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Method for in-situ remediation of soil polluted by arsenic and tetracycline hydrochloride through bacteria and application

A technology of tetracycline hydrochloride and in-situ repair, which is applied in the field of soil treatment, can solve the problems of difficult to achieve removal effect, difficult to remove high-concentration ozone resistance gene, complex and other problems, and achieve loose structure, high specific surface area, and surface charge high effect

Pending Publication Date: 2022-06-21
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ozone not only has the effect of oxidizing and degrading antibiotics, but also has the effect of removing resistance genes. However, under normal circumstances, water supply plants mainly add ozone in the pre-oxidation stage and before the activated carbon pool, and it is difficult to add high-concentration ozone to achieve resistance genes. purpose of removal
For the treatment of antibiotics in soil, it is often more complicated, and it is difficult to achieve a better removal effect.

Method used

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  • Method for in-situ remediation of soil polluted by arsenic and tetracycline hydrochloride through bacteria and application
  • Method for in-situ remediation of soil polluted by arsenic and tetracycline hydrochloride through bacteria and application
  • Method for in-situ remediation of soil polluted by arsenic and tetracycline hydrochloride through bacteria and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for bacteria in situ repairing arsenic and tetracycline hydrochloride polluted soil, comprising the following steps: adding bacterial liquid to arsenic and antibiotic composite polluted soil to induce bacteria to produce biological manganese oxide, which is combined with trivalent arsenic, pentavalent arsenic in the soil Valence arsenic and tetracycline hydrochloride undergo a series of physical-chemical-biochemical reactions to complete the simultaneous in-situ restoration of soil arsenic and tetracycline hydrochloride.

[0027] Further, the strains are inoculated into the composite polluted soil of arsenic and antibiotics, the strains can induce the production of biological manganese oxide materials in situ, and the strains adopt the strains capable of converting divalent manganese into manganese oxides.

[0028] Specifically, the biological manganese oxide induced by bacteria has strong oxidative properties, which can oxidize and degrade trivalent arsenic and...

Embodiment 2

[0045] 1) Bacterial culture: The disperse Pantoea WHW-3 was connected to the nutrient broth medium at a concentration of 19 g / L. Place it in a shaking incubator at 180 r min -1 (30°C) for 5 days with shaking.

[0046] 2) Restoration process: prepare the bacterial solution according to the liquid-solid ratio of 1.2 (mL / g soil), centrifuge at 6000 rpm for 10 min, discard the medium components, add water to make a bacterial suspension, and add it to 50 g of soil that is compounded with tetracycline hydrochloride. Then add peptone 16mg, yeast extract 4mg, potassium dihydrogen phosphate 2mg, magnesium sulfate 4mg, sodium nitrate 4mg, calcium chloride 2mg, ammonium chloride 2mg, ferric ammonium citrate 20mg, manganese sulfate 4mg, ammonium carbonate 2mg , the water content was adjusted to 35%, and after stirring with a stirrer for 20 minutes, the culture was stabilized in a constant temperature incubator at 25°C.

[0047] In-situ repair efficiency of arsenic in Table 1 Example 2 ...

Embodiment 3

[0051] On the basis of Example 2, the composite contaminated soil of arsenic and antibiotics was continuously adjusted to a moisture content of 35%, placed at room temperature (25° C.), and after culturing for 2 weeks, continued leaching with water.

[0052] In-situ repair efficiency of arsenic in Table 2 Example 3

[0053]

[0054] From the data in Table 2, it can be seen that the content of arsenic in this example is reduced from 0.14 mg / L to 0.05 mg / L after water leaching, and the restoration efficiency is 64.29%.

[0055] The in situ repair efficiency of tetracycline hydrochloride in table 3 embodiment 3

[0056]

[0057] As can be seen from the data in the above table, in this example, the antibiotic content decreased significantly, from 2.54 mg / L in the unrepaired state to 0.66 mg / L, and the repair efficiency was 65.95%.

[0058] As can be seen from the above 3 embodiments: the present invention adds the biological manganese oxide material to the arsenic and antib...

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Abstract

The invention discloses a method for in-situ remediation of soil polluted by arsenic and tetracycline hydrochloride by bacteria, which comprises the following steps: adding a bacterial liquid, a carbon source, divalent manganese and the like into the soil polluted by the arsenic and tetracycline hydrochloride in a combined manner, inducing the bacteria to generate a biological manganese oxide material, and carrying out a series of physical-chemical-biochemical reactions with the arsenic and tetracycline hydrochloride to realize in-situ remediation of the soil polluted by the arsenic and the tetracycline hydrochloride. Adsorption and immobilization of arsenic and degradation of tetracycline hydrochloride are synchronously achieved, and in-situ remediation of arsenic and tetracycline hydrochloride is achieved. The method has the advantages of simple process, low treatment cost, wide application range, no secondary pollution and the like.

Description

technical field [0001] The invention relates to the technical field of soil treatment, in particular to a method and application of bacteria in situ repairing soil contaminated with arsenic and tetracycline hydrochloride. Background technique [0002] The existing technologies that arsenic-contaminated soil remediation relies on mainly include soil leaching, solidification / stabilization, heat treatment, phytoremediation and microbial remediation. Soil leaching is usually the addition of various leaching agents (such as organic or inorganic acids, alkalis, salts and chelating agents) into the soil. Leaching and precipitation can easily cause groundwater pollution, and it is only suitable for soil treatment with small area and heavy pollution. Thermal treatment of arsenic-contaminated soil also destroys soil structure, and also faces high treatment and operating costs. Solidification / stabilization technology has many applications in arsenic-contaminated soil engineering trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/105B09C1/08
Inventor 王亚楠刘粮丰王华伟孙英杰刘梦凡王祁赵辛龙段钰而莫泽旭刘裔凡
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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