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Nano artificial antibody with broad-spectrum antiviral effect and preparation method thereof

An artificial antibody and virus technology, applied in medical preparations containing active ingredients, antiviral agents, nanotechnology, etc., can solve the problems of poor stability, expensive antibodies and lectins, and less glycans, and achieve the effect of inhibiting infection Effect

Pending Publication Date: 2022-05-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the weak immunogenicity of sugars and the difficulty of preparation, there are few reagents that can specifically recognize glycans
Currently reported specific affinity reagents capable of binding high mannose are limited to a few antibodies and lectins
However, both antibodies and lectins have the problems of high price and poor stability, and their inhibitory effect on viruses is unknown
In addition, most of the existing reagents that can recognize homomannan are monovalent or bivalent, and the reagents that can multivalently bind to homomannan have not been reported yet.

Method used

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  • Nano artificial antibody with broad-spectrum antiviral effect and preparation method thereof
  • Nano artificial antibody with broad-spectrum antiviral effect and preparation method thereof
  • Nano artificial antibody with broad-spectrum antiviral effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Nano-artificial antibody Preparation of nano-artificial antibody

[0059] 1) The preparation method of the nano-artificial antibody of the present invention comprises the steps as follows:

[0060] Step 1: Preparation of functional monomer, weigh 1.86g 3-methyl-2,4-difluorophenylboronic acid, 4g 4A molecular sieve (Item No.: M24119-100G; brand: MERYER), 1.26g sodium cyanoborohydride, add 80 mL of anhydrous methanol was stirred to dissolve, 5 mL of 3-aminopropyltriethoxysilane was pipetted, and the reaction was carried out at room temperature for 24 h. After the reaction, filter, spin dry solvent methanol, wash with petroleum ether and ethyl acetate, and vacuum dry to obtain functional monomer.

[0061] Step 2: Weigh 100 mg of benzyl-modified mannose as template, 1.77 g of Triton-100, pipette 7.5 mL of cyclohexane, 1.6 mL of n-hexanol, stir magnetically for 10 min, add 480 μL of water, 100 μL of concentrated ammonia water, and continue stirring After 12 hours...

Embodiment 2

[0072] Example 2 Determination of the binding site of nano-artificial antibody to mannose

[0073] 1) Experimental procedure: A series of standard solutions of benzyl-modified mannose were prepared with phosphate buffer (10 mM, pH 7.4). Disperse 2 mg of the nanoartificial antibody prepared in Example 1 into 200 μL of the standard solution. After shaking the dispersion on a rotator for 2 hours at room temperature, the nanoartificial antibody was collected by centrifugation and washed 3 times with 200 μL of phosphate buffer (10 mM, pH 7.4). Elute with 20 μL of 100 mM acetic acid solution and collect the supernatant by centrifugation. The content of adsorbed benzylmannose was determined by measuring the UV absorbance of the supernatant at 230 nm. The adsorption isotherm was determined by plotting the UV absorbance at 230 nm of the supernatant versus the logarithmic concentration of benzylmannose. In order to estimate the binding affinity of the nanoartificial antibody to manno...

Embodiment 3

[0079] Example 3 Nano-artificial antibody specific binding to mannose experiment

[0080] Using the obtained nano-artificial antibody prepared by the method of Example 1, the nano-artificial antibody with more than 2000 binding numbers to mannose was selected as the material group (named as MIP)

[0081] Set the template-free molecule to join the prepared material group (named NIP)

[0082] 1) Experimental steps, respectively weigh 5 mg of nano-artificial antibody material (named MIP) and the prepared material (named NIP) without template molecules, and disperse the material into mannose, fucose, glucose, galactose and In the toluene solution, in order to facilitate UV detection, mannose, fucose, glucose, and galactose were modified with benzyl groups with UV absorption, and the concentration of the solution was 0.1 mg / mL. After shaking at room temperature for 2 hours, centrifuge separation. The material was resuspended and washed 3 times with PBS solution and then dried. The m...

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Abstract

The invention provides a broad-spectrum preparation for inhibiting virus infection and a preparation method thereof, the broad-spectrum preparation prepared based on the method has n mannose binding sites (n is greater than or equal to 60), the preparation can be bound with high mannan multivalence on virus envelope protein, the size of the preparation is nanoscale (30-100nm), and the preparation is of a rigid structure, after being combined with the high mannan on the virus envelope protein, the high mannan antibody blocks the combination of the virus and a host cell receptor through a steric effect, induces the virus aggregation and promotes the removal of the virus by immune cells, so that the broad-spectrum infection inhibition effect on the high mannan-containing virus is realized, and the high mannan antibody has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of pharmacy, in particular to an artificially synthesized nanometer artificial antibody with broad-spectrum antiviral effect. Background technique [0002] Infectious diseases caused by viral infections pose a major threat to human life and health. Many enveloped viruses, including HIV, Influenza, Lassa, Severe Acute Respiratory Syndrome, Zika, Dengue, and Ebola, utilize host cell glycan fragments during host cell replication to Modification of their proteins results in extensive glycosylation of proteins on the viral envelope. Host cell-derived glycans play a functional role in the viral life cycle. In particular, extensively glycosylated viral proteins aid in escaping the immune surveillance of infected hosts by coating the surface of immunogenic proteins with a dense layer of host-derived glycans. Since many viral glycoproteins do not follow the canonical secretion pathway, some proteins are directly transported...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P31/14B82Y5/00C08L5/00
CPCA61K31/715A61P31/14B82Y5/00C08L5/00Y02A50/30
Inventor 刘震李迎许舒欣郭展辰陈静然
Owner NANJING UNIV
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