, and can provide a moist and mild environment for wounds, effectively avoid wound infection and promote wound healing. The application ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08A61L24/00C08F251/00C08F220/06
CPCA61L24/08A61L24/001C08F251/00A61L2400/04C08F220/06C08L5/08
Inventor 毛相朝黄文灿何永均
Owner OCEAN UNIV OF CHINA
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