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Quantum dot fluorescence coded polylactic acid microspheres as well as preparation method and application thereof

A technology of polylactic acid microspheres and fluorescent encoding, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, can solve the problems of few applications of polylactic acid microspheres, difficulty in rapid preparation, and poor activity of microspheres, and achieve good results. Biocompatibility, rapid preparation, and low-cost effects

Pending Publication Date: 2022-05-17
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current process of using polylactic acid to prepare microspheres has many shortcomings, such as poor stability, poor activity of microspheres, difficulty in rapid preparation, and few applications of polylactic acid microspheres.

Method used

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  • Quantum dot fluorescence coded polylactic acid microspheres as well as preparation method and application thereof
  • Quantum dot fluorescence coded polylactic acid microspheres as well as preparation method and application thereof
  • Quantum dot fluorescence coded polylactic acid microspheres as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1.1. Preparation of polylactic acid polymer (PLA-MA)

[0049] Dissolve 5g of polylactic acid (Mw=110000), 0.5g of maleic anhydride, and 0.01g of benzoyl peroxide in 50mL of dichloromethane, then place the solution in a vacuum oven and dry at room temperature for 24 hours to achieve the Fully fused together; finally, the dried white solid mixture was reacted in an oven at 110°C for 18 hours, and a light yellow solid mixture was obtained after the reaction; purified by precipitation with chloroform lysate and diethyl ether for many times, and centrifuged at 10,000rpm 3 minutes, repeated 3 times; finally, the solid mixture was vacuum-dried at 40°C for 10 hours, and stored at room temperature for future use, laying the foundation for the subsequent preparation of polymer fluorescent microspheres.

[0050] 1.2. Polylactic acid polymer performance test

[0051] The prepared polymer is subjected to zeta potential test, suspended in 5% SDS solution, the test result is shown in...

Embodiment 2

[0053] Preparation of polylactic acid fluorescent microspheres and its carboxylation, such as image 3 shown.

[0054] Polylactic acid-maleic anhydride fluorescent microspheres are prepared by SPG membrane emulsification method combined with solvent evaporation method. Such as image 3 As shown, pour 4mL of the chloroform solution containing 6.25% of the polylactic acid polymer prepared in Example 1 and 10mg of oily ZnS / CdSe QDs (wavelength is 525nm) into the liquid storage tank, and when the pressure is stabilized at 0.008MPa, Open the valve connected to the liquid storage tank, and the dispersed phase in the liquid storage tank will pass through the uniform micropores on the SPG membrane under the action of 0.008MPa to form droplets on the surface of the other membrane, and the droplets will be quickly contained by the continuous phase in the beaker. Active agent SDS package. Wherein the continuous phase is an aqueous solution containing 0.5wt% SDS. Through the continuou...

Embodiment 3

[0056] This embodiment evaluates the fluorescence stability of polylactic acid-maleic anhydride fluorescent microspheres as follows: Figure 5 As shown, the steps are as follows:

[0057] The polylactic acid-maleic anhydride fluorescent microspheres prepared in Example 2 were placed at different temperatures for 24 hours, by Figure 5 As shown in (a), the signal of the microspheres is basically not affected after being placed in the range of 4-50°C for 24 hours, but the fluorescence performance of the microspheres is reduced by 20%-40% after being treated at 70°C and 90°C for 24 hours, which shows that the PLA-MA quantum Dot fluorescent microspheres have good temperature stability and can basically meet the temperature conditions required as an immunoassay carrier;

[0058] The polylactic acid-maleic anhydride fluorescent microspheres that embodiment 2 is obtained is dispersed in the solvent of different pH (pH is respectively 1,3,5,7,9,11,13), measures its fluorescence inten...

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Abstract

The invention discloses quantum dot fluorescence coded polylactic acid microspheres as well as a preparation method and application thereof, a liquid-phase suspension biochip system is constructed based on the quantum dot fluorescence coded polylactic acid microspheres, biological activity is rapidly judged through test strip immunoassay, and finally the quantum dot fluorescence coded polylactic acid microspheres are applied to immunodetection of tumor markers. According to the system, a novel polylactic acid fluorescent microsphere is used as a carrier, and water-phase CdTe QDs is used as a signal substance. The immunodetection system comprises a novel polylactic acid fluorescent microsphere-coated tumor marker capture antibody, a water-phase CdTe QDs-labeled tumor marker antibody, and a tumor marker antigen which is respectively connected with the novel polylactic acid fluorescent microsphere-coated tumor marker capture antibody and the water-phase CdTe QDs-labeled tumor marker antibody through antigen-antibody interaction. The method has the characteristics of quick reaction, good repeatability, simplicity and convenience in operation, high throughput, multi-index combined detection and high sensitivity in tumor marker detection.

Description

technical field [0001] The invention relates to the field of immunodetection, in particular to a quantum dot fluorescence-encoded polylactic acid microsphere, its preparation method and application, and its application in the immunodetection of tumor markers. Background technique [0002] The liquid-phase suspension biochip technology uses suspended microspheres as the carrier of the liquid phase reaction, and uses fast and high-throughput flow cytometry as the analysis method for rapid and high-throughput multivariate detection. Polymer fluorescence-encoded microspheres are the core of liquid-phase suspension biochip technology, and the selection of polymer directly determines whether the constructed bio-suspension chip system can have efficient detection performance. At present, most of the polymers used are polystyrene, polystyrene-maleic anhydride and other difficult-to-degrade polymers. Although it can be effectively carried out in immune detection, it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02C08F283/02C08F222/06C08J3/12C08L51/08G01N21/64G01N33/533G01N33/543G01N33/558G01N33/574
CPCC09K11/883C09K11/02C09K11/025C08F283/02C08J3/12G01N21/6428G01N33/533G01N33/54313G01N33/558G01N33/57473G01N33/57484C08J2351/08G01N2333/47C08F222/06
Inventor 丁收年汤万升张博
Owner SOUTHEAST UNIV
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