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Preparation process of bacteriostatic bacillus subtilis microecological preparation

A technology of Bacillus subtilis and microecological preparations, which is applied in the field of preparation technology of bacteriostatic Bacillus subtilis and microecological preparations, can solve problems such as the gap in effect and achieve the effect of strong high temperature resistance and strong antibacterial properties

Pending Publication Date: 2022-05-13
浙江启润生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, people have researched and developed antibiotic substitutes such as feed enzyme preparations, feed microbial preparations, acidifiers, antibacterial peptides, oligosaccharides, allicin, Chinese herbal medicines, and organometallic trace elements. These green feed additives have been favored by people. Favored, are all based on the research on the basis of inhibiting the proliferation of harmful bacteria, promoting the proliferation of beneficial bacteria, and improving the intestinal microenvironment, but generally speaking, compared with antibiotics, these antibiotic substitute products still have a certain gap in their effect

Method used

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  • Preparation process of bacteriostatic bacillus subtilis microecological preparation
  • Preparation process of bacteriostatic bacillus subtilis microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The separation and purification of embodiment 1 Bacillus subtilis QR-KC-008:

[0034] (1) Sample processing:

[0035] The sandy soil near the company was selected as the sample, and the soil sample was boiled in sterile water at 90°C for 20 minutes.

[0036] (2) Then use an inoculation loop to dip the treated sample solution into the solidified plate culture medium on the slope of the mother species, mark the line, and incubate in a 40°C incubator at a constant temperature for 24 hours, and pick colonies with good growth and similar colonies And the colony with the largest number is isolated and purified until the single bacteria with the same colony shape, and the isolated single bacteria are subjected to protease detection test, bacteriostatic test, and Gram staining microscope inspection.

[0037] The plate medium is: 2% glucose, 0.5% peptone, 0.1% yeast extract, 0.02% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 1% sodium chloride, 2% agar, pH 7.0.

[...

Embodiment 2

[0054] Example 2 Bacillus subtilis QR-KC-008 fermentation production process.

[0055] The preferred bacterial strain QR-KC-008 obtained by screening in Example 1 was expanded and cultivated to prepare bacterial powder. The specific production process is as follows:

[0056] The preparation process of Bacillus subtilis bacterial agent of the present invention comprises the following concrete steps:

[0057] (1) The strain is Bacillus subtilis strain numbered QR-KC-008;

[0058] (2) Cultivation of slant mother species: Inoculate Bacillus subtilis strains preserved in glycerol freeze-dried tubes on solid slant medium and culture at 40°C for 2 days;

[0059] (3) Erlenmeyer flask mother seed culture: Inoculate the prepared slant mother seed under sterile conditions, pick two rings with an inoculation loop and put them in 150mL triangular flask mother seed medium, and cultivate at 40°C, 150r / min for 1.5 d, making the first-grade triangular flask seed solution;

[0060] (4) Expan...

Embodiment 3

[0067] Example 3 Preparation process of Bacillus subtilis QR-KC-008 probiotics.

[0068] Use the QR-KC-008 fermented in Example 2 to ferment, use the GQ-105 centrifuge to set the speed at 16000r / min, feed the flow rate at 2.68L / min, and centrifuge. After the centrifugation, collect the sludge and place it in a 4°C freezer Save for later use. Centrifuge the bacteria slurry to dissolve.

[0069] The preparation process of Bacillus subtilis QR-KC-008 probiotics mainly includes the following steps:

[0070] (1) Preparation of Bacillus subtilis QR-KC-008 suspension, using sterile saline at 250r / min to stir for 15min to prepare the centrifuged sludge into 30% (w / v) bacterial suspension;

[0071] (2) Preparation of core material mixture mixture: Sodium alginate 2% (w / v), glycerin 4.5% (w / v), carbogel 0.5% (w / v), Tween 80 0.2% (w / v) Add 30% (w / v) of Bacillus subtilis QR-KC-008 to a certain amount of distilled water and mix well;

[0072] (3) Inject the core material mixture prepa...

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Abstract

The invention discloses a preparation process of a bacteriostatic bacillus subtilis microecological preparation. The bacteriostatic bacillus subtilis microecological preparation is named as bacillus subtilis QR-KC-008. The bacillus subtilis QR-KC-008 provided by the invention has a relatively strong inhibition effect on staphylococcus aureus, escherichia coli and fusarium, meanwhile, the activity of enzyme produced by fermentation metabolism is relatively high, and based on the specific advantages, the bacillus subtilis microecological preparation provided by the invention can be applied to the field of replacing antibiotics in feed additives.

Description

technical field [0001] The invention belongs to the technical field of microecological feed additives, and in particular relates to a preparation process of a bacteriostatic Bacillus subtilis and a microecological preparation. Background technique [0002] Due to the abuse of various antibiotics, hormones and illegal drugs to feed livestock and poultry, frequent food safety incidents and the emergence of drug-resistant strains have brought about huge potential food safety risks. It can be seen that the development of high-efficiency and high-quality biological feed products to replace antibiotics, Hormones, the production of green pollution-free, organic meat, eggs, and milk is a livelihood issue required by the country and the food safety of the whole people. [0003] In recent years, people have researched and developed antibiotic substitutes such as feed enzyme preparations, feed microbial preparations, acidifiers, antibacterial peptides, oligosaccharides, allicin, Chines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10A23K10/18C12R1/125
CPCC12N1/20C12N11/10A23K10/18
Inventor 孙标陈维菁朱煜冬张富明韩立虎
Owner 浙江启润生物科技有限公司
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