Application of antraquinone derivative as pesticide for controlling plant diseases
An anthraquinone derivative and plant disease technology, used in the field of pesticides for preventing and controlling plant fungal diseases
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Embodiment 1
[0020] Example 1: Research on the effect of derivative compound 7 (Emodin-1,6-dimethylether) of the present invention on the development of wheat powdery mildew at seedling stage.
[0021] 1. Bacterial species: wheat powdery mildew (Blumeria graminis f.sp.tritici) strain No14, which was determined to be highly pathogenic by testing 10 main wheat varieties in Hubei.
[0022] 2. Wheat variety: Zheng 98 (shows affinity with No14 strain).
[0023] 3. Cultivation of wheat seedlings: Cultivate wheat seedlings with consistent growth in a 18±1°C light incubator with a flower pot with a diameter of 30 cm. There are 15 plants in each pot. When the seedlings grow to one leaf and one heart, the experiment is carried out, and there are two kinds of potted plants and isolated leaves. Methods Sampling microscopic examination after inoculation. The isolated leaf segment inoculation method is as follows: the first leaf is cut into 3 cm leaf segments respectively, and placed on 0.5% water agar...
Embodiment 2
[0028] Embodiment 2, research on the inhibitory effect of derivative compounds of the present invention on wheat powdery mildew
[0029] The same method as in Example 1 is used to cultivate powdery mildew strains and wheat seedlings, and the derivative compounds 1-10 are dissolved in ethanol and benzene respectively and then diluted to different concentration gradients (100ug / g~1.25ug / g) to measure their EC 50 value, and use 6.25ug / g concentration to measure its potted plant seedling activity. Put the detached leaves and plant seedlings in a micro-spray tower, spray 3ml per concentration, repeat 3 times, inoculate powdery mildew after 6 hours of spraying, the inoculation amount is the same as that in Example 1, and then place it under light at 18±1°C Cultivate in the cultivation room, record the number of powdery mildew infection points on each leaf segment and leaf after 6 days, and calculate the antibacterial effect (%) of each treatment. The result proves that compound 1-1...
Embodiment 3
[0031] Embodiment 3: Research on the inhibitory effect of derivative compounds of the present invention on rice blast fungus
[0032] 1. Strain: Pyricularia grisea w02-1 (compatible with Lijiang Xintuan Heigu)
[0033] 2. Rice variety: Lijiang Xintuan Heigu (does not contain any blast resistance gene)
[0034] The inhibitory activity of 1-10 derivative compounds against Magnaporthe oryzae was determined by spore germination method. Tomato-oat juice medium was used to cultivate w02-1, each liter of medium contained 150ml tomato juice, 750ml oat juice (30g oatmeal, 1000ml water, boiled for 20min, filtered with double gauze), and 18g agar. Insert the w02-1 mycelia after sterilization, cultivate for 2 days under light at 25°C, interrupt the aerial mycelium with a sterilized cotton swab, wash the spores with sterile water after continuing to cultivate for 1-2 days, filter them with gauze, Dilute the spore suspension to 1 x 10 6pcs / ml for use. After dissolving the compounds 1-10...
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