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Tissur engineering blood vessel and method of construction in vitro

A technology of tissue engineering and blood vessels, applied in blood vessels, devices of human tubular structures, prostheses, etc., can solve problems such as difficulty in ensuring the structural integrity of scaffolds, and achieve the effect of enhancing anticoagulant ability and promoting cell adhesion

Inactive Publication Date: 2006-01-11
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method of making tubes separately, culturing cells separately and then nesting them together will hardly ensure the structural integrity of the scaffold: there will be obvious gaps between the inner tube and the outer tube

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Biomimetic tissue engineered blood vessels that can release growth factors

[0023] (1) Cultivation, passage and identification of seed cells:

[0024] Endothelial cells: cultured by Jeff enzyme digestion method. The umbilical cord of a healthy puerpera within 6 hours of delivery was taken, soaked in normal saline, a silicone tube was inserted at the entrance of the umbilical vein at one end, and the suture was tied tightly. The other end of the silicone tube was ligated in the same way, injected with PBS buffer, and washed repeatedly until the buffer was free of blood. Perfuse 0.1-0.15wt% type I collagenase into the vein cavity, incubate at 37°C for 15-20min, collect the precipitated cells after centrifugation, add M199 culture medium (containing 20% ​​FBS, 2×10 5 U / L penicillin, 0.12mg / L streptomycin, 20-25mg / L VEGF), blow off the cells, inoculate into gelatin pretreated culture flasks, place at 37°C, 5% CO 2 Static culture in the cell culture incubator. After the...

Embodiment 2

[0035] Tissue engineered blood vessels with anticoagulant function

[0036] Tissue engineered blood vessels were constructed in the same steps as in Example 1, and the cultivation and passage of seed cells were the same. The difference is that when the vascular media layer is constructed, the shell layer is made of heparinized type I collagen, and the core is made of PGA or PLGA, which are prepared with hexafluoroisopropanol (HFP) and a 1:1 THF / DMF mixed solvent. Shell-core electrospinning solution; when constructing the vascular adventitia, the shell is made of silkworm chrysalis protein and the core is PLLA, and the shell-core electrospun is prepared with formic acid solution and 1:1-1:3 acetone / chloroform mixed solvent respectively. solution.

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Abstract

A histoengineered blood vessel features that its internal and external layers are made of the composite superfine fibre membrane and different cells are transplanted in different layers. Said composite superfine fibre membrane is composed of a core made of natural or artificial biodegradable material and the shell layer made of natural biodegradable material. Its external construction method includes such steps as preparing scaffold step by step, transplanting cells layer by layer, and integrally shaping.

Description

technical field [0001] The invention relates to a tissue engineering blood vessel for medical use and an in vitro construction method thereof. More specifically, it relates to a tissue engineered blood vessel with a multi-layer composite ultrafine fiber structure and a bioactive component that can be released by using coaxial co-spinning technology. Background technique [0002] Cardiovascular disease is one of the most serious diseases threatening human health. Defective and severely diseased blood vessels are generally transplanted and reconstructed. At present, nearly one million vascular reconstruction operations are performed every year in the world, and the clinically used vascular grafts include autologous, allogeneic, xenograft and artificial polymer blood vessels. Although these grafts have their own advantages, they all have defects such as insufficient donors, immune rejection, and thrombus formation. The development of tissue engineering has provided a new way ...

Claims

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Application Information

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IPC IPC(8): A61F2/06A61L27/38A61L27/50
Inventor 黄争鸣何创龙张彦中
Owner TONGJI UNIV
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