Use of molo glycoside in alpha-glucosaccharase inhibitor
A glucosidase and moroside technology, which is applied in the field of α-glucosidase inhibitor and moroside preparation, can solve the problem of not finding α-glucosidase inhibitory effect, not suitable for mass preparation and industrial production, and moroside. The unstable nature of glycosides and other problems can achieve the effect of controlling postprandial blood sugar, low cost and good glucose tolerance.
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Examples
experiment example 1
[0026] Experimental Example 1 Inhibition of morroside on α-glucosidase
[0027] Purpose of the experiment: To observe the inhibitory effect of morroside on α-glucosidase in vitro by taking alglucoside as a positive control, and to provide in vitro test basis for the study of its mechanism of reducing postprandial blood sugar and improving glucose tolerance.
[0028] Experimental method: Morroside (self-made, content 97.6%) was prepared into 20, 8, 3.2, 1.28, 0.512, 0.2048, 0.08192, 0.032768, 0.013107, 0.005243 μg / μl test solutions with 0.1M phosphate buffer. Mix 40 μl of the test solution with 10 μl α-glucosidase (sigma, batch number WA15194), incubate at 37°C for 10 minutes, add 50 μl of 2.0 mmol / L maltose, continue to incubate at 37°C for 10 minutes, and terminate the reaction in a boiling water bath. The OD value was measured at 490nm by an oxidase microplate reader (BIO-RAD).
[0029] Positive control: Baitangping (Beijing Bayer Medicine and Health Care Co., Ltd., batch n...
experiment example 2
[0033] Experimental example 2 Research on the inhibition type of α-glucosidase by moroside
[0034] Purpose of the experiment: To study the type of inhibition of morroside on α-glucosidase in vitro, and to provide in vitro test basis for the study of its mechanism of lowering postprandial blood sugar and improving glucose tolerance.
[0035] Experimental method: Morroside (self-made, content 97.6%) was prepared into 20, 8 μg / μl test solution with 0.1M phosphate buffer. Under the condition of certain inhibitor concentration, the substrates were added respectively (the concentration is 0.25, 0.5, 1, 2, 3, 4, 5 mmol / l), and the enzyme activity was measured. By changing the inhibitor concentration, the enzyme activity under a series of different substrate concentrations can be obtained. The type of inhibition was determined by Lineweaver-Burk plot.
[0036] Experimental results: Lineweaver-Burk mapping ( figure 1 ) results showed that the inhibition type of α-glucosidase by mor...
experiment example 3
[0037] Experimental Example 3 Effect of Morroside on Blood Glucose, Glycated Serum Protein and Hepatic Glycogen in Streptozotocin Model Rats.
[0038]The purpose of the experiment: to observe the effect of oral administration of Cornus officinalis extract containing 30% morroside on the blood sugar, glycosylated serum protein and liver glycogen levels of streptozotocin model rats, using glucocorticoid as a positive control, for Clinical medication provides the basis for animal experiments.
[0039] Experimental method: Rats were fasted for 24 hours, each ip streptozotocin 70mg / kg, 0.5ml / 100g body weight. After 3 days of normal feeding, fasting for 12 hours, measure fasting serum glucose (FSG), the method is the same as before, FSG greater than 16.7mmol / L is a hyperglycemic rat. According to the blood sugar level, the hyperglycemic rats were divided into 5 groups, 10 in each group, respectively: model control group, positive drug group, test drug high, middle and low dose grou...
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Application Information
- IPC
- A61K31/7048; A61P3/04; A61P3/10; C07H1/08; C07H17/04
- Inventors
- 印敏; 楼凤昌