Unlock instant, AI-driven research and patent intelligence for your innovation.

High-brightness near-infrared fluorescent composite microsphere and preparation method thereof

A composite microsphere and near-infrared technology, applied in the field of fluorescent labeling, can solve the problems of low structural stability, poor uniformity of microspheres, weak fluorescence intensity, etc., and achieve the effects of improving labeling efficiency, less background interference, and low detection limit

Active Publication Date: 2022-05-20
CHINA PHARM UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the quantum dot fluorescent microspheres prepared by the silica coating method have certain stability, the coating amount of a single microsphere is small, resulting in weak fluorescence intensity; the poor uniformity of the small-sized silica microspheres limits its application
Compared with other traditional surface modification technologies, the biggest advantage of LbL is its controllability. By controlling the number of adsorbed molecular layers, the physical properties of the multilayer film, such as mechanical properties, can be adjusted to the nanoscale. precision; and the structure and chemical properties of the assembled multilayer film can be regulated by changing the polyelectrolyte or other charged species; but the existing disclosed fluorescent microspheres based on layer-by-layer self-assembly technology still have low fluorescence intensity and Problems of short lifetime and low structural stability due to relying only on weak forces such as electrostatic forces and hydrogen bonds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-brightness near-infrared fluorescent composite microsphere and preparation method thereof
  • High-brightness near-infrared fluorescent composite microsphere and preparation method thereof
  • High-brightness near-infrared fluorescent composite microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A kind of preferred concrete preparation process of embodiment 1 fluorescent composite microsphere of the present invention

[0047] The synthetic process of indocyanine green dye Cypate used below is as references: Ye, Y.; Bloch, S.; Kao, J.; Achilefu, S., Multivalent Carbocyanine Molecular Probes: Synthesis and Applications.Bioconjugate Chemistry 2005,16 ), 51-61. The steps are recorded in, and its UV and fluorescence properties are as follows Figure 4 shown;

[0048] Synthesis of dye silica precursor: weigh 4mg (0.0064mmol) of Cypate dye and dissolve it in 1mL DMF, add 2.5mg (0.013mmol) of EDC·HCl and 1.5mg (0.013mmol) of NHS, and react in the dark for 3 to 6 hours , and then add 1.8 μL or 0.0064 mmol of APTES to continue the reaction for 12 hours to obtain the dye silica precursor solution Cypate-APTES;

[0049] Synthesis of dye silica microspheres: Take 50mL absolute ethanol, add 4mL5 ammonia water and 1mL water, stir for 30min, add 250μL of the above-mentioned ...

Embodiment 2

[0055] Embodiment 2 changes the feeding amount of ammonia water to control microsphere size

[0056]Set up 4 comparison groups, each group in the synthesis step of dye silica microspheres, set different ammonia water feeding amount, the added ammonia water (25%~28%wt.) dosage is respectively 2mL (concentration 0.5M), 3mL (0.7M) and 4mL (0.9M), the rest of the operations were obtained under the same conditions as in Example 1 to obtain different sizes of dye silica microspheres Cypate@SiO 2 powder;

[0057] Such as Figure 8 Shown is the size control diagram of the dye silica microspheres obtained under different ammonia feeding conditions.

Embodiment 3

[0058] Embodiment 3 changes the synthesis time to achieve the adjustable emission of quantum dots

[0059] Set up 5 comparison groups, each group is in the synthesis step of CdTe / CdS quantum dots, the specific operation is as follows: Weigh 90mg of NaBH 4 (about 2mmol) dissolved in 2mL of distilled water, quickly degassed under nitrogen for 0.5h, the whole process was carried out under ice bath, quickly added 127mg (1mmol) of tellurium powder, continued to react for 0.5h, sealed the ep tube and placed it in 4 ℃ refrigerator, the solution slowly turns into a transparent purple solution, which is NaHTe solution. Take 366.6mg (2mmol) CdCl 2 Dissolve in 100mL of distilled water, add 314μL (3.6mmol) of 3-mercaptopropionic acid, add 2M NaOH solution dropwise, adjust the pH to alkaline, then quickly add the prepared anaerobic NaHTe into the above solution, stir well . Finally, transfer 10mL of the mixed precursor solution to a stainless steel autoclave lined with polytetrafluoroet...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a high-brightness near-infrared fluorescent composite microsphere and a preparation method thereof, and the preparation method comprises the following steps: doping Cypate dye into silicon dioxide nanoparticles by using a method to obtain dye silicon dioxide microsphere Cypate coated SiO2 microspheres; the preparation method comprises the following steps: carrying out amination on the surface of a microsphere, carrying out layer-by-layer self-assembly of quantum dots on the surface of the aminated dye microsphere by using PAH, carrying out carboxylation on the surface of the assembled microsphere by using polyacrylic acid (PAA), and adding glutaraldehyde to increase chemical connection of two adjacent PAH layers so as to obtain the high-brightness near-infrared fluorescent composite microsphere.

Description

technical field [0001] The invention relates to the technical field of fluorescent labeling, in particular to a high-brightness near-infrared fluorescent composite microsphere and a preparation method thereof. Background technique [0002] Antigen-antibody labeling reagents are used in the immunochromatography process, and rapid diagnosis of diseases can be achieved through specific recognition and binding of disease markers. However, in the prior art, such as colloidal gold and dye / quantum dot microspheres commonly used as antigen antibody labeling reagents in immunochromatography, there are technical problems of low sensitivity, high interference in blood samples, that is, low relative fluorescence intensity. [0003] As a new type of fluorescent labeling material, quantum dots have the excellent properties of broad excitation, narrow emission peak and resistance to photobleaching. However, the shortcomings of quantum dots such as small particle size and high surface ener...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/88B82Y30/00B82Y40/00B82Y20/00
CPCC09K11/06C09K11/883B82Y30/00B82Y40/00B82Y20/00C09K2211/1029
Inventor 邓大伟欧阳学良朱梦仟刘学成蒋丽文赵晓敏
Owner CHINA PHARM UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com