Application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora

A technology for regulating intestinal flora and maltopentaose, applied in the biological field, can solve the problem that maltopentacosyl trehalose cannot reach the colon completely, and it is impossible to study the role of maltopentacosyl trehalose microspheres in regulating intestinal flora It can inhibit the growth of harmful bacteria, help nutrition and absorption, and increase immunity.

Active Publication Date: 2022-05-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the α-1,4 glycosidic bonds will be hydrolyzed by α-amylase and glucoamylase, maltopentaosyl trehalose cannot reach the colon completely, so it is impossible to study the role of maltopentaosyl trehalose microspheres in regulating intestinal The role of the flora

Method used

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  • Application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora
  • Application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora
  • Application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of preparation method of maltopentacosyl trehalose microspheres

[0025] (1) Preparation of maltopentaosyl trehalose-sodium alginate mixed solution: dispersing sodium alginate in deionized water, stirring and dissolving at 60° C. and cooling to room temperature to obtain a sodium alginate solution, and uniformly dispersing maltopentaosyl trehalose Disperse in sodium alginate solution, and after uniform dispersion, ultrasonically eliminate bubbles to obtain maltopentacosyl trehalose-sodium alginate mixed solution. The final mass fractions of sodium alginate in the mixture were 1%, 2%, and 3%, respectively, and the mass ratio of sodium alginate to maltopentacosyl trehalose was 2:9.

[0026] (2) Preparation of maltopentacosyl trehalose microspheres: the above mixed solution is pumped into CaCl with a mass fraction of 4% through a peristaltic pump 2 solution, microspheres in CaCl 2 Store in a bath for 3h. The microspheres were collected by filtration...

Embodiment 2

[0033] The digestion process of the maltopentaosyl trehalose microspheres (2% sodium alginate concentration) obtained in Example 1 in the upper alimentary tract such as the oral cavity, the stomach and the small intestine. The sample solutions involved in this example include: (I) a 20 mg / mL maltopentacosyl trehalose solution, (II) a microsphere solution with a solute concentration equivalent to the unembedded maltopentacosyl trehalose solution, The preparation method of the microsphere solution is to use 100 mg of maltopentacosyl trehalose to make microspheres, and then add water to directly prepare a 5mL solution. At this time, the concentration of maltopentacosyl trehalose in the microsphere solution is equivalent to 20mg / ml.

[0034] (1) Simulated oral digestion: sample solutions with a concentration of 20 mg / mL were prepared respectively. Set up A, B, C experimental groups. Group A was added with 1 mL of saliva and 5 mL of sample solution, group B was added with 1 mL of ...

Embodiment 3

[0041] Simulated colonic fermentation of maltopentacosyl trehalose and microspheres (sodium alginate concentration 2%) obtained in Example 1. The changes of each index (pH, bacterial content, gas production and short-chain fatty acids) and the adjustment of intestinal flora structure were detected.

[0042] (1) Preparation of intestinal flora seed solution: Three volunteers (2 males and 1 female, aged 20- between 30 years of age), collect fresh feces and seal them for disposal. After mixing with the ratio of 1 g mixed feces: 10 mL phosphate buffer (0.1 M, pH 7.0), it was filtered through 4 layers of gauze, and the filtrate was the intestinal flora seed liquid, which was used for later use.

[0043] (2) Simulated in vitro fermentation: The seed solution was placed in a non-carbon source at an inoculum of 10%, containing 2g / L peptone, 2g / L yeast extract, 0.1g / L NaCl, 0.04g / L KH 2 PO 4 , 0.04g / L K 2 HPO 4 , 0.01g / L CaCl 2 ·2H 2 O, 0.01g / LMgSO 4 ·2H 2 O, 2g / L NaHCO 3 , 0...

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Abstract

The invention discloses application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora, and belongs to the technical field of biology. The preparation method of the maltopentasosyl trehalose microspheres, disclosed by the invention, comprises the following steps: (1) preparing a maltopentasosyl trehalose-sodium alginate mixed solution; and (2) preparing the maltopentaglycosyl trehalose microspheres. The maltopentaglycosyl trehalose microspheres are almost not digested when passing through the upper digestive tracts such as the oral cavity, the stomach, the small intestine and the like, and the maltopentaglycosyl trehalose is protected to completely reach the colon part. According to the microspheres disclosed by the invention, the abundance of pathogenic bacteria is reduced by remarkably improving the abundance of probiotics in an in-vitro fermentation process, so that the intestinal flora of a human body is regulated. Meanwhile, the ratio of the firmicobacteria to the bacteroides can be promoted to be reduced, the host capacity absorption is reduced, and the composition is beneficial to obese people.

Description

technical field [0001] The invention relates to the application of maltopentacosyl trehalose and its microspheres in regulating intestinal flora, and belongs to the field of biotechnology. Background technique [0002] The large number of intestinal flora affects the digestion and absorption of food and energy supply. The composition of human intestinal flora is related to many diseases, such as obesity, diabetes, allergies, etc. Therefore, the regulation of gut microbes has attracted more and more attention. In recent years, the prebiotic effects of various novel oligosaccharides and polysaccharides have been demonstrated. Studies have shown that undigested sugars in the upper gastrointestinal tract can enter the large intestine and be digested and utilized by intestinal flora to produce short-chain fatty acids, promote the growth of beneficial microorganisms, and have anti-inflammatory, hypoglycemic, and anti-cancer effects. Some common carbohydrates, such as fructooligo...

Claims

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Application Information

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IPC IPC(8): A23L33/125A23P10/30B01J13/02A61K31/715A61P3/04A61P3/10A61P3/06
CPCA23L33/125A23P10/30B01J13/02A61K31/715A61P3/04A61P3/10A61P3/06A23V2002/00A23V2200/32A23V2200/332A23V2200/3262A23V2200/328Y02A50/30
Inventor 江波潘莹陈静静
Owner JIANGNAN UNIV
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