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Preparation method of rape myricyl powder effective portion group

A technology of rapeseed bee pollen and effective parts, which is applied in the field of preparation of effective parts of rapeseed bee pollen, can solve the problems of low content, increased dosage, and difficulty in establishing compliance, etc., and achieves abundant and easy access to resources, improved safety, and enhanced The effect of competitiveness

Inactive Publication Date: 2005-08-10
ZHEJIANG CONBA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing anti-prostatic hyperplasia pollen preparation produced in Sweden is composed of 26% Timothy grass, 26% corn, 19% rye, 26% hazel, 6% salicylic, 6% poplar, 6% French chrysanthemum and 5% pine. It is made of mixed extraction and preparation of plant pollen, which is expensive
[0006] Since the current domestic pollen preparations are directly produced from rapeseed pollen, the dosage is large and it is not easy to store
[0007] Some people in China have proposed to use pollen (the source plant of the pollen) extract as the raw material of pharmaceutical preparations to prepare anti-prostatic hyperplasia drugs, but if the source plant of the pollen is not sure, the three basic requirements of drug effectiveness, safety and stability cannot be guaranteed
For example, Chinese patent CN 1391911A describes a method in which high-efficiency pollen is used as a raw material to obtain anti-prostatic hyperplasia pollen extract by cold soaking in acetone. This method has the following disadvantages: (1) the pollen used for the raw material preparation is not clear about the plant source, Difficult to establish compliance with the basic requirements as a drug
(2) Because acetone is volatile and the recovery rate is extremely low, large-scale production such as using acetone as the extraction solvent has high production costs
(3) Due to the extraction with acetone, the extract will be used as a source of pharmaceutical preparations without separation and purification, so that the non-effective ingredients in the extract cannot be removed, and the content of active ingredients in the extract is low, which will increase the dosage and bring harm to patients. Inconvenience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Through characteristic color reaction and thin-layer chromatography inspection, the fat-soluble active part of rape bee pollen of the present invention contains a large amount of sterols, plant fatty acids, a small amount of sugar and amino acid, and the water-soluble active part contains sugar, flavonoids and amino acid substances. Liquid chromatography measures the content of fat-soluble active ingredient sterols in the antiprostatic hyperplasia drug of the present invention:

[0013] Instrument: Waters High Performance Liquid Chromatograph

[0014] Column: C 18 Column 4.6mm×250mm

[0015] Mobile phase: acetonitrile-water (97:3)

[0016] Detection wavelength: 210nm

[0017] Flow rate: 1ml / min

[0018] Results: The content of sterols was found to be 15%-35%.

[0019] Amino acid content in the rape bee pollen effective part of the present invention is measured with an amino acid analyzer: detection wavelength: 540nm, 470nm, condition: exchange resin, Japanese-made ...

Embodiment 2

[0022] Example 2 Pharmacological test of effective parts of rapeseed pollen of the present invention

[0023] 1. 500 grams of fat-soluble effective parts and 510 grams of water-soluble effective parts (by crude drug extraction amount) are mixed, and after SD rats are adaptively raised for one week, intraperitoneal injection of 0.3% pentobarbital sodium 0.1ml / kg is anesthetized, Laparotomy was performed, bilateral testes were removed, and 10 rats were randomly selected as the sham operation group. One week after the operation, the castrated animals were randomly divided into model control group, positive drug control group, and experimental drug, with 10 animals in each group. Except for the sham operation group, the rats in the other three groups were subcutaneously injected with testosterone propionate injection, 2.0 mg / kg, once a day for 30 consecutive days. At the same time as the injection of modeling drugs, the doses in the table below were given by intragastric administ...

Embodiment 3

[0039] Take 1000 g of rapeseed pollen, dry it, grind it mechanically, soak and extract it with 95% ethanol five times. Each time for 24 hours, the amount of ethanol added is 3000ml each time, the extract is combined, and the extract is concentrated to a thickness of about 300ml to obtain a fat-soluble extract. Add 600ml of 50-60% ethanol to the fat-soluble extract, mix well and add petroleum Ether extracted 2-4 times, 1500ml each time, combined, the extract was recovered to dryness, and 114g of fat-soluble active fraction was obtained.

[0040]After ethanol leaching, the pollen was evaporated to remove ethanol, soaked in purified water and extracted four times, each time for 8 hours, and the amount of purified water added was 3000ml each time, and the extract was filtered and concentrated to 900ml to obtain a water-soluble extract. Add 450ml of 95% ethanol to the extract for alcohol precipitation overnight, filter, recover the filtrate to 1000ml, add 2000ml of 95% ethanol for ...

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PUM

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Abstract

A process for preparing the active components of rape melissa powder includes pulverizing or wall-breaking, extracting in alcohol or propanol, concentrating the liquid extract, immersing the dregs in solvent, evaporating solvent, extracting in water, concentrating the liquid extract, dissolving liposoluble extract in alcohol, concentrating, drying to obtain water-soluble active components, mixing them together, and shaping.

Description

technical field [0001] The invention relates to a method for preparing rape pollen effective fractions from rape (Brassica campestris L) bee pollen (Pollen of Brassin Campestris L). The main raw material of drugs related to symptoms such as frequent urination, urgency, dysuria, dribbling after urination, urinary retention, white urethra, and sexual dysfunction. Background technique [0002] The prostate is the accessory gonad of men, and the prostatic fluid secreted by it is an important part of semen. It is shaped like a chestnut and sits just below the bladder, with the urethra passing right through it. Prostatic hyperplasia is the most common disease unique to chronic male elderly. After a person reaches middle age, the connective tissue in the prostate begins to proliferate. In severe cases, the volume of the gland can be 10-15 times larger than the normal gland. The hypertrophy of the prostate stimulates and compresses, causing frequent urination, dribbling, difficult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K9/10A61K9/48A61P13/08
Inventor 张冯敏阮碧芳吴健邱红英姚建标
Owner ZHEJIANG CONBA PHARMA
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