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Method of preparing anticoagulant biological material using electrostatic self-assembling

A technology of electrostatic self-assembly and biomaterials, which is applied in anticoagulation treatment, packaging item types, special packaging items, etc., can solve the problems of complex process, solvent toxicity, poor controllability, etc., and achieve simple process, high resistance Coagulation biological activity, mild effect of preparation method

Inactive Publication Date: 2003-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common weaknesses of these surface modification methods, such as solvent toxicity, complicated process, and poor controllability, not only the designability of the material surface is greatly limited, but also the modification of medical devices with complex geometric shapes cannot be realized. Meet the needs of the rapid development of medical devices

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] First immerse the medical 316L stainless steel in 0.01-2mg / ml polyethyleneimine aqueous solution for 5-10 minutes, wash it with deionized water, and dry it with nitrogen to obtain the surface of polyethyleneimine physical adsorption, which is positively charged under the condition of aqueous solution . Immerse the positively charged material above in 0.01-10 mg / ml heparin sodium aqueous solution for 5-10 minutes, wash it with deionized water, and dry it with nitrogen gas to obtain the surface of the charged polyanion anticoagulant heparin; the negatively charged polyanion anticoagulant Immerse the surface of coagulation heparin in 0.01-20 mg / ml polyethyleneimine aqueous solution for 10-20 minutes, wash it with deionized water, and blow it dry with nitrogen gas to obtain a surface charged with polycations. Repeat the above process to obtain polyanion anticoagulation heparin and 2-10 layers of polycationic polyethyleneimine alternately coat the surface. It was found by c...

Embodiment 2

[0023] First immerse medical 316L stainless steel in 10-50mM 3-aminopropyltriethoxysilane ethanol solution, after soaking for 3 hours, transfer the stainless steel to 0.1M hydrochloric acid solution, and hydrolyze the 3-ammonia adsorbed on the stainless steel Propyltriethoxysilane for 20 hours, wash off the hydrochloric acid on the surface with ethanol and deionized water, then blow dry with nitrogen, and cure for 1 hour under nitrogen protection at 110 degrees. The surface was immersed in an aqueous hydrochloric acid solution with a pH of 2.0, then deionized and cleaned, and dried with nitrogen to obtain a stable positively charged surface. Immerse the positively charged material above in 20-40 mg / ml sodium heparin aqueous solution for 20-30 minutes, wash it with deionized water, and dry it with nitrogen gas to obtain the surface of the charged polyanion anticoagulant heparin; the negatively charged polyanion anticoagulant Immerse the surface of coagulation heparin into 1-5 m...

Embodiment 3

[0025] Wash the medical PVC film with absolute ethanol and deionized water respectively, dry it with nitrogen, and then dry it in vacuum. Treat PVC film with ammonia gas plasma: put the dried PVC film in the plasma reactor, evacuate the plasma reactor, and maintain the pressure of the plasma treated substrate at 40-50Pa by passing ammonia gas, discharge current 25 ~ 100mA, processing time 5 ~ 15min. Then the reactor was returned to normal pressure, and the treated PVC film was taken out. Its surface contains amino functional groups. Immerse the PVC film treated with ammonia gas plasma in a hydrochloric acid aqueous solution with a pH of 0.5-2 for 5-15 minutes to convert the amino hydrochloride into ammonia ions, take out deionized water for cleaning, and blow dry with nitrogen to obtain a positively charged surface. Immerse the positively charged above-mentioned material in 50-70 mg / ml albumin PBS buffer solution (pH7.4) for 40-50 minutes, wash with deionized water, and blow...

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PUM

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Abstract

The electrostatic self-assembling process of preparing anticoagulant biological material includes the following steps: obtaining positive or negative charges on the surface of biological material viasurface modifying course; soaking the charged material into water solution of polyelectrolyte of concentration 0.01-10 mg / ml and with opposite charges for adsorbing for 5-60 min, washing with distilled water and air drying; soaking the dried material into water solution of polyelectrolyte of concentration 0.01-10 mg / ml and with opposite charges for adsorbing for 5-60 min, washing with distilled water and air drying; repeating the steps alternately to obtain several layers of alternate positive and negative charge polyelectrolytes on the surface including one or several kinds of charged anticoagulant medicine. The present invention can realize the coating modification of biomedical material with complex structure to improve the anticoagulant performance of the material.

Description

technical field [0001] The invention relates to a method for preparing anticoagulant biological material by electrostatic self-assembly. Background technique [0002] The blood environment is one of the most important environments for the human body. Good blood compatibility materials can be widely used in various medical interventional devices in contact with blood, blood purification and dialysis devices, extracorporeal circulation devices and artificial lungs, artificial livers, artificial Various artificial organs such as the heart. has great clinical significance. However, most synthetic materials will non-specifically induce the adsorption of various proteins (including coagulation factors) when in contact with blood, induce the generation of thromboplastin through the intrinsic and extrinsic coagulation pathways, activate platelets, and finally lead to coagulation. [0003] Biomaterials with good anticoagulant properties have been prepare...

Claims

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Application Information

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IPC IPC(8): A61L33/08A61L33/12A61L33/16
Inventor 计剑谭庆刚沈家骢
Owner ZHEJIANG UNIV
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