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Lactobacillus johnsonii SXDT-23 and application thereof

A technology of SXDT-23 and Lactobacillus johnsonii, which is applied in the field of microorganisms, can solve the problems of piglet diarrhea incidence and mortality increase, and achieve the effect of improving intestinal immunity, reducing diarrhea rate, and good inhibition

Pending Publication Date: 2022-07-29
INST OF ANIMAL SCI CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ban on antibiotics led to a sharp increase in the incidence of diarrhea and mortality in piglets

Method used

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  • Lactobacillus johnsonii SXDT-23 and application thereof
  • Lactobacillus johnsonii SXDT-23 and application thereof
  • Lactobacillus johnsonii SXDT-23 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Lactobacillus johnsonii ( Lactobacillus johnsonii ) isolation and 16S rRNA identification

[0038] Isolation and identification of Lactobacillus johnsonii:

[0039] Lactobacillus johnsonii (Lactobacillus johnsonii ) was isolated from horse body pig manure in the northern four seasons pasture in the ancient town of Yanggao County, Datong City, Shanxi Province.

[0040] 1. Feces collection: Collect 1 g of feces from 20 fattening horse body pigs, add 20 ml of sterile saline containing 20% ​​glycerol, vortex and mix, and distribute them into sterile cryopreservation tubes, each tube of 1 ml. Store in dry ice after processing, bring back to the laboratory and store at -80°C until use.

[0041] 2. Bacterial isolation: take 1 ml of horse body pig feces, carry out a 10-fold gradient dilution with sterile saline, draw 0.1 mL of a suitable dilution and spread it evenly on MRS agar, after culturing at 37 ° C for 48 hours, the growth The obtained colonies were inocul...

Embodiment 2

[0044] Example 2 Lactobacillus johnsonii ( Lactobacillus johnsonii ) feature detection

[0045] 1. Antibacterial ability test

[0046] The pathogenic indicator bacteria were selected from two common pathogenic bacteria: Escherichia coli ( Escherichia coli ) BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) ATCC14028.

[0047] The antibacterial activity of the strains was determined by the Oxford cup method. Escherichia coli BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) ATCC14028 were respectively inoculated in LB broth medium, placed in a 37°C incubator for 12-18h. Escherichia coli ( Escherichia coli ) BW25113 and Salmonella typhimurium ( Salmonella typhimurium ) The concentration of ATCC14028 strain was adjusted to 1×10 with sterile saline 8 Take 200 μl of CFU / mL each, spread them evenly on LB agar medium, put the sterilized Oxford cup evenly on the LB medium, and add 200 μL of activated bacteria to the Oxford cup and the concentration...

Embodiment 3

[0074] Example 3 Effects of Lactobacillus johnsonii SXDT-23 on intestinal health of DSS model mice

[0075] Through the above experiments, it was determined that Lactobacillus Roche ( Lactobacillus johnsonii ) SXDT-23 strain has the best anti-stress and probiotic properties. Therefore, Lactobacillus Roche ( Lactobacillus johnsonii ) SXDT-23 strain was used for subsequent experiments.

[0076] 1. Experimental method

[0077] 1. 36 20-day-old ICR male mice were selected and raised in the Animal Center of the Institute of Animal Science, Chinese Academy of Agricultural Sciences. During the experiment, the temperature of the mouse room was controlled at 21±2°C, the light / dark time was 12 hours, and food and water were freely taken.

[0078] 2. The mouse colon inflammation injury model was established with dextran sodium sulfate (DSS). All mice were randomly divided into three groups: Con group, DSS group, L.j+DSS group. Con group: During the experiment, 0.1ml of normal saline...

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Abstract

The invention relates to the field of microorganisms, in particular to lactobacillus johnsonii SXDT-23 and application thereof. According to the invention, a strain of Lactobacillus johnsonii SXDT-23 is obtained by separating from pig manure of a horse body, and the strain has a very good inhibition effect on escherichia coli and salmonella; the strain can be colonized in an animal body, and has strong tolerance to artificial cholate, artificial gastric acid and artificial intestinal juice; the feed can promote animal growth, improve colitis, reduce diarrhea rate, improve intestinal immunity and improve intestinal health.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Lactobacillus johnsonii SXDT-23 and applications thereof. Background technique [0002] The stress caused by early weaning is mainly manifested as piglet diarrhea, resulting in reduced piglet growth performance and increased mortality. Although antibiotics have significantly improved the efficacy of diarrheal treatment over the past few decades, the development and spread of drug-resistant strains, often resulting in antibiotic residues in food, has become a serious problem. As a result, many countries are phasing out the use of antibiotics in animal production. However, the prohibition of antibiotics led to a sharp increase in the incidence of diarrhea and mortality in piglets. Therefore, there is an urgent need to develop green, safe, efficient and non-toxic treatment methods for diarrhea. [0003] At present, the search for alternatives to antibiotics has become a research hot...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P1/00A61P1/12A61P37/04A23K10/18A23K50/30C12R1/225
CPCC12N1/20A61K35/747A61P31/04A61P1/00A61P1/12A61P37/04A23K10/18A23K50/30A23V2002/00A23V2400/151A23V2200/32A23V2200/324A23V2200/3204Y02A50/30
Inventor 陈亮骆城增张宏福
Owner INST OF ANIMAL SCI CAAS
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