Preparation method of rape myricyl powder effective portion group
A technology of rapeseed bee pollen and effective parts, which is applied in the field of preparation of effective parts of rapeseed bee pollen, can solve the problems of low content, increased dosage, and difficulty in establishing compliance, etc., and achieves abundant and easy access to resources, improved safety, and enhanced The effect of competitiveness
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Embodiment 1
[0012] Through characteristic color reaction and thin-layer chromatography inspection, the fat-soluble active part of rape bee pollen of the present invention contains a large amount of sterols, plant fatty acids, a small amount of sugar and amino acid, and the water-soluble active part contains sugar, flavonoids and amino acid substances. Liquid chromatography measures the content of fat-soluble active ingredient sterols in the antiprostatic hyperplasia drug of the present invention:
[0013] Instrument: Waters High Performance Liquid Chromatograph
[0014] Column: C 18 Column 4.6mm×250mm
[0015] Mobile phase: acetonitrile-water (97:3)
[0016] Detection wavelength: 210nm
[0017] Flow rate: 1ml / min
[0018] Results: The content of sterols was found to be 15%-35%.
[0019] Amino acid content in the rape bee pollen effective part of the present invention is measured with an amino acid analyzer: detection wavelength: 540nm, 470nm, condition: exchange resin, Japanese-made ...
Embodiment 2
[0022] Example 2 Pharmacological test of effective parts of rapeseed pollen of the present invention
[0023] 1. 500 grams of fat-soluble effective parts and 510 grams of water-soluble effective parts (by crude drug extraction amount) are mixed, and after SD rats are adaptively raised for one week, intraperitoneal injection of 0.3% pentobarbital sodium 0.1ml / kg is anesthetized, Laparotomy was performed, bilateral testes were removed, and 10 rats were randomly selected as the sham operation group. One week after the operation, the castrated animals were randomly divided into model control group, positive drug control group, and experimental drug, with 10 animals in each group. Except for the sham operation group, the rats in the other three groups were subcutaneously injected with testosterone propionate injection, 2.0 mg / kg, once a day for 30 consecutive days. At the same time as the injection of modeling drugs, the doses in the table below were given by intragastric administ...
Embodiment 3
[0039] Take 1000 g of rapeseed pollen, dry it, grind it mechanically, soak and extract it with 95% ethanol five times. Each time for 24 hours, the amount of ethanol added is 3000ml each time, the extract is combined, and the extract is concentrated to a thickness of about 300ml to obtain a fat-soluble extract. Add 600ml of 50-60% ethanol to the fat-soluble extract, mix well and add petroleum Ether extracted 2-4 times, 1500ml each time, combined, the extract was recovered to dryness, and 114g of fat-soluble active fraction was obtained.
[0040]After ethanol leaching, the pollen was evaporated to remove ethanol, soaked in purified water and extracted four times, each time for 8 hours, and the amount of purified water added was 3000ml each time, and the extract was filtered and concentrated to 900ml to obtain a water-soluble extract. Add 450ml of 95% ethanol to the extract for alcohol precipitation overnight, filter, recover the filtrate to 1000ml, add 2000ml of 95% ethanol for ...
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